An electrochemical amperometric immunobiosensor for label-free detection of α-fetoprotein based on amine-functionalized graphene and gold nanoparticles modified carbon ionic liquid electrode

2011 ◽  
Vol 656 (1-2) ◽  
pp. 72-77 ◽  
Author(s):  
Ke-Jing Huang ◽  
De-Jun Niu ◽  
Jun-Yong Sun ◽  
Jun-Jie Zhu
Micromachines ◽  
2021 ◽  
Vol 12 (10) ◽  
pp. 1256
Author(s):  
Pingping Xu ◽  
Wenpo Feng ◽  
Mei Wang ◽  
Ling Zhang ◽  
Gaofeng Liang ◽  
...  

The early detection of tumor markers has an effective role in the treatment of cancer. Here, a new sandwich-type electrochemical immunosensor for early label-free detection of the cancer biomarker carcinoembryonic antigen (CEA) was developed. Dendritic tri-fan blade-like PdAuCu nanoparticles (PdAuCu NPs)/amine functionalized graphene oxide (NH2-GO) were the label of secondary antibodies (Ab2), and Au nanoparticle-decorated polydopamines (Au/PDA) were immobilized on a screen-printed carbon electrode (SPCE) as the substrate materials. Dendritic tri-fan blade-like PdAuCu NPs/NH2-GO was synthesized according to a simple hydrothermal procedure and used to immobilize antibodies (Ab2) with large surfaces areas, increased catalytic properties and good adsorption to amplify the current signals. Subsequently, Ab2/PdAuCu NPs/NH2-GO catalyzed the reduction of H2O2 in the sandwich-type immunoreactions. Under optimal conditions, the immunosensor exhibited a satisfactory response to CEA with a limit detection of 0.07 pg mL−1 and a linear detection range from 0.1 pg mL−1 to 200 ng mL−1. The proposed immunosensor could be suitable enough for a real sample analysis of CEA, and has clinical value in the early diagnosis of cancer.


2015 ◽  
Vol 7 (5) ◽  
pp. 1878-1883 ◽  
Author(s):  
Wencheng Wang ◽  
Yong Cheng ◽  
Lijun Yan ◽  
Huanhuan Zhu ◽  
Guangjiu Li ◽  
...  

A highly sensitive electrochemical sensor for the detection of dopamine was realized with a double-stranded deoxyribonucleic acid/gold nanoparticles/graphene modified carbon ionic liquid electrode.


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