Organelle-Targeted Delivery of Biological Macromolecules Using the Protein Transduction Domain: Potential Applications for Peptide Aptamer Delivery into the Nucleus

2008 ◽  
Vol 380 (5) ◽  
pp. 777-782 ◽  
Author(s):  
Tomoaki Yoshikawa ◽  
Toshiki Sugita ◽  
Yohei Mukai ◽  
Natsue Yamanada ◽  
Kazuya Nagano ◽  
...  
Keyword(s):  
Targeted Delivery ◽  
Protein Transduction ◽  
Biological Macromolecules ◽  
Protein Transduction Domain ◽  
Potential Applications ◽  
Peptide Aptamer

scholarly journals Creation of Novel Protein Transduction Domain (PTD) Mutants by a Phage Display-Based High-Throughput Screening System

2006 ◽  
Vol 29 (8) ◽  
pp. 1570-1574 ◽  
Author(s):  
Yohei Mukai ◽  
Toshiki Sugita ◽  
Tomoko Yamato ◽  
Natsue Yamanada ◽  
Hiroko Shibata ◽  
...  
Keyword(s):  
Phage Display ◽  
High Throughput ◽  
High Throughput Screening ◽  
Protein Transduction ◽  
Protein Transduction Domain ◽  
Screening System ◽  
Novel Protein

Gene delivery using a derivative of the protein transduction domain peptide, K-Antp

Biomaterials ◽  
2010 ◽  
Vol 31 (7) ◽  
pp. 1858-1864 ◽  
Author(s):  
Sang-Hyun Min ◽  
Dong Min Kim ◽  
Mi Na Kim ◽  
Jiang Ge ◽  
Dong Chul Lee ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Protein Transduction ◽  
Protein Transduction Domain ◽  
Domain Peptide

scholarly journals Protein Transduction Domain of HIV-1 Tat Protein Promotes Efficient Delivery of DNA into Mammalian Cells

2001 ◽  
Vol 276 (28) ◽  
pp. 26204-26210 ◽  
Author(s):  
Akiko Eguchi ◽  
Teruo Akuta ◽  
Hajime Okuyama ◽  
Takao Senda ◽  
Haruhiko Yokoi ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Protein Transduction ◽  
Protein Transduction Domain ◽  
Tat Protein ◽  
Efficient Delivery ◽  
Hiv 1

scholarly journals Electron microscopy snapshots of single particles from single cells

2018 ◽  
Vol 294 (5) ◽  
pp. 1602-1608 ◽  
Author(s):  
Xiunan Yi ◽  
Eric J. Verbeke ◽  
Yiran Chang ◽  
Daniel J. Dickinson ◽  
David W. Taylor
Keyword(s):  
Electron Microscopy ◽  
Single Particle ◽  
Protein Complexes ◽  
Signal To Noise Ratio ◽  
Single Cells ◽  
Particle Analysis ◽  
Biological Macromolecules ◽  
Single Particle Analysis ◽  
Whole Cells ◽  
Potential Applications

Cryo-electron microscopy (cryo-EM) has become an indispensable tool for structural studies of biological macromolecules. Two additional predominant methods are available for studying the architectures of multiprotein complexes: 1) single-particle analysis of purified samples and 2) tomography of whole cells or cell sections. The former can produce high-resolution structures but is limited to highly purified samples, whereas the latter can capture proteins in their native state but has a low signal-to-noise ratio and yields lower-resolution structures. Here, we present a simple, adaptable method combining microfluidic single-cell extraction with single-particle analysis by EM to characterize protein complexes from individual Caenorhabditis elegans embryos. Using this approach, we uncover 3D structures of ribosomes directly from single embryo extracts. Moreover, we investigated structural dynamics during development by counting the number of ribosomes per polysome in early and late embryos. This approach has significant potential applications for counting protein complexes and studying protein architectures from single cells in developmental, evolutionary, and disease contexts.

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