Internal controls for quantitative RT-PCR analysis of gene expression in response to ocean acidification in edible oysters

2022 ◽  
Vol 548 ◽  
pp. 151683
Author(s):  
Xin Dang ◽  
Zohaib Noor ◽  
Yuan-Qiu He ◽  
Yong-Kian Lim ◽  
Yang Zhang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ocean Acidification ◽  
Internal Controls ◽  
Pcr Analysis ◽  
Rt Pcr

Cloning of rabbit prolactin cDNA and prolactin gene expression in the rabbit mammary gland

1996 ◽  
Vol 16 (1) ◽  
pp. 27-37 ◽  
Author(s):  
L Gabou ◽  
M Boisnard ◽  
I Gourdou ◽  
H Jammes ◽  
J-P Dulor ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mammary Gland ◽  
Amino Acid ◽  
Untranslated Regions ◽  
Pcr Analysis ◽  
Cdna Clones ◽  
Rt Pcr ◽  
Prolactin Gene ◽  
New Zealand White Rabbits ◽  
Rna Probe

ABSTRACT cDNA clones coding for rabbit prolactin were isolated from a pituitary library using a rat prolactin RNA probe. One cDNA contained 873 bases including the entire coding sequence of rabbit prolactin, its signal peptide and the 5′ and 3′ untranslated regions of 44 and 145 nucleotides respectively. The deduced amino acid sequence of the cloned prolactin cDNA presented a 93–78% identity with mink, porcine and human prolactins. The prolactin gene transcription was investigated by RT-PCR analysis in several organs of midlactating New Zealand White rabbits. The ectopic transcription of the prolactin gene was examined in more detail in the mammary gland. A strong PCR signal was detected in the mammary gland of virgin does and was also observed during pregnancy and at the beginning of lactation. This PCR signal was very weak in mid-lactating and absent in post-weaning mammary gland.

scholarly journals Microdissection RT-PCR Analysis of Gene Expression in Pathologically Defined Frozen Tissue Sections

BioTechniques ◽  
1996 ◽  
Vol 21 (1) ◽  
pp. 38-44 ◽  
Author(s):  
Tamara Hiller ◽  
Linda Snell ◽  
Peter H. Watson
Keyword(s):  
Gene Expression ◽  
Pcr Analysis ◽  
Rt Pcr ◽  
Tissue Sections ◽  
Frozen Tissue

Chemokines gene expression of RAW 264.7 cells by Actinobacillus actinomycetemcomitans lipopolysaccharide using microarray and RT-PCR analysis

2009 ◽  
Vol 27 (2) ◽  
pp. 257-261 ◽  
Author(s):  
Jin Chung ◽  
Mun Jeoung Choi ◽  
So Yeon Jeong ◽  
Jong Suk Oh ◽  
Hyung Keun Kim
Keyword(s):  
Gene Expression ◽  
Actinobacillus Actinomycetemcomitans ◽  
Raw 264.7 Cells ◽  
Pcr Analysis ◽  
Raw 264.7 ◽  
Rt Pcr
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