Pretreatment of microalgal biomass to improve the enzymatic hydrolysis of carbohydrates by ultrasonication: Yield vs energy consumption

The results of the studies indicate an increase in the efficiency of enzymatic hydrolysis of wheat grain subjected to infrared irradiation in the production of feed molasses. It was found that the duration of enzymatic hydrolysis of grain to significant concentrations of sugars in feed molasses is reduced by 2 times and energy costs are reduced by 40 %, and the concentration of sugars increases at the end of the process by 4.0 % compared to existing technologies.

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Effects of milling combined with extremely low-liquid ammonia (ELLA) pretreatment to enhance enzymatic hydrolysis of corn stover

Thu Dau Mot University Journal of Science ◽

10.37550/tdmu.ejs/2021.01.153 ◽

2021 ◽

pp. 105-114

Keyword(s):

Energy Consumption ◽

Enzymatic Hydrolysis ◽

Ball Milling ◽

Large Scale ◽

Liquid Ammonia ◽

Milling Time ◽

Lignocellulosic Feedstock ◽

Pretreatment Time ◽

Hydrolysis Of ◽

Ball Milling Time

Lignocellulosic biomass is one of the largest carbohydrate sources and has huge potential for biofuels production. However, the problem with lignocellulosic feedstock is that it has useful sugars locked in by lignin, hemicellulose, and cellulose. Some kind of pretreatment; therefore is needed to make carbohydrate accessible which later can be fermented to produce ethanol. The results from this research indicated that the yields of glucan (93%) and xylan (82.8%) were improved by using milling combined with ELLA pretreatment. The optimal enzymatic hydrolysis efficiencies were obtained under 10 min for ball milling time, pretreatment at 1 h, temperature at 150°C, S/L = 0.5 and ammonia loading at 0.25 g-NH3/g-biomass. This method reduced the pretreatment time and short milling time and thus has potential of reducing the energy consumption and promising the application in the large scale.

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Enzymatic Hydrolysis of Residual Carbohydrates from Microalgal Biomass Chlorella vulgaris

Revista Virtual de Química ◽

10.21577/1984-6835.20210106 ◽

2021 ◽

Author(s):

Jorge Luis Sánchez Ortega ◽

Jorge Enrique Lopez Galán

Keyword(s):

Enzymatic Hydrolysis ◽

Chlorella Vulgaris ◽

Microalgal Biomass ◽

Hydrolysis Of

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From biorefineries to bioproducts: conversion of pretreated pulp from biorefining streams to lignocellulose nanofibers

TAPPI Journal ◽

10.32964/tj18.4.233 ◽

2019 ◽

Vol 18 (4) ◽

pp. 233-241

Author(s):

CHENGGUI SUN ◽

RICHARD CHANDRA ◽

YAMAN BOLUK

Keyword(s):

Energy Consumption ◽

Enzymatic Hydrolysis ◽

Rheological Behavior ◽

Material Basis ◽

Softwood Pulp ◽

Energy Consumptions ◽

Hardwood Pulp ◽

The Stability ◽

Lignocellulose Nanofibers

This study investigates the use of pretreatment and enzymatic hydrolysis side streams and conversion to lignocellulose nanofibers. We used a steam-exploded and partial enzymatic hydrolyzed hardwood pulp and an organosolv pretreated softwood pulp to prepare lignocellulose nanofibers (LCNF) via microfluidization. The energies applied on fibrillation were estimated to examine the energy consumption levels of LCNF production. The energy consumptions of the fibrillation processes of the hardwood LCNF production and the softwood LCNF production were about 7040-14080 kWh/ton and 4640 kWh/ton on a dry material basis, respectively. The morphology and dimension of developed hardwood and softwood LCNFs and the stability and rheological behavior of their suspensions were investigated and are discussed.

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Microwave-assisted enzymatic hydrolysis of starch

10.3390/ecsoc-13-00159 ◽

2009 ◽

Cited By ~ 1

Author(s):

Marcin Lukasiewicz ◽

Anna Osowiec ◽

Magdalena Marciniak

Keyword(s):

Enzymatic Hydrolysis ◽

Microwave Assisted ◽

Hydrolysis Of

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Analyses of enzymatic hydrolysis of bagasse cane of sugar for obtention of ethanol employing a cocktail of natives commercials enzymes.

10.3390/mol2net-04-05300 ◽

2018 ◽

Author(s):

Ángel Batallas ◽

Erenio González ◽

Carmen Salvador ◽

Jonathan Villavicencio ◽

Humberto González Gavilánez ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Hydrolysis Of

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Faculty Opinions recommendation of Enzymatic hydrolysis of pneumococcal capsular polysaccharide renders the bacterium vulnerable to host defense.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.733387483.793547056 ◽

2018 ◽

Author(s):

Mattias Collin

Keyword(s):

Enzymatic Hydrolysis ◽

Host Defense ◽

Capsular Polysaccharide ◽

Hydrolysis Of

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Immobilized Nanoparticles-Mediated Enzymatic Hydrolysis of Cellulose for Clean Sugar Production: A Novel Approach

Current Nanoscience ◽

10.2174/1573413714666180611081759 ◽

2019 ◽

Vol 15 (3) ◽

pp. 296-303 ◽

Cited By ~ 5

Author(s):

Swapnil Gaikwad ◽

Avinash P. Ingle ◽

Silvio Silverio da Silva ◽

Mahendra Rai

Keyword(s):

Catalytic Activity ◽

Enzymatic Hydrolysis ◽

Immobilized Enzyme ◽

Free Enzyme ◽

Magnetic Nanomaterials ◽

Sugar Production ◽

Cellulase Enzyme ◽

Enzymatic Hydrolysis Of Cellulose ◽

Hydrolysis Of Cellulose ◽

Hydrolysis Of

Background: Enzymatic hydrolysis of cellulose is an expensive approach due to the high cost of an enzyme involved in the process. The goal of the current study was to apply magnetic nanomaterials as a support for immobilization of enzyme, which helps in the repeated use of immobilized enzyme for hydrolysis to make the process cost-effective. In addition, it will also provide stability to enzyme and increase its catalytic activity. Objective: The main aim of the present study is to immobilize cellulase enzyme on Magnetic Nanoparticles (MNPs) in order to enable the enzyme to be re-used for clean sugar production from cellulose. Methods: MNPs were synthesized using chemical precipitation methods and characterized by different techniques. Further, cellulase enzyme was immobilized on MNPs and efficacy of free and immobilized cellulase for hydrolysis of cellulose was evaluated. Results: Enzymatic hydrolysis of cellulose by immobilized enzyme showed enhanced catalytic activity after 48 hours compared to free enzyme. In first cycle of hydrolysis, immobilized enzyme hydrolyzed the cellulose and produced 19.5 ± 0.15 gm/L of glucose after 48 hours. On the contrary, free enzyme produced only 13.7 ± 0.25 gm/L of glucose in 48 hours. Immobilized enzyme maintained its stability and produced 6.15 ± 0.15 and 3.03 ± 0.25 gm/L of glucose in second and third cycle, respectively after 48 hours. Conclusion: This study will be very useful for sugar production because of enzyme binding efficiency and admirable reusability of immobilized enzyme, which leads to the significant increase in production of sugar from cellulosic materials.

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