In vitro anti-glycation activity of the marine plant Posidonia oceanica (L.) Delile

2020 ◽  
Vol 259 ◽  
pp. 112960 ◽  
Author(s):  
Marzia Vasarri ◽  
Emanuela Barletta ◽  
Matteo Ramazzotti ◽  
Donatella Degl’Innocenti
Marine Drugs ◽  
2021 ◽  
Vol 19 (2) ◽  
pp. 48
Author(s):  
Laura Micheli ◽  
Marzia Vasarri ◽  
Emanuela Barletta ◽  
Elena Lucarini ◽  
Carla Ghelardini ◽  
...  

Posidonia oceanica (L.) Delile is traditionally used for its beneficial properties. Recently, promising antioxidant and anti-inflammatory biological properties emerged through studying the in vitro activity of the ethanolic leaves extract (POE). The present study aims to investigate the anti-inflammatory and analgesic role of POE in mice. Inflammatory pain was modeled in CD-1 mice by the intraplantar injection of carrageenan, interleukin IL-1β and formalin. Pain threshold was measured by von Frey and paw pressure tests. Nociceptive pain was studied by the hot-plate test. POE (10–100 mg kg−1) was administered per os. The paw soft tissue of carrageenan-treated animals was analyzed to measure anti-inflammatory and antioxidant effects. POE exerted a dose-dependent, acute anti-inflammatory effect able to counteract carrageenan-induced pain and paw oedema. Similar anti-hyperalgesic and anti-allodynic results were obtained when inflammation was induced by IL-1β. In the formalin test, the pre-treatment with POE significantly reduced the nocifensive behavior. Moreover, POE was able to evoke an analgesic effect in naïve animals. Ex vivo, POE reduced the myeloperoxidase activity as well as TNF-α and IL-1β levels; further antioxidant properties were highlighted as a reduction in NO concentration. POE is the candidate for a new valid strategy against inflammation and pain.


PROTEOMICS ◽  
2015 ◽  
Vol 15 (23-24) ◽  
pp. 4159-4174 ◽  
Author(s):  
Amalia Piro ◽  
Ilia Anna Serra ◽  
Antonia Spadafora ◽  
Monica Cardilio ◽  
Linda Bianco ◽  
...  

2020 ◽  
Vol 247 ◽  
pp. 112252 ◽  
Author(s):  
Marzia Vasarri ◽  
Manuela Leri ◽  
Emanuela Barletta ◽  
Matteo Ramazzotti ◽  
Riccardo Marzocchini ◽  
...  

Marine Drugs ◽  
2021 ◽  
Vol 19 (9) ◽  
pp. 476 ◽  
Author(s):  
Marzia Vasarri ◽  
Anna Maria De Biasi ◽  
Emanuela Barletta ◽  
Carlo Pretti ◽  
Donatella Degl’Innocenti

Posidonia oceanica (L.) Delile is a Mediterranean-endemic angiosperm often described for its great ecological importance. Despite evidence of a millennia-old relationship between P. oceanica and humans, as well as traditional medicine applications, the potential benefits of P. oceanica for human health have been documented only recently. This review aims to compile newly acquired knowledge on P. oceanica bioactive properties that allow the scientific community to look at this plant as a promising source of natural therapeutical products for human health. Experimental investigations conducted in both in vitro cellular-based and in vivo animal models pave the way for new research projects aiming at the development of alternative and complementary therapeutic strategies based on P. oceanica against a wide range of pathological conditions.


1998 ◽  
Vol 60 (1) ◽  
pp. 83-93 ◽  
Author(s):  
Elena Balestri ◽  
Luigi Piazzi ◽  
Francesco Cinelli

Marine Drugs ◽  
2021 ◽  
Vol 19 (10) ◽  
pp. 560
Author(s):  
Concetta Maria Messina ◽  
Rosaria Arena ◽  
Simona Manuguerra ◽  
Yann Pericot ◽  
Eleonora Curcuraci ◽  
...  

The marine environment is a generous source of biologically active compounds useful for human health. In 50 years, about 25,000 bioactive marine compounds have been identified, with an increase of 5% per year. Peculiar feature of algae and plants is the production of secondary metabolites, such as polyphenols, synthesized as a form of adaptation to environmental stress. Posidonia oceanica is a Mediterranean endemic and dominant seagrass and represents a biologically, ecologically and geologically important marine ecosystem. Within this study, methanolic and ethanolic extracts were generated from fresh and dried Posidonia oceanica leaves, with the aim to employ and valorize the beach cast leaves. The best yield and antioxidant activity (polyphenols content equal to 19.712 ± 0.496 mg GAE/g and DPPH IC50 of 0.090 µg/µL.) were recorded in 70% ethanol extracts (Gd-E4) obtained from leaves dried for two days at 60 °C and ground four times. HPLC analyses revealed the presence of polyphenols compounds (the most abundant of which was chicoric acid) with antioxidant and beneficial properties. Bioactive properties of the Gd-E4 extracts were evaluated in vitro using fibroblast cells line (HS-68), subjected to UV induced oxidative stress. Pre-treatment of cells with Gd-E4 extracts led to significant protection against oxidative stress and mortality associated with UV exposure, thus highlighting the beneficial properties of antioxidants compounds produced by these marine plants against photo damage, free radicals and associated negative cellular effects. Beach cast leaves selection, processing and extraction procedures, and the in vitro assay results suggested the potentiality of a sustainable approach for the biotechnological exploitation of this resource and could serve a model for other marine resources.


2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
S. Kouki ◽  
N. Saidi ◽  
A. Ben Rajeb ◽  
M. Brahmi ◽  
A. Bellila ◽  
...  

A compost of vegetable waste andPosidonia oceanicamixture (70 : 30% vol : vol) was testedin vitroandin vivofor its efficacy againstFusarium oxysporumf.spradicis-lycopersici(Forl), the causal agent of Fusarium wilt of Tomato (Lycopersicon esculentumcv. chourouk).The incorporation of non-sterilized VPC in the culture medium showed potent antifungal activity against Forl and complete inhibition of mycelium growth was observed for all the tested compost rates (0.5, 1, 2, 4, 6, 8, 10, 15 and 20%). However, only the highest rates (15 and 20%) of a sterilized suspension of VPC were effective in preventing mycelial growth. Nine indigenous bacterial strains isolated from VPC exhibited antagonism against Forl. Based on 16S rDNA sequence analysis, the isolates were assigned toBacillus sphaericus(B12 and BS2),Pseudomonas putidaPPS7 andBurkholderia gladioliBuC16. Under green house condition, seed inoculation by B12, BS2, PP7 and BuC16 strains protected significantly tomato againstFusarium oxysporumf.spradicis-lycopersici(Forl) attacks.


2011 ◽  
Vol 10 (16) ◽  
pp. 3176-3185 ◽  
Author(s):  
Kesraoui Ons ◽  
Nejib Marzouki Mohamed ◽  
Maugard Thierry ◽  
Limam Ferid

Author(s):  
P.L. Moore

Previous freeze fracture results on the intact giant, amoeba Chaos carolinensis indicated the presence of a fibrillar arrangement of filaments within the cytoplasm. A complete interpretation of the three dimensional ultrastructure of these structures, and their possible role in amoeboid movement was not possible, since comparable results could not be obtained with conventional fixation of intact amoebae. Progress in interpreting the freeze fracture images of amoebae required a more thorough understanding of the different types of filaments present in amoebae, and of the ways in which they could be organized while remaining functional.The recent development of a calcium sensitive, demembranated, amoeboid model of Chaos carolinensis has made it possible to achieve a better understanding of such functional arrangements of amoeboid filaments. In these models the motility of demembranated cytoplasm can be controlled in vitro, and the chemical conditions necessary for contractility, and cytoplasmic streaming can be investigated. It is clear from these studies that “fibrils” exist in amoeboid models, and that they are capable of contracting along their length under conditions similar to those which cause contraction in vertebrate muscles.


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