An overview of structural features of DNA and RNA complexes with saffron compounds: Models and antioxidant activity

2009 ◽  
Vol 95 (3) ◽  
pp. 204-212 ◽  
Author(s):  
C.D. Kanakis ◽  
P.A. Tarantilis ◽  
C. Pappas ◽  
J. Bariyanga ◽  
H.A. Tajmir-Riahi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Structural Features ◽  
Dna And Rna ◽  
Rna Complexes

DNA-RNA complexes that might represent transient attachment sites of nuclear DNA to the matrix

1990 ◽  
Vol 95 (4) ◽  
pp. 667-674
Author(s):  
C. Patriotis ◽  
M. Andreeva ◽  
M. Pascaleva ◽  
V. Ivanov ◽  
L. Djondjurov
Keyword(s):  
Dna Sequences ◽  
Nuclear Dna ◽  
Rna Polymerase Iii ◽  
Erythroid Differentiation ◽  
Rnase H ◽  
Dna And Rna ◽  
Attachment Sites ◽  
The Matrix ◽  
Exo Iii ◽  
Rna Complexes

In this study we describe DNA-RNA complexes in matrix DNA of Friend cells. The presence of such unusual structures is confirmed by the following evidence. When a preparation of matrix DNA is electrophoresed in agarose an RNA component always migrates together with DNA. There should be a close interaction between DNA and RNA in such a preparation because the presence of the RNA component causes resistance of DNA to DNase I and Exo III. An intimate, hybrid-type association of part of the RNA component with DNA is indicated also by the fact that about 20% of this RNA is sensitive to RNase H. By specific inhibition of the RNA synthesis with alpha-amanitin and actinomycin D it was shown that the bulk of associated RNA is transcribed by RNA polymerase III. Hybridization experiments showed similarity between the DNA sequences isolated from the complexes and those from the base of dehistonized DNA loops obtained by high-salt extraction of nuclei. This observation suggests that the complexes might represent attachment sites of nuclear DNA to the matrix: possibly, the attachment is mediated via the RNA component. Experiments with induction of erythroid differentiation indicated that a profound reorganization of the nucleus, accompanying terminal differentiation, leads to a striking reduction in the number of complexes and thus in the number of attachment sites. This suggests that the complexes should function as transient attachment sites.

scholarly journals Structural Characterization and Antioxidant Activity of Milled Wood Lignin from Xylose Residue and Corncob

Polymers ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 2092 ◽  
Author(s):  
Miaomiao Xu ◽  
Chao Wang ◽  
Gaojin Lyu ◽  
Lei Zhong ◽  
Liyuan Yang ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Structural Changes ◽  
Food Additives ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Gel Permeation Chromatography ◽  
Structural Features ◽  
2D Nmr Spectroscopy ◽  
Milled Wood Lignin ◽  
Xylose Residue

Xylose residue (XR), after diluted acid treatment of corncob, consists of cellulose and lignin. However, structural changes of XR lignin have not been investigated comprehensively, and this has seriously hindered the efficient utilization of lignin. In this study, corncob milled wood lignin (CC MWL), and xylose residue milled wood lignin (XR MWL) were isolated according to the modified milled wood lignin (MWL) method. The structural features of two lignin fractions were thoroughly investigated via fourier transform infrared spectroscopy (FTIR), gel permeation chromatography (GPC), thermogravimetric analysis (TGA) and two dimensional nuclear magnetic resonance (2D NMR) spectroscopy techniques. XR MWL with higher yield and lower bound carbohydrate contents presented more phenolic OH contents than CC MWL due to partial cleavage of β-O-4. Furthermore, the molecular weights of XR MWL were increased, possibly because of condensation of the lignin during the xylose production. A study on antioxidant activity showed that XR lignin had better radical scavenging ability than that of 2,6-Di-tert-butyl-4-methyl-phenol (BHT) and CC MWL. The results suggested that the lignin in xylose residue, showing great antioxidant properties, has potential applications in food additives.

Structural features and antioxidant activity of a new galactoglucan from edible mushroom Pleurotus djamor

2020 ◽  
Author(s):  
Gajendra Nath Maity ◽  
Prasenjit Maity ◽  
Somanjana Khatua ◽  
Krishnendu Acharya ◽  
Sudipta Dalai ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Edible Mushroom ◽  
Structural Features ◽  
Pleurotus Djamor

scholarly journals Antioxidant Activity of Selected Phenolic Acids–Ferric Reducing Antioxidant Power Assay and QSAR Analysis of the Structural Features

Molecules ◽  
2020 ◽  
Vol 25 (13) ◽  
pp. 3088
Author(s):  
Maciej Spiegel ◽  
Karina Kapusta ◽  
Wojciech Kołodziejczyk ◽  
Julia Saloni ◽  
Beata Żbikowska ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Acids ◽  
Density Functional ◽  
Multiple Linear Regression Model ◽  
Structural Features ◽  
Hydroxyl Groups ◽  
Antioxidant Power ◽  
Qsar Analysis ◽  
Structure Activity ◽  
Ferric Reducing Antioxidant Power

Phenolic acids are naturally occurring compounds that are known for their antioxidant and antiradical activity. We present experimental and theoretical studies on the antioxidant potential of the set of 22 phenolic acids with different models of hydroxylation and methoxylation of aromatic rings. Ferric reducing antioxidant power assay was used to evaluate this property. 2,3-dihydroxybenzoic acid was found to be the strongest antioxidant, while mono hydroxylated and methoxylated structures had the lowest activities. A comprehensive structure–activity investigation with density functional theory methods elucidated the influence of compounds topology, resonance stabilization, and intramolecular hydrogen bonding on the exhibited activity. The key factor was found to be a presence of two or more hydroxyl groups being located in ortho or para position to each other. Finally, the quantitative structure–activity relationship approach was used to build a multiple linear regression model describing the dependence of antioxidant activity on structure of compounds, using features exclusively related to their topology. Coefficients of determination for training set and for the test set equaled 0.9918 and 0.9993 respectively, and Q2 value for leave-one-out was 0.9716. In addition, the presented model was used to predict activities of phenolic acids that haven’t been tested here experimentally.

scholarly journals Comparison of structural features and antioxidant activity of polysaccharides from natural and cultured Cordyceps sinensis

2017 ◽  
Vol 26 (1) ◽  
pp. 55-62 ◽  
Author(s):  
Junqiao Wang ◽  
Shaoping Nie ◽  
Lijiao Kan ◽  
Haihong Chen ◽  
Steve W. Cui ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Structural Features ◽  
Cordyceps Sinensis

scholarly journals GeTallele: a method for integrative analysis and visualization of DNA and RNA allele frequencies

2018 ◽  
Author(s):  
Piotr Słowiński ◽  
Muzi Li ◽  
Paula Restrepo ◽  
Nawaf Alomran ◽  
Liam F. Spurr ◽  
...  
Keyword(s):  
Gene Dosage ◽  
Transcriptional Response ◽  
Structural Features ◽  
Integrative Analysis ◽  
Allele Frequencies ◽  
Biological Traits ◽  
Specific Expression ◽  
Dna And Rna ◽  
Wide Range ◽  
Rna And Dna

AbstractBackgroundAsymmetric allele expression typically indicates functional and/or structural features associated with the underlying genetic variants. When integrated, RNA and DNA allele frequencies can reveal patterns characteristic of a wide-range of biological traits, including ploidy changes, genome admixture, allele-specific expression and gene-dosage transcriptional response.ResultsTo assess RNA and DNA allele frequencies from matched sequencing datasets, we introduce a method for generating model distributions of variant allele frequencies (VAF) with a given variant read probability. In contrast to other methods, based on whole sequences or single SNV, proposed methodology uses continuous multi-SNV genomic regions. The methodology is implemented in a GeTallele toolbox that provides a suite of functions for integrative analysis, statistical assessment and visualization of Genome and Transcriptome allele frequencies. Using model VAF probabilities, GeTallele allows estimation and comparison of variant read probabilities (VAF distributions) in a sequencing dataset. We demonstrate this functionality across cancer DNA and RNA sequencing datasets.ConclusionBased on our evaluation, variant read probabilities can serve as a dependable indicator to assess gene and chromosomal allele asymmetries and to aid calls of genomic events in matched sequencing RNA and DNA [email protected]

Hydroxycinnamic acid bound arabinoxylans from millet brans-structural features and antioxidant activity

2016 ◽  
Vol 88 ◽  
pp. 296-305 ◽  
Author(s):  
Vandana Bijalwan ◽  
Usman Ali ◽  
Atul Kumar Kesarwani ◽  
Kamalendra Yadav ◽  
Koushik Mazumder
Keyword(s):  
Antioxidant Activity ◽  
Structural Features ◽  
Hydroxycinnamic Acid
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