Cattle wastewater as a low-cost supplement augmenting microalgal biomass under batch and fed-batch conditions

2022 ◽  
Vol 304 ◽  
pp. 114213
Author(s):  
Rahul Jain ◽  
Sanjeev Mishra ◽  
Kaustubha Mohanty
Keyword(s):  
Low Cost ◽  
Fed Batch ◽  
Microalgal Biomass

scholarly journals The Dark Side of Microalgae Biotechnology: A Heterotrophic Biorefinery Platform Directed to ω-3 Rich Lipid Production

2019 ◽  
Vol 7 (12) ◽  
pp. 670 ◽  
Author(s):  
Teresa Lopes da Silva ◽  
Patrícia Moniz ◽  
Carla Silva ◽  
Alberto Reis
Keyword(s):  
Low Cost ◽  
Lipid Production ◽  
Arable Land ◽  
Integrated System ◽  
Dark Side ◽  
Omega 3 ◽  
Niche Markets ◽  
Microalgal Biomass ◽  
Oleaginous Microalgae ◽  
Heterotrophic Microalgae

Microbial oils have been considered a renewable feedstock for bioenergy not competing with food crops for arable land, freshwater and biodiverse natural landscapes. Microalgal oils may also have other purposes (niche markets) besides biofuels production such as pharmaceutical, nutraceutical, cosmetic and food industries. The polyunsaturated fatty acids (PUFAs) obtained from oleaginous microalgae show benefits over other PUFAs sources such as fish oils, being odorless, and non-dependent on fish stocks. Heterotrophic microalgae can use low-cost substrates such as organic wastes/residues containing carbon, simultaneously producing PUFAs together with other lipids that can be further converted into bioenergy, for combined heat and power (CHP), or liquid biofuels, to be integrated in the transportation system. This review analyses the different strategies that have been recently used to cultivate and further process heterotrophic microalgae for lipids, with emphasis on omega-3 rich compounds. It also highlights the importance of studying an integrated process approach based on the use of low-cost substrates associated to the microalgal biomass biorefinery, identifying the best sustainability methodology to be applied to the whole integrated system.

scholarly journals Probeless non-invasive near-infrared spectroscopic bioprocess monitoring using microspectrometer technology

2019 ◽  
Vol 412 (9) ◽  
pp. 2103-2109 ◽  
Author(s):  
Robert Zimmerleiter ◽  
Julian Kager ◽  
Ramin Nikzad-Langerodi ◽  
Vladimir Berezhinskiy ◽  
Frank Westad ◽  
...  
Keyword(s):  
Real Time ◽  
Process Parameters ◽  
Near Infrared ◽  
Low Cost ◽  
Phenoxyacetic Acid ◽  
Bioprocess Monitoring ◽  
Fed Batch ◽  
Precise Control ◽  
Fed Batch Fermentation ◽  
Using Data

AbstractReal-time measurements and adjustments of critical process parameters are essential for the precise control of fermentation processes and thus for increasing both quality and yield of the desired product. However, the measurement of some crucial process parameters such as biomass, product, and product precursor concentrations usually requires time-consuming offline laboratory analysis. In this work, we demonstrate the in-line monitoring of biomass, penicillin (PEN), and phenoxyacetic acid (POX) in a Penicilliumchrysogenum fed-batch fermentation process using low-cost microspectrometer technology operating in the near-infrared (NIR). In particular, NIR reflection spectra were taken directly through the glass wall of the bioreactor, which eliminates the need for an expensive NIR immersion probe. Furthermore, the risk of contaminations in the reactor is significantly reduced, as no direct contact with the investigated medium is required. NIR spectra were acquired using two sensor modules covering the spectral ranges 1350–1650 nm and 1550–1950 nm. Based on offline reference analytics, partial least squares (PLS) regression models were established for biomass, PEN, and POX either using data from both sensors separately or jointly. The established PLS models were tested on an independent validation fed-batch experiment. Root mean squared errors of prediction (RMSEP) were 1.61 g/L, 1.66 g/L, and 0.67 g/L for biomass, PEN, and POX, respectively, which can be considered an acceptable accuracy comparable with previously published results using standard process spectrometers with immersion probes. Altogether, the presented results underpin the potential of low-cost microspectrometer technology in real-time bioprocess monitoring applications.

scholarly journals CHORUME COMO FONTE DE NUTRIENTE NA PRODUÇÃO DA BIOMASSA MICROALGAL

e-xacta ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 11
Author(s):  
Najla Postaue ◽  
Leila Cristina Moraes ◽  
Rosa Maria Farias Asmus
Keyword(s):  
Fatty Acids ◽  
Culture Media ◽  
Biomass Conversion ◽  
Low Cost ◽  
Methyl Esters ◽  
Saturated Fatty Acids ◽  
Biodiesel Production ◽  
Microalgae Cultivation ◽  
Microalgal Biomass ◽  
Promising Source

A biomassa de microalgas tem apresentado potencial para produção de biodiesel, contudo a viabilidade do cultivo de microalgas depende de fonte de nutrientes de baixo custo. O presente estudo objetivou utilizar o chorume como fonte de nutrientes para microalgas. Os experimentos foram conduzidos visando avaliar a obtenção da biomassa microalgal, conversão de lipídios e rendimento em ésteres metílicos de ácidos graxos, para os meios de cultivos utilizando 5%, 12% e 20% de chorume, com concentrações de 0,02, 0,05 e 0,08 g N. L-1 e para meio de controle contendo 1% de, Nitrogênio (N), Fósforo (P) e Potássio (K), na concentração de 20 g L-1, 5 g L-1 e 20 g L-1, respectivamente. A microalga utilizada neste trabalho foi a de classe Chlorophyceae e família Coccomyxaceae. Os resultados demonstraram que o meio com concentração de 12% de chorume obteve melhores resultados, possibilitando alcançar 1,19 g de biomassa, conversão de 108,15 mg g-1 de lipídios e conteúdo de ésteres de 410,77mg g-1, a microalga utilizada apresentou ainda predominância dos ácidos graxos palmítico e oleico, apresentando baixa quantidade de ácidos graxos saturados o que pode fornecer ao combustível, resistência ao frio. E tais aspectos demonstraram que o chorume pode ser uma fonte promissora de nutrientes para o cultivo das microalgas estudadas. AbstractMicroalgae biomass has presented potential for biodiesel production, however the viability of microalgae cultivation depends on low cost nutrient source. The present study aimed to use leachate as a source of nutrients for microalgae. The experiments were conducted to evaluate the microalgal biomass, lipid conversion and yield in fatty acid methyl esters, for the culture media using 5%, 12% and 20% leachate, with concentrations of 0.02, 0.05 and 0.08 g N. L-1 and for control medium containing 1% Nitrogen (N), Phosphorus (P) and Potassium (K), at a concentration of 20 g L-1, 5 g L-1 and 20 g L-1, respectively. The microalgae used in this work was Chlorophyceae class and Coccomyxaceae family. The results showed that the medium with a concentration of 12% of leachate obtained better results, allowing to reach 1.19 g of biomass, conversion of 108.15 mg g-1 of lipids and esters content of 410,77 mg g-1. The microalgae used also presented predominance of palmitic and oleic fatty acids, presenting low amount of saturated fatty acids which can provide the fuel with cold resistance. And these aspects demonstrated that the leachate can be a promising source of nutrients for the cultivation of the studied microalgae.

scholarly journals Smartphone-Based Optical Fiber Sensor for the Assessment of a Fed-Batch Bioreactor

2020 ◽  
Vol 2 (1) ◽  
pp. 26
Author(s):  
Marco César Prado Soares ◽  
Thiago Destri Cabral ◽  
Pedro Machado Lazari ◽  
Matheus dos Santos Rodrigues ◽  
Gildo Santos Rodrigues ◽  
...  
Keyword(s):  
Optical Fiber ◽  
Optical Fibers ◽  
Sucrose Concentration ◽  
Low Cost ◽  
Optical Fiber Sensor ◽  
Refraction Index ◽  
Fed Batch ◽  
Pixel Intensity ◽  
Batch Bioreactor ◽  
Sensing Platform

Industry is currently in a period of great expansion, the so-called “Industry 4.0”. This period relies on the development of new sensor technologies for the generation of systems capable of collecting, distributing, and delivering information. Particularly in chemical and biochemical industries, the development of portable monitoring devices can improve many process parameters, such as safety and productivity. In this work, the design of a smartphone-based optical fiber sensing platform for the online assessment of fed-batch fermentation systems is reported. The setup is comprised of a smartphone equipped with a 3D-printed case that couples optical fibers to the phone, and of an application for collecting images from the camera and then analyzing the pixel intensity. Finally, the obtained intensities are correlated to the broth refraction index, which is function of the sucrose concentration. We calculated the sensitivity of this sensor as 85.83 RIU−1 (refractive index units), and then compared its performance to results obtained with a handheld refractometer and with Monod model predictions. It showed to be a reliable, portable, and low-cost instrument for the online monitoring of bioreactors that can be easily reproducible on-site by simply printing it.

The Open Culture of Nostoc flagelliforme with a 25 L Open Pond

2012 ◽  
Vol 554-556 ◽  
pp. 1009-1012 ◽  
Author(s):  
Ning Tan ◽  
Shi Ru Jia ◽  
Pei Pei Han ◽  
Wei Guo ◽  
Yu Jie Dai
Keyword(s):  
Mass Production ◽  
Culture System ◽  
Low Cost ◽  
Batch Cultivation ◽  
Alkali Resistance ◽  
Nutrient Elements ◽  
Fed Batch ◽  
Exopolysaccharide Production ◽  
Whole Process ◽  
Fed Batch Cultivation

The open culture is a simple, low-cost way for microalage cultivation, and the great advantage of which is that it can be easily used for mass production. This culture system is primarily used for the alga that can survive under bad or extreme conditions. N. flagelliforme is a kind autotrophic alga having the property of alkali-resistance, which makes open culture of N. flagelliforme become possible. In this study, the cultivation of N.flagelliforme with an open, 60×40×30cm rectangular glass reactor was conducted. Firstly, the open culture of N.flagelliforme was carried on without new nutrient elements supplemented into pond intermittently. During the whole process of cultivation, the pH maintained at 8-9 in order to avoid the contamination of other green alga. At the end of the cultivation, the cell density reached 0.802 g•L-1 and the exopolysaccharide concentration was 97mg•L-1. Secondly, the fed-batch cultivation was employed to increase biomass and exopolysaccharide production. At the end of the fed-batch cultivation, the biomass and exopolysaccharide of N.flagelliforme increased distinctly to 1.16 g•L-1 and 124 mg•L-1, respectively.

scholarly journals The Prospects of Agricultural and Food Residue Hydrolysates for Sustainable Production of Algal Products

Energies ◽  
2020 ◽  
Vol 13 (23) ◽  
pp. 6427
Author(s):  
Ehab M. Ammar ◽  
Neha Arora ◽  
George P. Philippidis
Keyword(s):  
Animal Feed ◽  
Low Cost ◽  
Industrial Applications ◽  
Sustainable Production ◽  
Cultivation Conditions ◽  
Microalgal Biomass ◽  
Food Residue ◽  
Algal Products ◽  
Sustainable Cultivation ◽  
Food Residues

The growing demand of microalgal biomass for biofuels, nutraceuticals, cosmetics, animal feed, and other bioproducts has created a strong interest in developing low-cost sustainable cultivation media and methods. Agricultural and food residues represent low-cost abundant and renewable sources of organic carbon that can be valorized for the cultivation of microalgae, while converting them from an environmental liability to an industrial asset. Biochemical treatment of such residues results in the release of various sugars, primarily glucose, sucrose, fructose, arabinose, and xylose along with other nutrients, such as trace elements. These sugars and nutrients can be metabolized in the absence of light (heterotrophic) or the presence of light (mixotrophic) by a variety of microalgae species for biomass and bioproduct production. The present review provides an up-to-date critical assessment of the prospects of various types of agricultural and food residues to serve as algae feedstocks and the microalgae species that can be grown on such residues under a range of cultivation conditions. Utilization of these feedstocks can create potential industrial applications for sustainable production of microalgal biomass and bioproducts.

scholarly journals Fed-batch carotenoid production by Phaffia rhodozyma Y-17268 using agroindustrial substrates

2020 ◽  
Vol 10 (3) ◽  
pp. 5348-5354
Keyword(s):  
Crude Glycerol ◽  
Low Cost ◽  
Corn Steep Liquor ◽  
Aeration Rate ◽  
Phaffia Rhodozyma ◽  
Agitation Rate ◽  
Fed Batch ◽  
Batch Bioreactor ◽  
Steep Liquor ◽  
Β Carotene

This work aim the carotenoid bioproduction by the yeast Phaffia rhodozyma Y-17268 in a fed-batch bioreactor with different low-cost agroindustrial substrates (crude glycerol, corn steep liquor, and rice parboiling water). The maximum concentration of total carotenoid and cell productivity were 4118 µg/L (835 µg/g) and 0.05 g/L. h, respectively, with a feed volume of 75 mL every 12 h. The medium were composed of 100 g/L crude glycerol, 100 g/L corn steep liquor, and 20 g/L rice parboiling water at 25ºC, pHinitial 4.0, agitation rate of 250 rpm, aeration rate of 1.5 vvm and 96 h of bioproduction. 0.188 h-1 of maximum specific growth speeds (μmax) was obtained for the major carotenoid - (all-E)-β-carotene (75.9%). Thus, the yeast P. rhodozyma produced in a fed-batch bioreactor demonstrated a great potential to produce the β-carotene.

scholarly journals An Innovative Low-Cost Equipment for Electro-Concentration of Microalgal Biomass

2020 ◽  
Author(s):  
Edwar M. Sanchez-Galvis ◽  
Ingri Y. Cardenas-Gutierrez ◽  
Jefferson E. Contreras-Ropero ◽  
Janet B. García-Martínez ◽  
Andrés F. Barajas-Solano ◽  
...  
Keyword(s):  
Research And Development ◽  
Low Cost ◽  
Laboratory Scale ◽  
Suitable Method ◽  
Innovative Approach ◽  
Mixing Rate ◽  
Microalgal Biomass ◽  
Biomass Quality ◽  
The World ◽  
Different Parts

Microalgal harvesting is one of the most challenging processes in the development of algal research and development. Several methods, such as centrifugation, flocculation, and filtration, are available at the laboratory scale. However, the requirement of expensive pieces of equipment and the possibility of biomass contamination are recurring gaps that hinder the development of microalgae I+D in different parts of the world. Recently, the electroflotation has been proved as a suitable method for the harvesting of different species of microalgae and cyanobacteria. To this day, there are no companies that sell laboratory-scale electroflotation equipment; this is mainly due to the gap in the knowledge on which factors (time, mixing rate, number of electrodes, and others) will affect the efficiency of concentration without reducing the biomass quality. This paper aims to build an innovative low-cost electroflotation system under 300 USD with cheap and resistant materials. To achieve our goal, we test the interaction of three variables (time, mixing rate, and amount of electrodes) were evaluated. Results showed that an efficiency closer to 100% could be achieved under 20 minutes using >10 electrodes and 150 rpm. We hope this innovative approach can be used by different researchers to improve our knowledge of the concentration and harvesting of algae and cyanobacteria.

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