Assessing gene expression during pathogenesis: Use of qRT-PCR to follow toxin production in the entomopathogenic fungus Beauveria bassiana during infection and immune response of the insect host Triatoma infestans

2015 ◽  
Vol 128 ◽  
pp. 14-21 ◽  
Author(s):  
Luciana S. Lobo ◽  
Christian Luz ◽  
Éverton K.K. Fernandes ◽  
M. Patricia Juárez ◽  
Nicolás Pedrini
Keyword(s):  
Gene Expression ◽  
Immune Response ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungus ◽  
Toxin Production ◽  
Triatoma Infestans ◽  
Insect Host ◽  
Qrt Pcr ◽  
Fungus Beauveria Bassiana

scholarly journals Generation of Stilbene Glycoside with Promising Cell Rejuvenation Activity through Biotransformation by the Entomopathogenic Fungus Beauveria bassiana

Biomedicines ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 555
Author(s):  
Sang Keun Ha ◽  
Min Cheol Kang ◽  
Seulah Lee ◽  
Om Darlami ◽  
Dongyun Shin ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungus ◽  
Spectroscopic Data ◽  
Treated Group ◽  
Qrt Pcr ◽  
Skin Keratinocytes ◽  
Tnf Α ◽  
Sugar Unit ◽  
Ifn Γ ◽  
Fungus Beauveria Bassiana

A stilbene glycoside (resvebassianol A) (1) with a unique sugar unit, 4-O-methyl-D-glucopyranose, was identified through biotransformation of resveratrol (RSV) by the entomopathogenic fungus Beauveria bassiana to obtain a superior RSV metabolite with enhanced safety. Its structure, including its absolute configurations, was determined using spectroscopic data, HRESIMS, and chemical reactions. Microarray analysis showed that the expression levels of filaggrin, HAS2-AS1, and CERS3 were higher, while those of IL23A, IL1A, and CXCL8 were lower in the resvebassianol A-treated group than in the RSV-treated group, as confirmed by qRT-PCR. Compound 1 exhibited the same regenerative and anti-inflammatory effects as RSV with no cytotoxicity in skin keratinocytes and TNF-α/IFN-γ-stimulated HIEC-6 cells, suggesting that compound 1 is a safe and stable methylglycosylated RSV. Our findings suggest that our biotransformation method can be an efficient biosynthetic platform for producing a broad range of natural glycosides with enhanced safety.

Endophytic relationship between an entomopathogenic fungus Beauveria bassiana and corn (zea mays)

1995 ◽  
Vol 53 ◽  
pp. 988-989
Author(s):  
Bruce L. Wagner ◽  
Leslie C. Lewis
Keyword(s):  
Zea Mays ◽  
Beauveria Bassiana ◽  
Ostrinia Nubilalis ◽  
Entomopathogenic Fungus ◽  
Short Distance ◽  
Germ Tube ◽  
Leaf Surface ◽  
Insect Host ◽  
Lepidoptera Pyralidae ◽  
Fungus Beauveria Bassiana

Fungi are known to colonize plants, usually as phytopathogens or symbionts. This is the first report of the entomopathogenic fungus, Beauveria bassiana (Balsamo) Vuillemin (Deuteromycotina: Hyphomycetes) penetrating and colonizing a plant in a manner similar to when it invades a typical insect host Ostrinia nubilalis Hübner (Lepidoptera: Pyralidae). Electron microscopy was used to document this fungus penetrating the leaves of corn (Zea mays) and establishing an endophytic relationship within the plant.Several studies have shown B. bassiana to adhere to insect cuticle and penetrate with or without forming appressorial penetration structures. Investigations have concluded that the young, germinating hyphae may produce sequentially a proteinase followed by chitinase to dissolve the cuticular envelope of target insects. Within three days after inoculation, viable B. bassiana conidia germinated and formed vegetative mycelia which grew randomly over the corn leaf surface (Fig. 1). Often a germ tube is formed from a conidium and elongates only a short distance before terminating its growth and penetrating the leaf surface (Figs. 2 and 3).

scholarly journals Management of the Egyptian alfalfa weevil, Hypera brunneipennis (Boheman) (Coleoptera: Curculionidae), in the alfalfa, Medicago sativa L., using the entomopathogenic fungus, Beauveria bassiana (Balsamo) Vuillemin

2019 ◽  
Vol 29 (1) ◽  
Author(s):  
Monir M. M. El Husseini
Keyword(s):  
Medicago Sativa ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungus ◽  
Larval Population ◽  
Alfalfa Weevil ◽  
Alfalfa Field ◽  
Medicago Sativa L ◽  
Hypera Brunneipennis ◽  
Fungus Beauveria Bassiana ◽  
Sweep Net

AbstractLarval and adult populations of the Egyptian alfalfa weevil (EAW) Hypera brunneipennis (Boheman) (Coleoptera: Curculionidae) was monitored after application of the entomopathogenic fungus Beauveria bassiana in the alfalfa field (Medicago sativa L.) in two successive seasons 2014/2015 and 2015/2016. The second and last generation of the weevil on April 10, 2016, was controlled by only one application with the conidiospores of the entomopathogenic fungus B. bassiana (3 × 108 spores/ml). Accordingly, the larval population decreased from 16.07 ± 1.09 in season 2015/2016 to 7.37 ± 0.05 individuals/50 sweep net double strokes in season 2016/2017. Also, the adult weevil’s population decreased from 5.66 ± 0.8 to 2.55 ± 0.6 individuals/50 sweep net double strokes in the two seasons, respectively. 39.66% mortality rate was recorded in the Hypera brunneipennis adults aestivated under loose bark of the surrounding eucalyptus trees, which received the application of B. bassiana in the field. Another application with the fungus, targeting the second generation of the pest adults in alfalfa each season, will undoubtedly lead to a further decrease in the pest population.

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