Formulation and characterization of propolis and tea tree oil nanoemulsion loaded with clindamycin hydrochloride for wound healing: In-vitro and in-vivo wound healing assessment

O interesse por medicamentos alternativos, principalmente daqueles provenientes de extratos naturais, tem aumentado nas últimas décadas. A Melaleuca alternifolia é um arbusto pertencente ao gênero Melaleuca, popularmente conhecida como "árvore de chá", cujo principal produto é o óleo essencial (TTO - tea tree oil), de grande importância medicinal por possuir comprovada ação bactericida e antifúngica contra diversos patógenos humanos. Em virtude da atividade terapêutica em diversas especialidades médicas, o TTO passou a ser empregado na área odontológica. Esta revisão de literatura foi realizada com o objetivo de discutir os ensaios já realizados com o TTO contra microrganismos relacionados à doença cárie, doença periodontal e problemas pulpares. O óleo de Melaleuca tem demonstrado boa ação antibacteriana in vitro contra microrganismos bucais, porém, pesquisas envolvendo o estudo do mecanismo de ação sobre as células microbianas ou estudos in vivo ainda são escassos e precisam ser realizados, já que esse produto pode ser útil na odontologia, seja na manutenção química da higiene ou prevenção de doenças bucais.

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HERBAL CREAMS OF REISHI EXTRACT AND TEA TREE OIL FOR HIRSUTISM–AN IN VIVO STUDY

International Journal of Current Pharmaceutical Research ◽

10.22159/ijcpr.2020v12i5.39781 ◽

2020 ◽

pp. 106-110

Author(s):

SANGEETA CHOUDHURY ◽

BLR MADHAVI

Keyword(s):

Topical Application ◽

Hair Growth ◽

Ethanolic Extract ◽

Hair Follicles ◽

In Vivo Studies ◽

Control Group ◽

Tea Tree Oil ◽

Tea Tree

Objective: The aim of this work to formulate, evaluate and compare the effectiveness of herbal creams containing extract of reishi and tea tree oil for treating hirsutism. Methods: Herbal ingredients were authenticated. Cream base was initially formulated. Three formulations of herbal cream were prepared. Reishi ethanolic extract, tea tree oil, and combination of tea tree oil and reishi extract were added to the cream base and formulated cream were named as RHC, THC and RTC respectively. In vitro evaluations on herbal creams were done for the physicochemical characteristics. In vivo studies were carried out on female Swiss Albino mice for the activity against hair growth by topical application of cream to shaved skin. The histological and morphometric evaluation was carried out. Skin irritancy study was conducted. Results: The herbal creams showed desirable physicochemical properties like pH, viscosity and spreadability. Statistical analysis for the length of hair was performed by using one way ANOVA followed by DUNNET’S post hoc test where THC and RTC were found to be significant whereas RHC showed no significant reduction of hair growth compared to control. RTC showed a significant effect at p<0.05 and hair growth reduction was significant for THC at p<0.001 compared to the control group. RTC and THC showed mild to moderate reduction in the size of the hair follicles with a reduction of sebaceous gland size in the histological analysis. Conclusion: Topical application of herbal creams to mice showed that hair growth was fastest in group RHC and was slowest in group THC and intermediate with RTC. It can be concluded that these herbal actives can be used as an effective treatment against hirsutism. Within the study period, tea tree oil was found to be more effective than reishi extract and the combination product. Further formulation studies and in vivo studies need to be carried out on reishi to assess its effectiveness against hirsutism.

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Formulation and in vitro characterization of tea tree oil anti-dandruff shampoo

Current Cosmetic Science ◽

10.2174/2666779701666210426085302 ◽

2021 ◽

Vol 01 ◽

Author(s):

Humra Umar ◽

Tariq Mahmood ◽

Talib Hussain ◽

Rabia Aslam ◽

Yasser Shahzad ◽

...

Keyword(s):

Salicylic Acid ◽

Antifungal Activity ◽

Synergistic Effects ◽

Quality Attributes ◽

Tea Tree Oil ◽

Tea Tree ◽

In Vitro Characterization ◽

Selenium Sulfide

Background: Dandruff is the common scalp condition affecting half of the population of the world. Objective: The current study was aimed at developing anti-dandruff shampoos containing tea tree oil, which is believed to have effects against Malassezia furfur, a fungus involved in dandruff production. Methodology: Various shampoos containing tea tree oil in 0.5 to 3% concentration were prepared after careful selection of various shampoo ingredients. The formulated shampoos were subjected to various quality tests such as pH, viscosity, foam production, dirt dispersion, wetting time, surface tension, solid contents and antimicrobial activity against a model fungal strain, namely Candida albicans. The formulated shampoos were also compared with the marketed shampoos for quality attributes. Results: The results revealed that tea tree oil shampoos had pH values in the range of 5 – 6, which is close to the slightly acidic skin’s pH and considered as good for hair. All other quality attributes were comparable to the marketed products. The marketed shampoos had superior antifungal activity due to presence of zinc pyrithione or higher concentration of salicylic acid or selenium sulfide. Notwithstanding, the tea tree oil shampoos demonstrated an appreciable antifungal activity due to synergistic effects of tea tree oil, sodium lauryl sulphate and salicylic acid. Furthermore, the tea tree oil shampoos were stable during two months long stability testing. Conclusion: Thus, tea tree oil anti-dandruff shampoos have the potential to address the dandruff problem.

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Effects and possible mechanism of tea tree oil against Botrytis cinerea and Penicillium expansum in vitro and in vivo test

Canadian Journal of Microbiology ◽

10.1139/cjm-2016-0553 ◽

2017 ◽

Vol 63 (3) ◽

pp. 219-227 ◽

Cited By ~ 12

Author(s):

Yonghua Li ◽

Xingfeng Shao ◽

Jiayu Xu ◽

Yingying Wei ◽

Feng Xu ◽

...

Keyword(s):

Cell Membrane ◽

Botrytis Cinerea ◽

Total Lipid ◽

Penicillium Expansum ◽

In Vitro Test ◽

Respiratory Metabolism ◽

Tea Tree Oil ◽

Tea Tree

The purpose of this study was to investigate the antifungal activities and possible mechanisms of tea tree oil (TTO) against Botrytis cinerea and Penicillium expansum in vitro and in vivo. The results show that TTO exhibits dose-dependent antifungal activity against both pathogens, but P. expansum is less sensitive than B. cinerea to TTO not only in the in vitro test but also in artificially inoculated cherry fruits. TTO vapor treatment reduced the decay caused by these pathogens in inoculated cherry fruits, but the effect on P. expansum was less than that on B. cinerea. While the total lipid and ergosterol contents of the cell membrane are greater in P. expansum than in B. cinerea, TTO treatment lowers the total lipid content in the membranes of both species by well over 50%, and ergosterol content is reduced to a greater extent in B. cinerea than in P. expansum. In both pathogens, TTO alters mycelial morphology and cellular ultrastructure. Oxygen consumption measurements show that TTO inhibits respiratory metabolism via the tricarboxylic acid cycle pathway in both pathogens, though more severely in B. cinerea than in P. expansum. The relatively decreased sensitivity of P. expansum to TTO may be due to the fact that TTO causes less disruption of the cell membrane in this organism, and higher inhibition the respiratory metabolism to the extent observed in B. cinerea.

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In vitro and in vivo toxicity of several fungicides and Timorex gold biofungicide to Pythuim aphanidermatum

Pesticidi i fitomedicina ◽

10.2298/pif1302117m ◽

2013 ◽

Vol 28 (2) ◽

pp. 117-123 ◽

Cited By ~ 5

Author(s):

Milica Mihajlovic ◽

Emil Rekanovic ◽

Jovana Hrustic ◽

Brankica Tanovic ◽

Ivana Potocnik ◽

...

Keyword(s):

Untreated Control ◽

Control Plot ◽

Tea Tree Oil ◽

Greenhouse Soil ◽

In Vivo Toxicity ◽

Tea Tree ◽

Better Than

A survey of in vitro and in vivo sensitivity of Pythuim aphanidermatum to several commercial fungicides and a biofungicide was undertaken. An isolate of P. aphanidermatum pathogenic to pepper was collected from a naturally infested greenhouse soil from Smederevska Palanka, Serbia. The P. aphanidermatum isolate was sensitive to all tested products. The obtained EC50 values were as follows: 10.21 mg/l for propamocarb-hydrochloride, 302.65 mg/l for fosetyl-Al, 11.18 mg/l for mancozeb, 1.27 mg/l for mefenoxam, 0.05 mg/l for azoxystrobin, and 175.33 mg/l for tea tree oil. Under greenhouse conditions, fosetyl-Al was the most efficient fungicide among the tested substances (97.5%). The biofungicide tea tree oil (Timorex Gold) (35.0%) exhibited the lowest efficacy among the tested materials, but it was still significantly better than the untreated control plot. The efficacies of propamocarb-hydrochloride (Previcur 607 SL), mancozeb (Mankogal 80 WP), azoxystrobin (Quadris) and mefenoxam (Ridomil gold 480 SL), were 72.5%, 77.5%, 57.5% and 75.0%, respectively.

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Preparation and characterization of succinyl chitosan and succinyl chitosan nanoparticle film: In vitro and in vivo evaluation of wound healing activity

International Journal of Biological Macromolecules ◽

10.1016/j.ijbiomac.2021.11.015 ◽

2021 ◽

Author(s):

N.T. Thu Thao ◽

H.M.S.M. Wijerathna ◽

R. Saravana Kumar ◽

Dongrack Choi ◽

S.H.S. Dananjaya ◽

...

Keyword(s):

Wound Healing ◽

In Vivo Evaluation ◽

Chitosan Nanoparticle ◽

Wound Healing Activity ◽

Nanoparticle Film

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Spectroscopic Characterization of a Polycaprolactone-Chitosan Electrospun Scaffold Modified with Photocatalytic TiO2 Nanoparticles for Improved Wound Healing: A Complete In Vivo Evaluation

Journal of Biomedical Nanotechnology ◽

10.1166/jbn.2021.3079 ◽

2021 ◽

Vol 17 (5) ◽

pp. 889-900

Author(s):

Cuizhen Sun ◽

Dianju He ◽

Yonghua Qi ◽

Guiqin Zhang ◽

Qiujin Huang

Keyword(s):

Wound Healing ◽

Lattice Structure ◽

Spectroscopic Characterization ◽

Electrospun Scaffold ◽

Zone Of Inhibition ◽

In Vivo Evaluation ◽

Electrospinning Technique

In the current study, we hypothesized that the electrospun scaffold chitosan (CS)/polycaprolactone (PCL)/titanium dioxide (TiO2) could be prepared by combining CS, PCL, and TiO2 nanoparticles (TiO2 NPs) using an electrospinning technique for wound dressing applications. The CS/PCL/TiO2 electrospun scaffold was prepared and characterized by UV-Vis, SEM, TEM, FTIR, and XRD analyses. Based on the UV-Vis analysis, the incorporation of CS/PCL on the surface of TiO2 NPs affected their optical properties. Further, CS/PCL and CS/PCL/TiO2 were found to have uniform distribution in fiber diameter with no bead morphology, as confirmed by SEM. The XRD spectrum of the CS/PCL/TiO2 revealed that the TiO2 NPs were adequately mixed with the CS/PCL solution, exhibiting the planes of TiO2 peaks (112), (105), (204), (116), and (301), which aligned well with the lattice structure. The antibacterial activity of CS/PCL/TiO2 against Staphylococcus aureus and Escherichia coli was evaluated using the zone of inhibition method. By testing the cytocompatibility of CS/PCL/TiO2 in vitro, this dressing was found to have a less toxic nature. In addition, In Vivo wound healing studies showed that the dressing prepared with the CS/PCL/TiO2 electrospun scaffold improved wound healing compared to that prepared with CS/PCL alone. The above results strongly support the use of CS/PCL/TiO2 electrospun scaffold as an effective dressing for wound healing.

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