Development of a new mathematical model for inactivation of Escherichia coli O157:H7 and Staphylococcus aureus by high hydrostatic pressure in carrot juice and peptone water

2009 ◽  
Vol 90 (3) ◽  
pp. 388-394 ◽  
Author(s):  
Mutlu Pilavtepe-Çelik ◽  
Sencer Buzrul ◽  
Hami Alpas ◽  
Faruk Bozoğlu
Keyword(s):  
Escherichia Coli ◽  
Mathematical Model ◽  
Staphylococcus Aureus ◽  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Escherichia Coli O157 ◽  
Carrot Juice ◽  
Peptone Water ◽  
And Staphylococcus Aureus

Comparison of Four Commercial DNA Extraction Kits for PCR Detection of Listeria monocytogenes, Salmonella, Escherichia coli O157:H7, and Staphylococcus aureus in Fresh, Minimally Processed Vegetables

2008 ◽  
Vol 71 (10) ◽  
pp. 2110-2114 ◽  
Author(s):  
P. ELIZAQUÍVEL ◽  
R. AZNAR
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Listeria Monocytogenes ◽  
Dna Extraction ◽  
Pcr Detection ◽  
Escherichia Coli O157 ◽  
Minimally Processed ◽  
Green Pepper ◽  
Minimally Processed Vegetables ◽  
And Staphylococcus Aureus

Four commercial DNA extraction methods, PrepMan Ultra (Applied Biosystems), InstaGene Matrix (BioRad), DNeasy Tissue kit (Qiagen), and UltraClean (MoBio), were tested for PCR detection of Listeria monocytogenes, Escherichia coli O157: H7, Salmonella, and Staphylococcus aureus in fresh, minimally processed vegetables. For comparative purposes, sensitivity assays with specific PCRs were carried out after DNA extraction with the four methods in green pepper, broccoli, and onion artificially inoculated with the four pathogens separately. As confirmed by statistical analysis, the DNeasy Tissue kit rendered the highest sensitivity values in the three matrices assayed for Salmonella, L. monocytogenes, and E. coli O157:H7 and in onion for S. aureus. Despite being the most expensive of the methods compared, the DNeasy Tissue Kit can be successfully applied for any of the four most commonly studied pathogens, thus saving time and overall reducing the cost of the analysis.

scholarly journals Optimized antibacterial measures against Escherichia coli O157:H7 and Staphylococcus aureus

2011 ◽  
Vol 5 (20) ◽  
pp. 3113-3121 ◽  
Author(s):  
Raftari ◽  
M ◽  
Jalilian F Azizi ◽  
Abdulamir ◽  
S A ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Escherichia Coli O157 ◽  
And Staphylococcus Aureus

Modeling transfer of Escherichia coli O157:H7 and Staphylococcus aureus during slicing of a cooked meat product

Meat Science ◽  
2007 ◽  
Vol 76 (4) ◽  
pp. 692-699 ◽  
Author(s):  
F. Pérez-Rodríguez ◽  
A. Valero ◽  
E.C.D. Todd ◽  
E. Carrasco ◽  
R.M. García-Gimeno ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Meat Product ◽  
Escherichia Coli O157 ◽  
Cooked Meat ◽  
And Staphylococcus Aureus
Sign in / Sign up

Export Citation Format

Share Document