Molecular Detection of common Carbapenemase Resistance Genes in nosocomial pathogens isolated from Neonatal ICU at a major tertiary care hospital in Mecca

Integrons play an important role in the dissemination of resistance genes among bacteria. Nearly 70% of highly resistant gram-negative bacteria isolated at a tertiary care hospital harbored an integron. Epidemiologic analysis suggests that horizontal gene transfer is an important mechanism of resistance spread and has a greater contribution than cross-transmission to levels of resistance in settings where highly resistant gram-negative bacteria are endemic.

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Antimicrobial use and antimicrobial resistance in nosocomial pathogens at a tertiary care hospital in Pune

Medical Journal Armed Forces India ◽

10.1016/j.mjafi.2014.12.024 ◽

2015 ◽

Vol 71 (2) ◽

pp. 112-119 ◽

Cited By ~ 3

Author(s):

Velu Nair ◽

Dinesh Sharma ◽

A.K. Sahni ◽

Naveen Grover ◽

S. Shankar ◽

...

Keyword(s):

Antimicrobial Resistance ◽

Tertiary Care Hospital ◽

Tertiary Care ◽

Antimicrobial Use ◽

Care Hospital ◽

Nosocomial Pathogens

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Death audit in the neonatal ICU of a tertiary care hospital in Bangladesh: a retrospective chart review

The Journal of Maternal-Fetal & Neonatal Medicine ◽

10.1080/14767058.2017.1391779 ◽

2017 ◽

Vol 32 (5) ◽

pp. 776-780 ◽

Cited By ~ 1

Author(s):

Sanjoy Kumer Dey ◽

Sharmin Afroze ◽

Tariqul Islam ◽

Ismat Jahan ◽

Mohammad Kamrul Hassan Shabuj ◽

...

Keyword(s):

Chart Review ◽

Tertiary Care Hospital ◽

Tertiary Care ◽

Retrospective Chart Review ◽

Care Hospital ◽

Neonatal Icu

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Frequency of MexA gene in pseudomonas aeruginosa isolated from clinical samples of a Tertiary Care Hospital in Pakistan.

The Professional Medical Journal ◽

10.29309/tpmj/2020.27.11.4589 ◽

2020 ◽

Vol 27 (11) ◽

pp. 2389-2393

Author(s):

Syed Luqman Shuaib ◽

Amina Gul ◽

Jawad Ahmed ◽

Noor Rehman ◽

Liaqt Ali ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Resistance Genes ◽

Efflux Pump ◽

Tertiary Care Hospital ◽

Tertiary Care ◽

Clinical Samples ◽

Specific Gene ◽

Care Hospital ◽

Conventional Pcr ◽

Isolation And Identification

The drug resistance genes are responsible to preserve the Pseudomonas. This also happens in the case of Mex drug efflux pumps and expression increase of MexA, MexB and OprM genes in Pseudomonas aeruginosa, when grown in sub-inhibitory concentrations of antibiotics. Objectives: This study was designed to detect the MexA gene of Pseudomonas aeruginosa resistant strains in tertiary care hospital, Peshawar. Study Design: Cross-sectional study. Setting: Department of Pathology, Khyber Teaching Hospital, Peshawar, Pakistan. Period: 14 months duration from April 2015 to May 2016. Material & Methods: The specimens including burn wound swabs, pus and urine were obtained from different patients and were processed on blood agar and MacConkey medium for isolation and identification. Conventional PCR was performed for the MexA gene on 50 specimens. Results: The simple conventional PCR was done for MexA (The Mex AB OprM operon resistance genes) and the O-antigen acetylase gene (the species-specific gene) separately, gave positive bands for 49 out of the 50 specimens. Our finding confirms the presence of the MexA gene (and hence most probably MexABOprM operon) in 49 out of 50 specimens of Pseudomonas aeruginosa. Conclusion: Among other resistance mechanisms to antibiotics and disinfectants, the MexABOprM efflux pump might have a role.

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