Molecular characteristics and epidemiology of coxsackievirus A06-associated Hand Foot and Mouth Disease: Data from 10 years of enterovirus surveillance in Denmark, 2005–2014

2015 ◽  
Vol 70 ◽  
pp. S4-S5
Author(s):  
S. Midgley ◽  
P.H. Andersen ◽  
B. Bottiger ◽  
M.W. Poulsen ◽  
T.K. Fischer
Keyword(s):  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Molecular Characteristics ◽  
Foot And Mouth

scholarly journals A five-year retrospective study on the epidemiology of hand, foot and mouth disease in Sabah, Malaysia

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Siat Yee Fong ◽  
Daisuke Mori ◽  
Christina Rundi ◽  
Jun Fai Yap ◽  
Muhammad Jikal ◽  
...  
Keyword(s):  
Disease Surveillance ◽  
Phylogenetic Analyses ◽  
Foot And Mouth Disease ◽  
Study Data ◽  
Mouth Disease ◽  
Oligonucleotide Primer ◽  
Nucleotide Sequencing ◽  
Molecular Characteristics ◽  
Disease Surveillance System ◽  
Foot And Mouth

AbstractHand, foot, and mouth disease (HFMD) is endemic in Malaysia, with the number of cases increasing. Sabah has experienced several HFMD outbreaks, but information on the epidemiology and molecular characteristics of responsible viruses is scarce. In this study, data of 17,574 reports of HFMD cases in Sabah from 2015 to 2019 were extracted from a public health disease surveillance system and analyzed. Twenty-one swab samples from 13 children were collected from Beaufort, Sabah, during an outbreak in August 2018 for detection and serotyping of causative viruses by semi-nested reverse transcription-polymerase chain reaction (snRT-PCR) of the VP4–VP2 region and consensus degenerate hybrid oligonucleotide primer PCR of the VP1 region, respectively. Nucleotide sequencing and phylogenetic analysis were conducted by the neighbor-joining method. The average annual incidence of HFMD was 94.3 per 100,000 people, with the greatest yearly increase between 2017 and 2018. Swabs from six children were tested positive for enterovirus, of which five were positive for CVA16 and one for EV71. All CVA16 strains belonged to sub-genotype B1a, and the EV71 strain belonged to sub-genotype B5. Phylogenetic analyses indicate that enterovirus genotype shift might be responsible for the increasing trend of HFMD in Sabah, however, further study is needed.

scholarly journals Molecular characteristics of hand, foot, and mouth disease for hospitalized pediatric patients in Yunnan, China

Medicine ◽  
2018 ◽  
Vol 97 (31) ◽  
pp. e11610 ◽  
Author(s):  
Yilin Zhao ◽  
Haihao Zhang ◽  
Hongbo Liu ◽  
Jie Zhang ◽  
Licun He ◽  
...  
Keyword(s):  
Pediatric Patients ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Molecular Characteristics ◽  
Foot And Mouth

Biophysical Measurement of Molecular Weight of Foot-and-Mouth Disease RNA Fragments

1974 ◽  
Vol 32 ◽  
pp. 262-263
Author(s):  
Sydney S. Breese ◽  
Howard L. Bachrach
Keyword(s):  
Chemical Properties ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Distilled Water ◽  
Bovine Plasma ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Carbon Coated ◽  
Rna Fragments ◽  
Physical And Chemical

Continuing studies on the physical and chemical properties of foot-and-mouth disease virus (FMDV) have included electron microscopy of RNA strands released when highly purified virus (1) was dialyzed against demlneralized distilled water. The RNA strands were dried on formvar-carbon coated electron microscope screens pretreated with 0.1% bovine plasma albumin in distilled water. At this low salt concentration the RNA strands were extended and were stained with 1% phosphotungstic acid. Random dispersions of strands were recorded on electron micrographs, enlarged to 30,000 or 40,000 X and the lengths measured with a map-measuring wheel. Figure 1 is a typical micrograph and Fig. 2 shows the distributions of strand lengths for the three major types of FMDV (A119 of 6/9/72; C3-Rezende of 1/5/73; and O1-Brugge of 8/24/73.

High resolution surface structure of foot-and-mouth disease virus

1978 ◽  
Vol 36 (2) ◽  
pp. 376-377
Author(s):  
S. S. Breese ◽  
H. L. Bachrach
Keyword(s):  
Electron Microscopy ◽  
High Resolution ◽  
Surface Structure ◽  
Disease Virus ◽  
Potassium Phosphate ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Physical Measurements ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Models for the structure of foot-and-mouth disease virus (FMDV) have been proposed from chemical and physical measurements (Brown, et al., 1970; Talbot and Brown, 1972; Strohmaier and Adam, 1976) and from rotational image-enhancement electron microscopy (Breese, et al., 1965). In this report we examine the surface structure of FMDV particles by high resolution electron microscopy and compare it with that of particles in which the outermost capsid protein VP3 (ca. 30, 000 daltons) has been split into smaller segments, two of which VP3a and VP3b have molecular weights of about 15, 000 daltons (Bachrach, et al., 1975).Highly purified and concentrated type A12, strain 119 FMDV (5 mg/ml) was prepared as previously described (Bachrach, et al., 1964) and stored at 4°C in 0. 2 M KC1-0. 5 M potassium phosphate buffer at pH 7. 5. For electron microscopy, 1. 0 ml samples of purified virus and trypsin-treated virus were dialyzed at 4°C against 0. 2 M NH4OAC at pH 7. 3, deposited onto carbonized formvar-coated copper screens and stained with phosphotungstic acid, pH 7. 3.

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