Improved Neurodevelopmental Outcomes Associated with Bovine Milk Fat Globule Membrane and Lactoferrin in Infant Formula: A Randomized, Controlled Trial

Abstract Objectives Bovine milk fat globule membrane (bMFGM) added in routine infant formula supports normal growth and safety through 24 mo of age in term infants. The impact on micronutrients and glucose-related biomarkers is assessed here. Methods In this double-blind, randomized, controlled trial, formula-fed infants were enrolled (<120 days of age) and randomized to receive a standard cow´s milk-based infant formula (SF, n = 174) or a similar formula with added whey protein-lipid concentrate (5 g/L; bMFGM; EF n = 173); exclusively breastfed infants were enrolled as a reference (HM, n = 235). In 50% of infants (chosen at random), parents agreed to blood collection (2–4 h fasting) at baseline, 6, 12, and 24 mo of age. Serum micronutrients (zinc, iron, ferritin, transferrin receptor) and glucose-related biomarkers (glucose, insulin, HOMA-IR [Homeostatic Model Assessment-Insulin Resistance], IGF-1) were analyzed. Results At baseline, significant differences in markers of iron status (serum iron, ferritin) and glucose-related biomarkers (glucose, HOMA-IR, and IGF-1) were detected between HM and study formula groups. At 6 and 12 mo, no differences in any measure were detected between study formula groups. Serum iron and ferritin at 12 mo as well as glucose-related biomarkers at 6 mo (insulin, HOMA-IR, IGF-1) and 12 mo (IGF-1 only) were lower in the HM vs study formula groups. By 24 mo, micronutrients and glucose-related biomarkers were similar between study formula groups (with the exception of significantly lower serum iron in the SF group); for HM vs study formula groups, differences included significantly lower zinc and IGF-1. Conclusions Patterns of micronutrients and glucose-related biomarkers were similar through two years of age in infants who received formula through one year. This study add to the body of data available for glucose-related biomarkers in children at two years of age and younger. Funding Sources The study was funded by Mead Johnson Nutrition (MJN). TMP, SSW, and JLW are currently, and CLB was previously employed by MJN.

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Growth Through 24 Months of Age in Infants Receiving Formulas with or Without Added Bovine Milk Fat Globule Membrane (MFGM) or Human Milk Through the First Year of Life: An RCT

Current Developments in Nutrition ◽

10.1093/cdn/nzaa054_086 ◽

2020 ◽

Vol 4 (Supplement_2) ◽

pp. 1014-1014

Author(s):

Angela Jaramillo Ospina ◽

Rosario Toro ◽

Teresa Murguia-Peniche ◽

Jennifer Wampler ◽

Steven Wu ◽

...

Keyword(s):

Human Milk ◽

Infant Formula ◽

Milk Fat ◽

Reference Group ◽

Bovine Milk ◽

Study Time ◽

Milk Fat Globule Membrane ◽

Milk Fat Globule ◽

Z Scores ◽

Fat Globule

Abstract Objectives To evaluate growth through 24 months of age in infants receiving added bovine milk fat globule membrane (MFGM) in infant formula through 12 months of age. Concentration of MFGM from bovine milk fractions and incorporation in infant formula may better approximate the composition of complex milk lipids in human milk. Methods In the double-blind, randomized, controlled Chilean Infant Nutrition Trial (ChiNuT; NCT0262613), term infants whose mothers chose to initiate exclusive infant formula feeding before 4 months of age were randomized to receive: a standard cow's milk-based infant formula (SF, n = 174) or a similar formula with added whey protein-lipid concentrate (5 g/L; source of bovine MFGM) (bMFGM, n = 176). A reference group of infants exclusively receiving human milk (HM, n = 236) was also recruited. Growth through 24 months of age was the primary outcome. Length-for-age (LAZ), weight-for-age (WAZ) and body mass index (BMI)-for-age (BAZ) growth z-scores were analyzed by mixed-effects multiple linear regression models adjusted by sex, age (days), and maternal pregestational BMI (kg/m2). Results No significant group differences in sex, gestational age at birth, birthweight, maternal age and maternal education were detected, with the exception of maternal pregestational BMI (mean(SD)) (HM: 27.0(5.2) lower vs SF: 28.6(6.2) or bMFGM: 28.9(6.1); P = 0.002). Groups were similar at baseline (weight, length, WAZ, BAZ) with the exception of LAZ (lower in the bMFGM compared to HM group; P < 0.05). No significant differences in growth z-scores (absolute at 6, 9, 12, and 24 months of age or change between baseline and each study time point) were detected between SF and MFGM groups. Both randomized study formula groups were associated with higher growth z-score increases from baseline compared to the HM reference group between 6 and 24 months (P less than 0.05). Mean growth z-scores fell within the range of −1 to 1 (16th to 84th percentile) for SF, bMFGM, and HM groups at all study time points. Conclusions Added bovine MFGM in a routine cow's milk-based infant formula through 12 months of age supported normal growth through 24 months of age. Funding Sources The study was funded by Mead Johnson Nutrition (MJN). Teresa Murguia-Peniche, Steven Wu, and Jennifer Wampler are currently employed by MJN. Carol Lynn Berseth was previously employed by MJN.

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Supplementation with milk enriched with complex lipids during pregnancy: A double-blind randomized controlled trial

PLoS ONE ◽

10.1371/journal.pone.0244916 ◽

2021 ◽

Vol 16 (2) ◽

pp. e0244916

Author(s):

Benjamin B. Albert ◽

José G. B. Derraik ◽

Yin-Yin Xia ◽

Tom Norris ◽

Ting Zhang ◽

...

Keyword(s):

Clinical Trial ◽

Milk Fat ◽

Reference Group ◽

Controlled Trial ◽

Double Blind ◽

Milk Fat Globule Membrane ◽

Randomized Controlled ◽

Milk Fat Globule ◽

Fat Globule ◽

Enriched Milk

Background Gangliosides are a class of sphingolipids that are present in the cell membranes of vertebrates. Gangliosides influence a broad range of cellular processes through effects on signal transduction, being found abundantly in the brain, and having a role in neurodevelopment. Objective We aimed to assess the effects of maternal daily consumption of ganglioside-enriched milk vs non-enriched milk and a non-supplemented group of pregnant women on maternal ganglioside levels and pregnancy outcomes. Design Double-blind parallel randomized controlled trial. Methods 1,500 women aged 20–40 years were recruited in Chongqing (China) between 11 and 14 weeks of a singleton pregnancy, and randomized into three groups: Control–received standard powdered milk formulation (≥4 mg gangliosides/day); Complex milk lipid-enhanced (CML-E) group–same formulation enriched with complex milk lipids (≥8 mg gangliosides/day) from milk fat globule membrane; Reference–received no milk. Serum ganglioside levels were measured in a randomly selected subsample of 250 women per group. Results CML-E milk was associated with marginally greater total gangliosides levels in maternal serum compared to Control (13.02 vs 12.69 μg/ml; p = 0.034) but not to Reference group. CML-E milk did not affect cord blood ganglioside levels. Among the 1500 women, CML-E milk consumption was associated with a lower rate of gestational diabetes mellitus than control milk [relative risk 0.80 (95% CI 0.64, 0.99)], but which was not different to the Reference group. CML-E milk supplementation had no other effects on maternal or newborn health. Conclusions Maternal supplementation with milk fat globule membrane, as a source of gangliosides, was not associated with any adverse health outcomes, and did not increase serum gangliosides compared with the non-supplemented reference group. Trial registration Chinese Clinical Trial Register (ChiCTR-IOR-16007700). Clinical trial registration ChiCTR-IOR-16007700; www.chictr.org.cn/showprojen.aspx?proj=12972.

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Comparison of bovine milk fat globule membrane protein retention by different ultrafiltration membranes using a label-free proteomic approach

LWT ◽

10.1016/j.lwt.2021.111219 ◽

2021 ◽

pp. 111219

Author(s):

Mengqi Wang ◽

Chunjie Cao ◽

Yi Wang ◽

Hongbo Li ◽

Hongjuan Li ◽

...

Keyword(s):

Membrane Protein ◽

Milk Fat ◽

Bovine Milk ◽

Label Free ◽

Ultrafiltration Membranes ◽

Milk Fat Globule Membrane ◽

Protein Retention ◽

Proteomic Approach ◽

Milk Fat Globule ◽

Fat Globule

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Production and characterization of monoclonal antibodies directed against bovine milk fat globule membrane (MFGM)

Biochimica et Biophysica Acta (BBA) - General Subjects ◽

10.1016/0304-4165(94)90101-5 ◽

1994 ◽

Vol 1199 (1) ◽

pp. 87-95 ◽

Cited By ~ 20

Author(s):

Naohito Aoki ◽

Hidenori Kuroda ◽

Miho Urabe ◽

Yoshimi Taniguchi ◽

Takahiro Adachi ◽

...

Keyword(s):

Monoclonal Antibodies ◽

Milk Fat ◽

Bovine Milk ◽

Milk Fat Globule Membrane ◽

Milk Fat Globule ◽

Fat Globule

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Chemical changes in bovine milk fat globule membrane caused by heat treatment and homogenization of whole milk

Journal of Dairy Research ◽

10.1017/s002202990200571x ◽

2002 ◽

Vol 69 (4) ◽

pp. 555-567 ◽

Cited By ~ 83

Author(s):

SUNG JE LEE ◽

JOHN W. SHERBON

Keyword(s):

Heat Treatment ◽

Membrane Proteins ◽

Milk Fat ◽

Whey Proteins ◽

Bovine Milk ◽

Chemical Changes ◽

Milk Fat Globule Membrane ◽

Whole Milk ◽

Milk Fat Globule ◽

Fat Globule

The effects of heat treatment and homogenization of whole milk on chemical changes in the milk fat globule membrane (MFGM) were investigated. Heating at 80 °C for 3–18 min caused an incorporation of whey proteins, especially β-lactoglobulin (β-lg), into MFGM, thus increasing the protein content of the membrane and decreasing the lipid. SDS-PAGE showed that membrane glycoproteins, such as PAS-6 and PAS-7, had disappeared or were weakly stained in the gel due to heating of the milk. Heating also decreased free sulphydryl (SH) groups in the MFGM and increased disulphide (SS) groups, suggesting that incorporation of β-lg might be due to association with membrane proteins via disulphide bonds. In contrast, homogenization caused an adsorption of caseins to the MFGM but no binding of whey proteins to the MFGM without heating. Binding of caseins and whey proteins and loss of membrane proteins were not significantly different between milk samples that were homogenized before and after heating. Viscosity of whole milk was increased when milk was treated with both homogenization and heating.

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