Pectinase-treated Panax ginseng protects against chronic intermittent heat stress-induced testicular damage by modulating hormonal and spermatogenesis-related molecular expression in rats

Testicular hyperthermia is well studied to cause impaired spermatogenesis. In the present study, the protective effect of enzymatically modified (pectinase-treated)Panax ginseng(GINST) against intermittent sub-chronic heat stress-induced testicular damage in rats was investigated. Male Sprague–Dawley rats were divided into four groups: normal control (NC), heat-stressed control (HC), heat-stressed plus GINST-100 mg/kg/day (HG100) and heat-stressed plus GINST-200 mg/kg/day (HG200) treatment groups. GINST (100 and 200 mg/kg/day) was mixed separately with a regular pellet diet and was administered orally for 8 weeks starting from 1 week before heat exposure. Parameters such as organ weight, blood chemistry, sperm kinetic values, expression of antioxidant enzymes, spermatogenesis molecules and sex hormone receptors levels were measured. Data revealed that kidney and epididymis weight were significantly (P < 0.05) decreased with heat stress and recovered by GINST treatment. Further, the altered levels of blood chemistry panels and sperm kinetic values in heat stress-induced rats were attenuated when GINST was administered (P < 0.05). Furthermore, the expression levels of antioxidant-related enzymes (GSTM5 and GPX4), spermatogenesis-related proteins (CREB1 and INHA) and sex hormone receptors (androgen receptor, luteinizing hormone receptor and follicle-stimulating hormone receptor) were reduced by heat stress; however, GINST treatment effectively ameliorated these changes. In conclusion, GINST was effective in reducing heat-induced damage in various male fertility factorsin vivoand has considerable potential to be developed as a useful supplement in improving male fertility.

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Korean Red Ginseng (Panax ginseng Meyer) with enriched Rg3 ameliorates chronic intermittent heat stress–induced testicular damage in rats via multifunctional approach

Journal of Ginseng Research ◽

10.1016/j.jgr.2018.06.004 ◽

2019 ◽

Vol 43 (1) ◽

pp. 135-142 ◽

Cited By ~ 5

Author(s):

Spandana Rajendra Kopalli ◽

Kyu-Min Cha ◽

Seock-Yeon Hwang ◽

Min-Sik Jeong ◽

Si-Kwan Kim

Keyword(s):

Heat Stress ◽

Panax Ginseng ◽

Red Ginseng ◽

Testicular Damage ◽

Korean Red Ginseng

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Long-Term Heat Stress Exposure Induced Poor Semen Parameters and Testicular Damage in Rats by Increasing Oxidative Stress, Sex Hormone Silencing, and Activation of Intrinsic Testicular Apoptosis [348]

Obstetrics and Gynecology ◽

10.1097/01.aog.0000463618.77648.44 ◽

2015 ◽

Vol 125 ◽

pp. 109S-110S

Author(s):

Mesfer Safar Al-Shahrani

Keyword(s):

Oxidative Stress ◽

Heat Stress ◽

Sex Hormone ◽

Semen Parameters ◽

Stress Exposure ◽

Testicular Damage

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Panax ginseng protects the testis against 2,3,7,8-tetrachlorodibenzo-p-dioxin induced testicular damage in guinea pigs

BJU International ◽

10.1046/j.1464-410x.1999.00046.x ◽

2001 ◽

Vol 83 (7) ◽

pp. 842-849 ◽

Cited By ~ 26

Author(s):

Kim ◽

Hwang ◽

Lee ◽

Song ◽

Kim

Keyword(s):

Panax Ginseng ◽

Guinea Pigs ◽

Testicular Damage ◽

Tetrachlorodibenzo P Dioxin

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Tender coconut water attenuates heat stress-induced testicular damage through modulation of the NF-κB and Nrf2 pathways

Food & Function ◽

10.1039/c8fo01207e ◽

2018 ◽

Vol 9 (10) ◽

pp. 5463-5479 ◽

Cited By ~ 4

Author(s):

Soumya Sundar Kumar ◽

Krishnendu Manna ◽

Asima Das

Keyword(s):

Heat Stress ◽

Coconut Water ◽

Testicular Damage ◽

Tender Coconut Water ◽

Tender Coconut

Tender Coconut Water (TCW) prevents heat stress (HS)-induced testicular damage.

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High-Throughput Phenotyping Approach for the Evaluation of Heat Stress in Korean Ginseng (Panax ginseng Meyer) Using a Hyperspectral Reflectance Image

Sensors ◽

10.3390/s21165634 ◽

2021 ◽

Vol 21 (16) ◽

pp. 5634

Author(s):

Eunsoo Park ◽

Yun-Soo Kim ◽

Mohammad Kamran Omari ◽

Hyun-Kwon Suh ◽

Mohammad Akbar Faqeerzada ◽

...

Keyword(s):

Heat Stress ◽

Discriminant Analysis ◽

Panax Ginseng ◽

Near Infrared ◽

Short Wave ◽

Hyperspectral Images ◽

Projection Algorithm ◽

Validation Data ◽

Linear Discriminant ◽

Successive Projection

Panax ginseng has been used as a traditional medicine to strengthen human health for centuries. Over the last decade, significant agronomical progress has been made in the development of elite ginseng cultivars, increasing their production and quality. However, as one of the significant environmental factors, heat stress remains a challenge and poses a significant threat to ginseng plants’ growth and sustainable production. This study was conducted to investigate the phenotype of ginseng leaves under heat stress using hyperspectral imaging (HSI). A visible/near-infrared (Vis/NIR) and short-wave infrared (SWIR) HSI system were used to acquire hyperspectral images for normal and heat stress-exposed plants, showing their susceptibility (Chunpoong) and resistibility (Sunmyoung and Sunil). The acquired hyperspectral images were analyzed using the partial least squares-discriminant analysis (PLS-DA) technique, combining the variable importance in projection and successive projection algorithm methods. The correlation of each group was verified using linear discriminant analysis. The developed models showed 12 bands over 79.2% accuracy in Vis/NIR and 18 bands with over 98.9% accuracy at SWIR in validation data. The constructed beta-coefficient allowed the observation of the key wavebands and peaks linked to the chlorophyll, nitrogen, fatty acid, sugar and protein content regions, which differentiated normal and stressed plants. This result shows that the HSI with the PLS-DA technique significantly differentiated between the heat-stressed susceptibility and resistibility of ginseng plants with high accuracy.

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