Simultaneous quantification and pharmacokinetic investigation of selexipag and its main metabolite ACT-333679 in rat plasma by UPLC-MS/MS method

2020 ◽  
Vol 190 ◽  
pp. 113496
Author(s):  
Saili Xie ◽  
Lu Shi ◽  
Jingjing Chen ◽  
Ren-ai Xu ◽  
Xuemei Ye
Keyword(s):  
Rat Plasma ◽  
Main Metabolite ◽  
Simultaneous Quantification ◽  
Pharmacokinetic Investigation

scholarly journals LC-MS/MS Method for Simultaneous Quantification of Dexmedetomidine, Dezocine, and Midazolam in Rat Plasma and Its Application to Their Pharmacokinetic Study

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Wenjuan Cui ◽  
Qin Liu ◽  
Shan Xiong ◽  
Lujun Qiao
Keyword(s):  
Storage Conditions ◽  
Rat Plasma ◽  
Selected Reaction Monitoring ◽  
Pharmacokinetic Studies ◽  
Reaction Monitoring ◽  
Simultaneous Quantification ◽  
Ionization Mode ◽  
The Stability ◽  
Lower Limits ◽  
Interday Precision

A simple, sensitive, and accurate LC-MS/MS method was established and validated for the simultaneous quantification of dexmedetomidine, dezocine, and midazolam in rat plasma. Chromatographic separation was achieved on a C18 column (50 mm × 2.1 mm, 3 µm) using a mobile phase composed of water (containing 0.1% formic acid) and acetonitrile. The lower limits of quantification were 0.1, 0.1, and 0.2 ng/mL for dexmedetomidine, dezocine, and midazolam in rat plasma, respectively. The analytes were determined with selected reaction monitoring under positive ionization mode. The intra- and interday precision and accuracy were all within acceptable limits during the entire validation, and the stability of analytes was acceptable under various storage conditions. The validated method was successfully applied in pharmacokinetic studies of dexmedetomidine, dezocine, and midazolam following intravenous injection.

scholarly journals A simple sensitive UFLC-MS/MS method for the simultaneous quantification of artesunate, dihydroartemisinin and quercetin in rat plasma and its application to pharmacokinetic studies

RSC Advances ◽  
2019 ◽  
Vol 9 (71) ◽  
pp. 41794-41802
Author(s):  
Nethravathi Puttappa ◽  
Karthik Yamjala ◽  
Narenderan S. T. ◽  
Suresh Kumar Raman ◽  
Gowthamarajan Kuppusamy ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Active Metabolite ◽  
Rat Plasma ◽  
Simultaneous Estimation ◽  
Pharmacokinetic Studies ◽  
Tandem Mass ◽  
Simultaneous Quantification ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

An ultrafast liquid chromatography-tandem mass spectrometry (UFLC-MS/MS) method was developed for the simultaneous estimation of artesunate (ART), dihydroartemisinin (DHA, an active metabolite of ART) and quercetin (QRT) in rat plasma.

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