scholarly journals Antibody titers against the Alpha, Beta, Gamma, and Delta variants of SARS-CoV-2 induced by BNT162b2 vaccination measured using automated chemiluminescent enzyme immunoassay

2021 ◽  
Author(s):  
Hideaki Kato ◽  
Kei Miyakawa ◽  
Norihisa Ohtake ◽  
Hirofumi Go ◽  
Yutaro Yamaoka ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Antibody Titers ◽  
Alpha Beta ◽  
Chemiluminescent Enzyme Immunoassay

scholarly journals Antibody titers against the Alpha, Beta, Gamma, and Delta variants of SARS-CoV-2 induced by BNT162b2 vaccination measured using automated chemiluminescent enzyme immunoassay

2021 ◽  
Author(s):  
Hideaki Kato ◽  
Kei Miyakawa ◽  
Norihisa Ohtake ◽  
Hirofumi Go ◽  
Yutaro Yamaoka ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Herd Immunity ◽  
Original Strain ◽  
Older Individuals ◽  
Factors Affecting ◽  
Positive Correlation ◽  
Igg Index ◽  
Antibody Titers ◽  
Alpha Beta ◽  
Chemiluminescent Enzyme Immunoassay

Background Levels of 50% neutralizing titer (NT50) reflect the vaccine-induced humoral immunity after the vaccination against the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Measurements of NT50 are difficult to implement in large quantities. A high-throughput laboratory test is expected for determining the level of herd immunity against SARS-CoV-2. Methods We analyzed samples from 168 Japanese healthcare workers who had completed two doses of the BNT162b2 vaccine. We analyzed immunoglobulin G (IgG) index values against spike protein (SP) using automated chemiluminescent enzyme immunoassay system AIA-CL and analyzed the background factors affecting antibody titer. SP IgG index was compared with 50% neutralization titers. Results The median SP IgG index values of the subjects (mean age = 43 years; 75% female) were 0.1, 1.35, 60.80, and 97.35 before and at 2, 4, and 6 weeks after the first dose, respectively. At 4 and 6 weeks after the first dose, SP IgG titers were found to have positive correlation with NT50 titer (r=0.7535 in 4 weeks; r=0.4376 in 6 weeks). Proportions of the SP IgG index values against the Alpha, Beta, Gamma, and Delta variants compared with the original strain were 2.029, 0.544, 1.017, and 0.6096 respectively. Older age was associated with lower SP IgG titer index 6 weeks after the first dose. Conclusions SP IgG index values were raised at 3 weeks after two doses of BNT162b2 vaccination and have positive correlation with NT50. SP IgG index values were lower in the older individuals and against Beta and Delta strain.

scholarly journals Immunochromatography and chemiluminescent enzyme immunoassay for COVID-19 diagnosis

2021 ◽  
Vol 27 (6) ◽  
pp. 915-918 ◽  
Author(s):  
Toshiaki Ishii ◽  
Masakazu Sasaki ◽  
Kageto Yamada ◽  
Daiki Kato ◽  
Hiroyoshi Osuka ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Chemiluminescent Enzyme Immunoassay

Development and Validation of an Ultrasensitive Chemiluminescent Enzyme Immunoassay for Aflatoxin M1in Milk

2005 ◽  
Vol 53 (9) ◽  
pp. 3300-3305 ◽  
Author(s):  
Maria Magliulo ◽  
Mara Mirasoli ◽  
Patrizia Simoni ◽  
Rosella Lelli ◽  
Ottavio Portanti ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Development And Validation ◽  
Chemiluminescent Enzyme Immunoassay

Evaluation of cardiac assays on a benchtop chemiluminescent enzyme immunoassay analyzer, PATHFAST

2008 ◽  
Vol 375 (1) ◽  
pp. 144-146 ◽  
Author(s):  
Takashi Kurihara ◽  
Atsushi Yanagida ◽  
Hiroyuki Yokoi ◽  
Atsushi Koyata ◽  
Takeshi Matsuya ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Chemiluminescent Enzyme Immunoassay

scholarly journals CORRELATION OF MONOCYTE COUNT, MLR AND NLCR WITH PRESEPSIN LEVEL IN SIRS (Hubungan Jumlah Monosit, MLR dan NLCR dengan Kadar Presepsin pada SIRS)

2018 ◽  
Vol 22 (3) ◽  
pp. 212
Author(s):  
Nurmalia PS ◽  
N. Suci W ◽  
Imam BW
Keyword(s):  
Systemic Inflammatory Response Syndrome ◽  
Inflammatory Response ◽  
Enzyme Immunoassay ◽  
Lymphocyte Count ◽  
Systemic Inflammatory Response ◽  
Monocyte Count ◽  
Lymphocyte Ratio ◽  
Count Ratio ◽  
Hematology Analyzer ◽  
Chemiluminescent Enzyme Immunoassay

Systemic Inflammatory Response Syndrome (SIRS) mempunyai kebahayaan tinggi terjadi sepsis dan kematian. Nilai jumlahkeseluruhan leukosit merupakan salah satu peramal pasien SIRS dengan bakteriemia. Pemeriksaan jumlah monosit, angka bandinglimfosit Monocyte-Lymphocyte Ratio (MLR), Neutrophil-Lymphocyte Count Ratio (NLCR) dapat diketahui dengan pemeriksaan leukosit.Presepsin telah diteliti untuk mencerminkan kondisi sepsis. Penelitian ini bertujuan untuk mengetahui keberadaan hubungan jumlahmonosit, MLR dan NLCR dengan presepsin di SIRS lewat pembuktian. Ada 34 pasien SIRS di ICU RSUP Dr. Kariadi, diambil secaraberturutan antara selama bulan Januari−Februari 2014. Pemeriksaan darah rutin dengan hematology analyzer. MLR dan NLCR dihitung secara manual. Kadar presepsin ditentukan dengan metode Chemiluminescent Enzyme Immunoassay (CLEIA). Uji kenasabanPearson untuk hubungan MLR dan NLCR dengan presepsin. Uji kenasaban Spearman untuk jumlah monosit dengan presepsin. Kadarpresepsin subjek penelitian 286–15687 pg/mL. Terdapat 23(67,8%) subjek yang mempunyai jumlah monosit dalam rentang nilai rujukan.24(70,6%) dan memiliki jumlah neutrofil absolut lebih besar dari rentang nilai rujukan, sedangkan 21(61,8%) mempunyai jumlahlimfosit absolut dalam rentang nilai rujukan. Hubungan jumlah monosit dengan presepsin mempunyai nilai r= -0,204; p=0,247;yang terkait MLR dengan presepsin r=0,163; p=0,358; sedangkan NLCR dengan presepsin r=0,345; p=0,046. Didasari telitian ini,dapat disimpulkan tidak terdapat hubungan bermakna antara jumlah monosit dan MLR dengan presepsin, selain itu didapatkan pulahubungan positif berarti antara NLCR dan presepsin di SIRS.

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