Anti-tumor and immunomodulatory effects of Grifola frondosa polysaccharide combined with vitamin C on Heps-bearing mice: Based on inducing apoptosis and autophagy

The present work was designed to study the antidiabetic and immunomodulatory effects of olive leaf polyphenol (Oleuropein) and vitamin C in diabetic male rats. Diabetes was induced by a single i.p. dose of STZ (40 mg/kg b.w.). Pure oleuropein compound (5 mg/kg b.w.) and vit. C (150 mg/kg b.w.) were orally administered once per a day for 15 days after diabetes induction. Oleuropein and vitamin C showed a significant role in attenuating the blood glucose, insulin, pancreatic amylase, and hexokinase alterations and caused an elevation of antioxidant enzymes in diabetic rats. Oleuropein and vitamin C showed a hematopoetic action as documented by the increase in RBC, Hb, Hct, PLt, decrease in WBC and improved RBC structure. Oleuropein and vitamin C significantly attenuated the oxidative status of diabetic rats. Oleuropein and vitamin C improved the different changes in some cytokines and interlukines and decreases the gene expression of TNF-, COX2, and caspase3 involved in apoptosis and inflammation. The immunohistochemical sections of pancreas in the diabetic rats treated with oleuropein and vitamin C showed a high intensity of insulin in -cells. Oleuropein as a natural active compound has antioxidant activity more or less like vitamin C to attenuate the effect of STZ-induced diabetes.

Download Full-text

Feasibility of Vitamin C in the Treatment of Post Viral Fatigue with Focus on Long COVID, Based on a Systematic Review of IV Vitamin C on Fatigue

Nutrients ◽

10.3390/nu13041154 ◽

2021 ◽

Vol 13 (4) ◽

pp. 1154

Author(s):

Claudia Vollbracht ◽

Karin Kraft

Keyword(s):

Vitamin C ◽

Sleep Disturbances ◽

Positive Impact ◽

Treatment Options ◽

Present Knowledge ◽

Control Group ◽

High Dose ◽

Immunomodulatory Effects ◽

Before And After ◽

Suitable Treatment

Fatigue is common not only in cancer patients but also after viral and other infections. Effective treatment options are still very rare. Therefore, the present knowledge on the pathophysiology of fatigue and the potential positive impact of treatment with vitamin C is illustrated. Additionally, the effectiveness of high-dose IV vitamin C in fatigue resulting from various diseases was assessed by a systematic literature review in order to assess the feasibility of vitamin C in post-viral, especially in long COVID, fatigue. Nine clinical studies with 720 participants were identified. Three of the four controlled trials observed a significant decrease in fatigue scores in the vitamin C group compared to the control group. Four of the five observational or before-and-after studies observed a significant reduction in pre–post levels of fatigue. Attendant symptoms of fatigue such as sleep disturbances, lack of concentration, depression, and pain were also frequently alleviated. Oxidative stress, inflammation, and circulatory disorders, which are important contributors to fatigue, are also discussed in long COVID fatigue. Thus, the antioxidant, anti-inflammatory, endothelial-restoring, and immunomodulatory effects of high-dose IV vitamin C might be a suitable treatment option.

Download Full-text

Immunomodulatory Effects of Dietary β-glucan and Vitamin C in Nile Tilapia,Oreochromis niloticusL., Subjected to Cold-induced Stress or Bacterial Challenge

Journal of the World Aquaculture Society ◽

10.1111/jwas.12202 ◽

2015 ◽

Vol 46 (4) ◽

pp. 363-380 ◽

Cited By ~ 6

Author(s):

Margarida M. Barros ◽

Dario R. Falcon ◽

Ricardo O. Orsi ◽

Luiz E. Pezzato ◽

Ademir C. Fernandes Junior ◽

...

Keyword(s):

Vitamin C ◽

Nile Tilapia ◽

Bacterial Challenge ◽

Immunomodulatory Effects ◽

Induced Stress

Download Full-text

Synergistic Apoptotic Effect of D-Fraction From Grifola frondosa and Vitamin C on Hepatocellular Carcinoma SMMC-7721 Cells

Integrative Cancer Therapies ◽

10.1177/1534735416644674 ◽

2016 ◽

Vol 16 (2) ◽

pp. 205-214 ◽

Cited By ~ 5

Author(s):

Fei Zhao ◽

Yong-Feng Wang ◽

Lei Song ◽

Jia-Xin Jin ◽

Ya-Qing Zhang ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Cell Cycle ◽

Vitamin C ◽

Anticancer Activity ◽

Morphological Changes ◽

Grifola Frondosa ◽

Apoptotic Effect ◽

Anticancer Mechanism ◽

Induced Apoptosis

The aim of this study was to investigate the anticancer effect of a combination of D-fraction polysaccharide from Grifola frondosa (DFP) and vitamin C (VC) on hepatocellular carcinoma in vitro. DFP is a bioactive extract from the maitake mushroom. Anticancer activity was demonstrated using various concentrations of DFP alone or in combination with VC against the human hepatocarcinoma SMMC-7721 cell line. To investigate the anticancer mechanism, studies designed to detect cell apoptosis were conducted. Results from the MTT assay indicated that a combination of DFP (0.2 mg/mL) and VC (0.3 mmol/L) led to a 70% reduction in cell viability. Flow cytometry results indicated that DFP/VC treatment induced apoptosis in approximately 65% SMMC-7721 cells. Cell cycle analysis identified cell cycle arrest at the G2/M phase following DFP/VC treatment for 48 hours. In addition, cellular morphological changes were observed using transmission electron microscopy. Western blot analysis revealed that the upregulation of BAX, downregulation of Bcl-2, activation of poly-(ADP-ribose)-polymerase (PARP), and the release of cytochrome c were observed in cells treated with the combination of DFP/VC, which showed that the mechanism of anticancer activity in the SMMC-7721 hepatocarcinoma cells involved induction of apoptosis.

Download Full-text