Molecular detection of seven pathogens causing urethritis / vaginosis in a tertiary care hospital, Bangkok, Thailand

2016 ◽  
Author(s):  
Narong Chaihongsa
Keyword(s):  
Molecular Detection ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Care Hospital

scholarly journals Molecular Detection of blaoxa-23 Gene from Carbapenem Resistant Acinetobacter Baumannii Isolated from a Tertiary Care Hospital of Nepal

2020 ◽  
Author(s):  
Laxmi Neupane ◽  
Anil Kumar Shah ◽  
Binod Rayamajhee ◽  
Anjana Singh
Keyword(s):  
Acinetobacter Baumannii ◽  
Pathogenic Bacteria ◽  
Molecular Detection ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
High Rate ◽  
Antibiotics Resistance ◽  
Care Hospital ◽  
Carbapenem Resistant ◽  
Carbapenemase Gene

Abstract Background: Infections associated with Acinetobacter baumannii are increasing in many parts of the world especially the healthcare associated infections (HAIs). Antibiotics resistance is a great concern of public health which is either an inherent or adaptation property of microorganisms to resist the action of antibiotics. Carbapenem resistance, mainly among gram-negative bacteria is an ongoing problem that causes serious infections and dramatically limits the treatment alternatives. The prospective cross-sectional study was designed to detect the blaOXA-23 gene from carbapenem-resistant A. baumannii isolates in a tertiary care hospital of Nepal. Methods: A total of 380 clinical specimens (tracheal aspirate, urine, sputum, blood and wound samples, Foleys tips, and catheter tips) were collected from the study population and were examined by microbiological procedures including Gram’s staining, culture, and various biochemical tests. Antibiotic susceptibility testing (AST) was done as per the protocol of Kirby-Bauer disk diffusion technique and the CLSI guidelines while screening of carbapenemase production was checked through Modified Hodge Test (MHT) using Meropenem (10µg) disc. All the phenotypically positive results for carbapenemase production were further analysed by PCR and agarose gel electrophoresis for molecular detection of the blaOXA-23 gene. Results: Among 380 specimens analysed, 55.3% (210/310) of samples were positive for bacterial growth where 15.7% (33/210) of bacterial isolates were A. baumannii and 69.7% (23/33) were carbapenem-resistant. High prevalence (21.2%) of A. baumannii was among the patients of age group 51-60 followed by 41-50 years (18.2%) years but the result was statistically insignificant (P>0.05) and more isolates were from ICU (60.6%) followed by post-operative patients (18.2%) The blaOXA-23 carbapenemase gene was found in 82.6% (19/23) of meropenem resistant isolates while 97% isolates of A. baumannii were susceptible to colistin. Conclusion: The high rate of antibiotic resistance is funnelling the therapeutic options for the treatment of infections associated with A. baumannii which clearly shows a need for rational use of antibiotics. Systematic network surveillance should be established for monitoring and controlling the spread of the antibiotic-resistant gene of pathogenic bacteria especially in a resource-limited clinical setting like Nepal.

Confidence toward vaccination as reported by parents of children admitted to a tertiary care hospital in Riyadh, Saudi Arabia: A cross sectional study

Vacunas ◽  
2020 ◽  
Vol 21 (2) ◽  
pp. 95-104 ◽  
Author(s):  
Y.M. AlGoraini ◽  
N.N. AlDujayn ◽  
M.A. AlRasheed ◽  
Y.E. Bashawri ◽  
S.S. Alsubaie ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Cross Sectional Study ◽  
Care Hospital ◽  
Sectional Study ◽  
Cross Sectional

Abstract #1180: Trimester Specific Reference Interval for Thyroid Hormones During Pregnancy at a Tertiary Care Hospital in Haryana, India.

2016 ◽  
Vol 22 ◽  
pp. 293
Author(s):  
Rajesh Rajput ◽  
Bhagat Singh ◽  
Vasudha Goel ◽  
Amit Verma ◽  
Shashi Seth ◽  
...  
Keyword(s):  
Thyroid Hormones ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Reference Interval ◽  
Specific Reference ◽  
Care Hospital

TO DETERMINE THE ROLE OF CA125 IN THE DIAGNOSIS OF ACUTE APPENDICITIS

2020 ◽  
Vol 4 (7) ◽  
Author(s):  
Vinod Kumar ◽  
Bhupen Songra ◽  
Richa Jain ◽  
Deeksha Mehta
Keyword(s):  
Acute Appendicitis ◽  
Clinical Diagnosis ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Ca 125 ◽  
Care Hospital ◽  
Serum Samples ◽  
Roc Curve Analysis ◽  
Surgery Department

Background: the present study was under taken to determine the role of CA-125 in the diagnosis of acute appendicitis (AA), to prevent its complications and also in preventing negative appendicectomies in tertiary care hospital. Methods: The study was conducted at a tertiary care and research center between 01/03/2018 to 30/06/2019. Patients admitted to the surgery department with diagnosis of AA were considered for the study. After informed consent, a, standardized history was obtained as a case Performa. Serum samples from all the cases with clinical diagnosis of AA were obtained and stored. Only the cases with histopathologically approved AA were included in the study. Cases operated for clinical diagnosis of AA, but not histopathologically proven AA was not included in the study. CA125 levels in cases with definitive diagnosis of AA were measured. Results: In present study, ROC curve analysis revealed the sensitivity of 87.27 % and specificity of 90.91 % when the CA 125 cut-off value of > 16.8 was taken to diagnose acute appendicitis. AUC was 0.911 with a standard error of 0.0292. Conclusion: In this study we have observed that CA125 showed a positive correlation with acute appendicitis, that was statistically not significant (P>0.05). We didn’t evaluate the correlation with the disease severity. We consider that CA125 can be used as a marker in acute appendicitis cases although further research is still needed. Keywords: CA125, Acute Appendicitis, Surgery.

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