In vitro screening of durum wheat against water-stress mediated through polyethylene glycol

Water stress caused by drought affects the productivity of the sugarcane crop. A breeding alternative is the selection of drought-tolerant sugarcane cultivars. The objective of this study was the in vitro screening of cultivars tolerant to water stress using polyethylene glycol (PEG) as a stressing agent. Cultivars (cv) Mex 69-290, CP 72-2086, Mex 79-431 and MOTZMex 92-207 were subjected to different concentrations of PEG 6000 (0, 3, 6 and 9% w/v) using Murashige and Skoog semi-solid culture medium. At 30 days of culture, different developmental variables and dry matter (DM), total protein (TP), proline (Pr) and glycine-betaine (GB) contents were evaluated. The results showed reduced development in cv CP 72-2086, Mex 79-431 and MOTZMex 92-207 with increasing PEG concentration. The cv Mex 69-290 showed tolerance to osmotic stress of −0.45 MPa using 3% PEG. Overall, TP content decreased with increasing PEG concentration, while DM, Pr and GB contents rose with increasing PEG concentration in all evaluated cultivars. Our results suggest that cv Mex 69-290 has a slight tolerance to water stress and could be used for rainfed cultivation with low rainfall or reduced irrigation for better water use efficiency. In conclusion, the in vitro screening technique of cultivars tolerant to PEG-induced water stress is an alternative for early determination of drought stress in sugarcane.

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Effect of water stress induced by polyethylene glycol 6000 on the somatic embryos conversion into whole plantlets in cocoa (Theobroma cacao L.)

International Journal of Biological and Chemical Sciences ◽

10.4314/ijbcs.v15i1.2 ◽

2021 ◽

Vol 15 (1) ◽

pp. 12-25

Author(s):

Tokpapon Eliane Manlé ◽

Kan Modeste Kouassi ◽

Brahima André Soumahoro ◽

Tchoa Koné ◽

Kouablan Edmond Koffi ◽

...

Keyword(s):

Climate Change ◽

Polyethylene Glycol ◽

Water Stress ◽

Somatic Embryos ◽

Stem Length ◽

Improvement Program ◽

Polyethylene Glycol 6000 ◽

Regenerated Plantlets ◽

Theobroma Cacao L

Rainfall scarcity due to climate change is a major constraint that limits cocoa productivity in Côte d'Ivoire. This work aims to regenerate cocoa plants tolerant to water stress using in vitro methods. Staminode and petal explants of the genotypes C1, C9, C14, C15, C16, C18 and C20 were used to produce somatic embryos through two methods. Firstly, somatic embryos were induced under stressfull conditions on media containing different concentrations of polyethylene glycol (PEG) 6000 (0; 25; 50; 75; 100 and 125 g/l) and secondly; under non-stressed conditions. Somatic embryos were placed on a conversion medium in the same stress condition. The number of regenerants decreased with the increase in the concentration of PEG with all genotypes. Only genotypes C1 and C15 regenerated plantlets under water stress conditions. The sensitive genotypes C9, C14, C16, C18 and C20 have not developed plantlets on media containing PEG. The plantlets produced under water deficit conditions exhibited a reduction in stem length and leaves number and an increase in length or offset of the high number of roots. The survival rate of regenerants during acclimatization was higher on the sandsubstrate. The selected genotypes could be used in an improvement program of cocoa production.Keywords: Climate change; plant regeneration; genotype; tolerance; drought; in vitro

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Recurrent Selection for Improved Germination under Water Stress in Russian Dandelion

Journal of the American Society for Horticultural Science ◽

10.21273/jashs03941-16 ◽

2017 ◽

Vol 142 (2) ◽

pp. 85-91 ◽

Cited By ~ 2

Author(s):

Katrina J.M. Hodgson-Kratky ◽

Olivier M. Stoffyn ◽

David J. Wolyn

Keyword(s):

Polyethylene Glycol ◽

Water Stress ◽

Recurrent Selection ◽

Field Experiments ◽

Phenotypic Selection ◽

Bare Soil ◽

Promising Candidate ◽

Breeding Programs ◽

Selection For

Russian dandelion [Taraxacum kok-saghyz (TKS)] is a promising candidate for introducing natural rubber production into North America. Seeds normally germinate in a humid microenvironment, such as the thatch layer of a lawn or under a canopy of grass; however, 5% to 15% establishment is often observed on bare soil, presumably due to water stress. Phenotypic selection and half-sib family recurrent selection were conducted for three cycles to improve germination in vitro, under low osmotic potential (Ψs), using a polyethylene glycol (PEG) solution. Populations were then tested for establishment on bare soil in the greenhouse and field. Germination under water stress in vitro increased from 5.8% for the cycle 0 (C0) population to 40.8% and 47.8% for the C3-phenotypic and C3-half-sib family populations, respectively. Soil establishment in the greenhouse and field was improved up to two- and 4-fold, respectively, compared with the C0, in two of four greenhouse experiments and three of eight field experiments. Overall, recurrent selection for germination under water stress in vitro has potential to improve establishment in the field and can be incorporated into current breeding programs to support the overall goal of creating cultivars with high-rubber yield.

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Effect of polyethylene glycol (PEG) 6000 on germination and seedling growth of pearl millet [Pennisetum glaucum (L.) R. Br.] and LD50 for in vitro screening for drought tolerance

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb2013.13514 ◽

2014 ◽

Vol 13 (37) ◽

pp. 3742-3747

Author(s):

OUSMANE Sani DAOUDA ◽

MOUNKAILA Boureima MOUHAMADOU

Keyword(s):

Polyethylene Glycol ◽

Drought Tolerance ◽

Pearl Millet ◽

Seedling Growth ◽

Pennisetum Glaucum ◽

In Vitro Screening ◽

Peg 6000

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The Growth Response of Several Potato Genotypes (Solanum tuberosum L.) to Induced Water Stress Using Sorbitol and Polyethylene Glycol

Notulae Scientia Biologicae ◽

10.15835/nsb849920 ◽

2016 ◽

Vol 8 (4) ◽

pp. 511-519 ◽

Cited By ~ 1

Author(s):

Mihaela A. CIOLOCA ◽

Andreea M. TICAN ◽

Maria IANOŞI ◽

Carmen L. BĂDĂRĂU

Keyword(s):

Polyethylene Glycol ◽

Water Stress ◽

Drought Tolerance ◽

Growth Response ◽

Culture Medium ◽

Growth Parameters ◽

The Other ◽

Addition Lines ◽

In Vitro Response

The current paper aimed to study the in vitro response of potato genotypes to water stress induced by adding sorbitol and polyethylene glycol in the culture medium. The biological material analysed in the experiment was represented by a Romanian line ‘LP 11-1525/1’ and two isogenic lines ‘LI 101’ and ‘LI 102’. For cultures initiation, the line ‘LP 11-1525/1’ was started from meristems and for the other two genotypes true potato seeds were used. The studied potato genotypes behaved differently depending on the analysed parameters and on the treatment applied for drought tolerance. It was noted that the line ‘LP 11-1525/1’ achieved good results for most of the growth parameters studied, and also the lines derived from true potato seeds behaved well, in some cases even exceeding the line derived from meristems. Of the lines derived from true potato seeds, the best performance was noted for line ‘LI 101-6’ in all the analysed parameters, both on sorbitol and PEG medium. In addition, lines ‘LI 101-7’ and ‘LI 102-4’ achieved good results on both variants of medium used to mediate water stress. Therefore, establishing drought tolerance individuals within populations derived from true potato seeds using sorbitol and polyethylene glycol might be applied.

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Simulation of in vitro water deficit for selecting drought-tolerant banana genotypes

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2017001200021 ◽

2017 ◽

Vol 52 (12) ◽

pp. 1301-1304 ◽

Cited By ~ 3

Author(s):

Alice Lichs Marssaro ◽

Lucymeire Souza Morais-Lino ◽

Jailson Lopes Cruz ◽

Carlos Alberto da Silva Ledo ◽

Janay Almeida dos Santos-Serejo

Keyword(s):

Polyethylene Glycol ◽

Water Stress ◽

Liquid Medium ◽

Water Deficit ◽

Dry Matter ◽

Stability Index ◽

Yield Stability ◽

Musa Sp ◽

Drought Tolerant

Abstract: The objective of this work was to determine a method to simulate water stress in vitro to select drought-tolerant banana (Musa sp.) genotypes. The BRS Tropical and Prata Anã cultivars were grown in vitro in an MS liquid medium containing sucrose and benzylaminopurine (BAP), supplemented with different concentrations of polyethylene glycol (PEG) or sorbitol. The yield stability index of dry matter was evaluated. Cultivation for 30 days in a medium containing 15 g L-1 PEG or 36.4 g L-1 sorbitol is appropriate to simulate water stress in vitro.

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