Antinociceptive effect of Teucrium polium leaf extract in the diabetic rat formalin test

The aim of this study was to examine if the peripheral antinociception of α-bisabolol involve the participation of nitric oxide (NO) and cyclic guanosine monophosphate (cGMP) synthesis followed by K+ channel opening in the formalin test. Wistar rats were injected in the dorsal surface of the right hind paw with formalin (1%). Rats received a subcutaneous injection into the dorsal surface of the paw of vehicles or increasing doses of α-bisabolol (100-300 µg/paw). To determine whether the peripheral antinociception induced by α-bisabolol was mediated by either the opioid receptors or the NO-cGMP-K+ channels pathway, the effect of pretreatment (10 min before formalin injection) with the appropriate vehicles, naloxone, naltrexone, L-NAME, ODQ, glibenclamide, glipizide, apamin, charybdotoxin, tetraethylammonium or 4-aminopyridine on the antinociceptive effects induced by local peripheral α-bisabolol (300 µg/paw) were assessed. α-bisabolol produced antinociception during both phases of the formalin test. α-bisabolol antinociception was blocked by L-NAME, ODQ, and all the K+ channels blockers. The peripheral antinociceptive effect produced by α-bisabolol was not blocked by the opioid receptor inhibitors. α-bisabolol was able to active the NO-cGMP-K+ channels pathway in order to produce its antinoceptive effect. The participation of opioid receptors in the peripheral local antinociception induced by α-bisabolol is excluded.

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Ellagic Acid ameliorates Streptozotocin-Induced Diabetic Hyperalgesia in Rat: Involvement of Oxidative Stress

Basic and Clinical Neuroscience Journal ◽

10.32598/bcn.2021.2413.1 ◽

2021 ◽

Author(s):

Siamak Shahidi ◽

◽

Alireza Komaki ◽

Safoura Raoufi ◽

Iraj Salehi ◽

...

Keyword(s):

Oxidative Stress ◽

Ellagic Acid ◽

Therapeutic Potential ◽

Biological Activities ◽

Antinociceptive Effect ◽

Diabetic Rats ◽

Diabetic Rat ◽

Tail Flick ◽

Stress Markers ◽

Total Antioxidant

Background/Aim: Hyperalgesia is one of the current complications of diabetes mellitus that Oxidative stress and inflammation have principal role in its development. Ellagic Acid (EA) as a herbal component, has some biological activities, including antioxidant and anti-inflammatory effects. This study was designed to evaluate the possible beneficial effect of EA on hyperalgesia in streptozotocin (STZ)-induced diabetic rat. Materials and Methods: Rats were divided into control(vehicle received), diabetic, EA (25, 50 mg/kg)-treated control and EA(25, 50 mg/kg)-treated diabetic groups. Diabetes was induced by a single intraperitoneal (IP) injection of streptozotocin (STZ) (60 mg/Kg). EA was administered daily by oral gavage for 4 weeks. Hyperalgesia was assessed using tail flick (TF) and hot plate (HP) tests. Also, oxidative stress markers including malondialdehyde (MDA), total oxidant status (TOS) and total antioxidant capacity (TAC) in the serum were evaluated. Results: Diabetic animals showed marked reductions in TF and HP latencies, elevation of serum MDA level and TOS and diminution of serum TAC compared to controls significantly. Treatment of Diabetic rats with EA ameliorated reduction of TF latency at the dose of 25 mg/kg and HP latency at the dose of 50 mg/kg. Furthermore EA significantly increased TAC and decreased MDA level at dose of 50 mg/kg and reduced TOS at both doses in the serum of diabetic animals. In EA treated normal rats we could see no significant alterations in the parameters studied. Conclusion: These results displayed potent antinociceptive effect of EA in diabetic rats via attenuating oxidative stress. This proposes therapeutic potential of EA for damping diabetic hyperalgesia.

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Efektivitas Pandan (Pandanus Amarilifolius Roxb) Sebagai Pereduksi Alami Kadar Formalin pada Cincau Hitam

Journal of Pharmacy and Science ◽

10.53342/pharmasci.v4i2.142 ◽

2019 ◽

Vol 4 (2) ◽

pp. 91-95

Author(s):

Galuh Ratmana Hanum ◽

Syahrul Ardiansyah ◽

Puspita Handayani

Keyword(s):

Calcium Level ◽

Leaf Extract ◽

Formalin Test ◽

Statistical Data ◽

Soaking Time ◽

Air Content ◽

Extract Concentration ◽

Saponin Content ◽

Harmful Effects ◽

Pandanus Amaryllifolius

ABSTRAKCincau Hitam merupakan salah satu makanan yang memiliki daya simpan yang pendek, maka penambahan formalin sering digunakan supaya daya simpan cincau hitam semakin lama. Formalin dapat menimbulkan efek berbahaya bagi kesehatan. Salah satu cara untuk menurunkan kadar formalin pada makanan yaitu menggunakan daun pandan (Pandanus amarillifolius Roxb.) yang memiliki kandungan saponin. Tujuan penelitian ini yaitu untuk mengetahui konsentrasi dan lama perendaman terbaik sari daun pandan dalam menurunkan kadar formalin. Sampel yang digunakan yaitu sampel cincau hitam yang selanjutnya direndam dengan variasi konsentrasi sari daun pandan 15%, 20% dan 25% selama 15, 30, 45 dan 60 menit. Parameter yang digunakan yaitu uji formalin secara kualitatif, uji formalin secara kuantitatif, kadar air dengan metode gravimetri, kadar karbohidrat dan uji kalsium, data diuji statistika menggunakan Two Way Anova. Hasil penelitian ini konsentrasi dan lama perendaman terbaik sari daun pandan (Pandanus amaryllifolius Roxb.) pada cincau hitam terhadap penurunan kadar formalin yaitu 25% dengan perendaman 60 menit.Kata kunci: Cincau hitam, Sari daun pandan (Pandanus amarillifolius Roxb.), Formalin, Kadar Formalin.ABSTRACTBlack grass jelly is one of the foods that has a short shelf life, so formaldehyde is often used to save black grass jelly power for longer time. Formalin can have harmful effects on health. One way to reduce formalin levels in foods is to use pandan leaves (Pandanus amarillifolius Roxb.) Which has saponin content. The purpose of this study was to study the concentration and soaking time of the best pandan leaf extract to reduce formalin levels. The samples used were samples of black grass jelly which were then soaked with variations in pandan leaf extract concentration of 15%, 20% and 25% for 15, 30, 45 and 60 minutes. The parameters used were qualitative formalin test, quantitative formalin test, air content, calcium level and calcium test, statistical data collected using Two Way Anova. The results of this study were the best concentration and soaking time of pandan leaf extract (Pandanus amaryllifolius Roxb.) In black grass against a decrease in formalin levels of 25% with 60 minutes soaking.Keywords: Black grass jelly, pandan leaf extract (Pandanus amarillifolius Roxb.), Formalin, formalin levels.

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Skrining Mekanisme Kerja Daun Malaka (Phyllanthus emblica L.) Sebagai Antidiabetes

Talenta Conference Series Tropical Medicine (TM) ◽

10.32734/tm.v1i3.271 ◽

2018 ◽

Vol 1 (3) ◽

pp. 106-110

Author(s):

Novi Irwan Fauzi ◽

Seno Aulia Ardiansyah ◽

Saeful Hidayat

Keyword(s):

Mechanism Of Action ◽

Inhibitory Activity ◽

Leaf Extract ◽

Test Method ◽

Diabetic Rat ◽

Phyllanthus Emblica ◽

Insulin Sensitizer ◽

Water Fraction

Daun malaka (Phyllanthus emblica L.) mempunyai potensi digunakan sebagai alternatif obat antidiabetes. Daun malaka menunjukkan efek hipoglikemia pada tikus yang diinduksi aloksan. Namun, mekanisme kerjanya belum diketahui pasti. Penelitian ini dilakukan dalam rangka skrining mekanisme kerja daun malaka sebagai antidiabetes. Skrining mekanisme kerja dilakukan terhadap fraksi air daun malaka melalui uji aktivitas inhibisi enzim α-glukosidase serta α-amilase secara in vitro dan pengujian aktivitas insulin-sensitizer terhadap ekstrak daun malaka dengan metode tes toleransi insulin secara in vivo. Fraksi air daun malaka menunjukkan aktivitas inhibisi terhadap enzim α-glukosidase serta α-amilase dengan nilai IC50 (Inhibitor Concentration 50) pada kedua enzim tersebut berturut-turut adalah 0,87% dan 8,64% b/v. Pada uji aktivitas insulin sensitizer, pemberian ekstrak daun malaka dapat meningkatkan sensitivitas insulin pada tikus diabet dengan kondisi resistensi insulin. Nilai KTTI pada kelompok tikus diabet yang diberi ekstrak daun malaka dosis 100 dan 500 mg/kgbb tikus (74,89 dan 75,57) lebih tinggi dibandingkan kelompok tikus diabet (38,41) dan kadar glukosa darah yang lebih rendah selama interval waktu pengukuran. Daun malaka telah diketahui mampu meningkatkan sekresi insulin dan pada penelitian ini menunjukkan aktivitas inhibisi enzim α-glukosidase serta α-amilase secara in vitro dan menunjukkan aktivitas insulinsensitizer pada tikus diabet dengan kondisi resistensi insulin. Malaka leaf (Phyllanthus emblica L.) has the potential to be used as an alternative antidiabetic drug. Malacca leaves showed hypoglycemia effect in rat induced by alloxan. However, the mechanism of action is not yet known. This study was conducted to evaluate the mechanism of action of Malaka leaves as antidiabetic. Screening of the mechanism of action was carried out on the water fraction of Malaka leaf byinhibitory activity examination on α-glucosidase and α-amylase by in vitro studyand Evaluation of insulin-sensitizer activity of Maaka leaf leaf extract was conducted by invivo insulin tolerance test method. Malaka leaf water fraction showed inhibitory activity against the α-glucosidase and α-amylase with IC50 values (Inhibitory Concentration 50) of0.87% and 8.64% b / v on both enzyme, respectively. The evaluation of insulin sensitizer revelead that administration ofMalaka leaf extract can increase insulin sensitivity in diabetic rat with insulin resistance.KTTI values in diabetic rats given malaka extract at the dose of 100 and 500 mg / kg BW (74.89 and 75.57) were higher than diabetics rat (38.41) and the extract also decrease blood glucose levels during measurement time intervals . Malaka leafhas been known to increase insulin secretion and the study showedthe inhibitory activity on α-glucosidase and α-amylase by in vitro study and showed insulinsensitizer activity in diabetic rat with insulin resistance.

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