Optimization and kinetics of glucose production via enzymatic hydrolysis of mixed peels

This study aimed to analyze the kinetics of enzymatic hydrolysis of cellulose. Cellulase enzyme was obtained fromAspergillusnigergrown on filter paper and the hydrolysis process was carried out in solid state. The kinetic reviewed here was the relationship between glucose production and cellulose mass reduction to optimize the parameters of Monod equation at various moisture levels.

Download Full-text

Effect of Particle Size on the Kinetics of Enzymatic Hydrolysis of Microcrystalline Cotton Cellulose: a Modeling and Simulation Study

Applied Biochemistry and Biotechnology ◽

10.1007/s12010-018-2856-6 ◽

2018 ◽

Vol 187 (3) ◽

pp. 800-816 ◽

Cited By ~ 1

Author(s):

Ashwin Gaikwad

Keyword(s):

Particle Size ◽

Enzymatic Hydrolysis ◽

Modeling And Simulation ◽

Simulation Study ◽

Cotton Cellulose ◽

Effect Of Particle Size ◽

Kinetics Of ◽

Hydrolysis Of

Download Full-text

Kinetics of enzymatic hydrolysis of rice straw by the pretreatment with a bio-based basic ionic liquid under ultrasound

Process Biochemistry ◽

10.1016/j.procbio.2015.01.013 ◽

2015 ◽

Vol 50 (4) ◽

pp. 623-629 ◽

Cited By ~ 15

Author(s):

Chun-Yao Yang ◽

Tony J. Fang

Keyword(s):

Ionic Liquid ◽

Enzymatic Hydrolysis ◽

Rice Straw ◽

Basic Ionic Liquid ◽

Kinetics Of ◽

Hydrolysis Of

Download Full-text

Kinetics of Enzymatic Hydrolysis of Southeast Sulawesi Sago Starch

Asian Food Science Journal ◽

10.9734/afsj/2020/v18i130215 ◽

2020 ◽

pp. 53-61

Author(s):

Ansharullah Ansharullah ◽

Muhammad Natsir

Keyword(s):

Enzymatic Hydrolysis ◽

Maximum Velocity ◽

Enzyme Concentration ◽

Hydrolysis Rate ◽

Sago Starch ◽

Optimum Conditions ◽

Kinetics Of ◽

Hydrolysis Of ◽

Substrate Concentrations ◽

Menten Constant

The aims of this study were to characterize the kinetics of enzymatic hydrolysis of sago starch, obtained from Southeast Sulawesi Indonesia. The enzyme used for hydrolysis was bacterial ∝-amylase (Termamyl 120L from Bacillus licheniformis, E. C. 3.2.1.1). The method to determine the initial velocity (Vo) of the hydrolysis was developed by differentiation a nonlinear equation (NLE). The Vo of the hydrolysis was measured at various pH (6.0, 6.5,and 7.0), temperatures (40, 60, 75 and 95oC), enzyme concentrations (0.5, 1.0, 1.5 and 2.0 µg per mL) and in the presence of 70 ppm Ca++. The optimum conditions of this experiment were found to be at pH 6.5 – 7.0 and 75oC, and the Vo increased with increasing enzyme concentration. The Vo values at various substrate concentrations were also determined, which were then used to calculate the enzymes kinetics constant of the hydrolysis, including Michaelis-Menten constant (Km) and maximum velocity (Vmax) using a Hanes plot. Km and Vmax values were found to be higher in the measurement at pH 7.0 and 75oC. The Km values at four different combinations of pH and temperatures (pH 6.5, 40oC; pH 6.5, 75oC; pH 7.0, 40oC; pH 7.0, 75oC) were found to be 0.86, 3.23, 0.77 and 3.83 mg/mL, respectively; and Vmax values were 17.5, 54.3, 20.3 and 57.1 µg/mL/min, respectively. The results obtained showed that hydrolysis rate of this starch was somewhat low.

Download Full-text