A lettuce moisture detection method based on terahertz time-domain spectroscopy

ABSTRACT: For non-destructive detection of water stress in lettuce, terahertz time-domain spectroscopy (THz-TDS) was used to quantitatively analyze water content in lettuce. Four gradient lettuce water contents were used . Spectral data of lettuce were collected by a THz-TDS system, and denoised using the S-G derivative, Savitzky-Golay (S-G) smoothing and normalization filtering. The fitting effect of the pretreatment method was better than that of regression fitting, and the S-G derivative fitting effect was obtained. Then a calibration set and a verification set were divided by the Kennan-Stone algorithm, sample set partitioning based on joint X-Y distance (SPXY) algorithm, and the random sampling (RS) algorithm, and the parameters of RS were optimized by regression fitting. The stability competitive adaptive reweighted sampling, iteratively retained information variables and interval combination optimization were used to select characteristic wavelengths, and then continuous projection was used on basis of the three algorithms above. After the successive projection algorithm was re-screened, partial least squares regression was used into modeling. The regression coefficients Rc 2 and RMSEC reach 0.8962 and 412.5% respectively, and Rp 2 and RMSEP of the verification set are 0.8757 and 528.9% respectively.

Blueberry bud freeze damage detection using optical sensors: Identification of spectral features through hyperspectral imagery

BACKGROUND: Highbush blueberry (Vaccinium corymbosum), the species primarily grown in the state of Washington, U.S., is relatively cold hardy. However, low temperatures in winter and early spring can still cause freeze damage to the buds. OBJECTIVE: This study intended to explore hyperspectral imaging (HSI) for detecting freeze induced bud damage. Blueberry buds (c.v. Duke) were collected over two seasons and tested in the laboratory to detect damage at four typical phenological stages. METHODS: The HSI data was acquired via line scan HSI system with spectral wavelength ranging from 517 to 1729 nm for buds grouped into either normal or injured mortalities. The successive projection algorithm was employed for pertinent feature wavelength selection. Analysis of variance and linear regression were then applied for evaluating sensitivity of feature wavelengths. RESULTS: Overall, five salient wavelengths (706, 723, 872, 1384, and 1591 nm) were selected to detect bud freeze injury. A quadratic discriminant analysis method-based analysis verified reliability of these five wavelengths in bud damage detection with overall accuracy in the ranges of 64 to 82%for the test datasets of each stage in two seasons. CONCLUSIONS: This study indicated potential of optical sensing to identify the injured buds using five salient wavelengths.

High-Throughput Phenotyping Approach for the Evaluation of Heat Stress in Korean Ginseng (Panax ginseng Meyer) Using a Hyperspectral Reflectance Image

Panax ginseng has been used as a traditional medicine to strengthen human health for centuries. Over the last decade, significant agronomical progress has been made in the development of elite ginseng cultivars, increasing their production and quality. However, as one of the significant environmental factors, heat stress remains a challenge and poses a significant threat to ginseng plants’ growth and sustainable production. This study was conducted to investigate the phenotype of ginseng leaves under heat stress using hyperspectral imaging (HSI). A visible/near-infrared (Vis/NIR) and short-wave infrared (SWIR) HSI system were used to acquire hyperspectral images for normal and heat stress-exposed plants, showing their susceptibility (Chunpoong) and resistibility (Sunmyoung and Sunil). The acquired hyperspectral images were analyzed using the partial least squares-discriminant analysis (PLS-DA) technique, combining the variable importance in projection and successive projection algorithm methods. The correlation of each group was verified using linear discriminant analysis. The developed models showed 12 bands over 79.2% accuracy in Vis/NIR and 18 bands with over 98.9% accuracy at SWIR in validation data. The constructed beta-coefficient allowed the observation of the key wavebands and peaks linked to the chlorophyll, nitrogen, fatty acid, sugar and protein content regions, which differentiated normal and stressed plants. This result shows that the HSI with the PLS-DA technique significantly differentiated between the heat-stressed susceptibility and resistibility of ginseng plants with high accuracy.

The Application of UAV-Based Hyperspectral Imaging to Estimate Crop Traits in Maize Inbred Lines

Crop traits such as aboveground biomass (AGB), total leaf area (TLA), leaf chlorophyll content (LCC), and thousand kernel weight (TWK) are important indices in maize breeding. How to extract multiple crop traits at the same time is helpful to improve the efficiency of breeding. Compared with digital and multispectral images, the advantages of high spatial and spectral resolution of hyperspectral images derived from unmanned aerial vehicle (UAV) are expected to accurately estimate the similar traits among breeding materials. This study is aimed at exploring the feasibility of estimating AGB, TLA, SPAD value, and TWK using UAV hyperspectral images and at determining the optimal models for facilitating the process of selecting advanced varieties. The successive projection algorithm (SPA) and competitive adaptive reweighted sampling (CARS) were used to screen sensitive bands for the maize traits. Partial least squares (PLS) and random forest (RF) algorithms were used to estimate the maize traits. The results can be summarized as follows: The sensitive bands for various traits were mainly concentrated in the near-red and red-edge regions. The sensitive bands screened by CARS were more abundant than those screened by SPA. For AGB, TLA, and SPAD value, the optimal combination was the CARS-PLS method. Regarding the TWK, the optimal combination was the CARS-RF method. Compared with the model built by RF, the model built by PLS was more stable. This study provides guiding significance and practical value for main trait estimation of maize inbred lines by UAV hyperspectral images at the plot level.

Projection Methods for Uniformly Convex Expandable Sets

Many problems in medical image reconstruction and machine learning can be formulated as nonconvex set theoretic feasibility problems. Among efficient methods that can be put to work in practice, successive projection algorithms have received a lot of attention in the case of convex constraint sets. In the present work, we provide a theoretical study of a general projection method in the case where the constraint sets are nonconvex and satisfy some other structural properties. We apply our algorithm to image recovery in magnetic resonance imaging (MRI) and to a signal denoising in the spirit of Cadzow’s method.

Hyperspectral Wavelength Selection and Integration for Bruise Detection of Korla Pears

Wavelength selection is a challenging job for the detection of the bruises on pears using hyperspectral imaging. Most modern research used the feature wavelength set selected by a single selection method which is generally unable to handle the wide variability of the hyperspectral data. A novel framework was proposed in this work to increase the performance of the bruise detection, through combining three state-of-the-art variable selection methods and the concept of feature-level integration. Successive projection algorithm, competitive adaptive reweighted sampling, and RELIEF were first applied to the spectra of the Korla pear, respectively. Then, the corresponding feature wavelength subsets were integrated and an optimal feature wavelength set was constructed. An ELM-based classifier was employed for the pear bruise identification finally. Experimental results demonstrated that the feature wavelength integration resulted in lower detection errors. The proposed method is simple and promising for bruise detection of Korla pears, and it can be utilized for other types of defects on fruits.

Detection of Moldy Cores in Apples with Near-Infrared Transmission Spectroscopy Based on Wavelet and BP Network

Moldy cores in apples are not initially obvious from the outside of the fruit, so developing methods to detect moldy cores is an important area of research in the apple industry. The objective of this study was to improve the ability of near-infrared spectrometry to detect moldy cores in apples. Transmission spectra were recorded for 200 apple samples in the range of 200–1100[Formula: see text]nm, and 140 and 60 samples were randomly selected as training and test sets, respectively. Signal de-noising was performed by wavelet thresholding based on the results of orthogonal experiments. The best wavelengths for discriminating between healthy and diseased apples were selected by a successive projection algorithm (SPA). The extracted wavelengths were used as the input in a back propagation artificial neural network (BP-ANN). Through these experiments, this study compared the correct recognition rates using different ratios of training to test numbers in the model, and functions in the hidden and output layers of the BP-ANN. The proposed method achieved the highest accuracies of 95.00% and 95.71% for the test and training sets, respectively. This method could be used to develop a portable instrument for detecting moldy cores in apples.