scholarly journals An Isothermal, Multiplex Amplification Assay for Detection and Genotyping of Human Papillomaviruses in Formalin-Fixed, Paraffin-Embedded Tissues

2020 ◽  
Vol 22 (3) ◽  
pp. 419-428 ◽  
Author(s):  
Yi-Wei Tang ◽  
Lorena Lozano ◽  
Xin Chen ◽  
Troy D. Querec ◽  
Nora Katabi ◽  
...  
Keyword(s):  
Human Papillomaviruses ◽  
Formalin Fixed Paraffin ◽  
Multiplex Amplification ◽  
Formalin Fixed Paraffin Embedded ◽  
Amplification Assay ◽  
Formalin Fixed

scholarly journals Immunohistochemical Detection of early and late Human Papillomaviruses (HPV) proteins in Retinoblastoma tumor

2020 ◽  
Author(s):  
Nara Diniz Soares Pessoa ◽  
Thatiana Correia Melo ◽  
Rodrigo Pinheiro Araldi ◽  
Rofrigo Franco Carvalho ◽  
Willy Becak ◽  
...  
Keyword(s):  
Hpv Infection ◽  
Human Papillomaviruses ◽  
Immunohistochemical Detection ◽  
Hpv Dna ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Retinoblastoma Tumor ◽  
Formalin Fixed

In this study, we evaluated the presence of early and late Human Papillomavirus (HPV) proteins in retinoblastoma Brazilian patients. For this, 8 formalin-fixed paraffin-embedded retinoblastoma tissue blocks were used. HPV DNA presence was determined by in situ hybridization (ISH). Immunohistochemistry were performed to verify HPV16/18 E6, E1^E4, and L1 proteins. HPV was detected in all retinoblastoma tumors and viral DNA was labeled in tumor cells, retinal layers and optical nerve structures. In addition, E1^E4, E6 and L1 proteins were detected in all samples in the same areas where HPV DNA was detected. Our data showed the presence and expression of early and late HPV proteins in retinoblastoma tumors from Brazilian children. However, further studies should be performed to clarify the role of HPV infection in retinoblastoma tumor.

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