130. Trypsin Stimulates Mitogen Activated Protein Kinases and Cell Growth in Barrett’S Esophagus

2008 ◽  
Vol 144 (2) ◽  
pp. 233
Author(s):  
Cuc T. Tran ◽  
Caitlin B. Peterson ◽  
George A. Sarosi
Keyword(s):  
Cell Growth ◽  
Barrett’S Esophagus ◽  
Protein Kinases ◽  
Barrett's Esophagus ◽  
Mitogen Activated Protein Kinases ◽  
Mitogen Activated Protein

Activity of mitogen-activated protein kinases in the esophageal epithelium of patients with Barrett's esophagus

2014 ◽  
Vol 28 (6) ◽  
pp. 585-592 ◽  
Author(s):  
A. Chwiesko ◽  
A. Baniukiewicz ◽  
J. Semeniuk ◽  
M. Kaczmarski ◽  
J. Wasielica-Berger ◽  
...  
Keyword(s):  
Barrett’S Esophagus ◽  
Protein Kinases ◽  
Barrett's Esophagus ◽  
Esophageal Epithelium ◽  
Mitogen Activated Protein Kinases ◽  
Mitogen Activated Protein

Mitogen-activated protein kinases control p27/Kip1 expression and growth of human melanoma cells

2001 ◽  
Vol 357 (1) ◽  
pp. 297-303 ◽  
Author(s):  
Marcin KORTYLEWSKI ◽  
Peter C. HEINRICH ◽  
Maria-Elisabeth KAUFFMANN ◽  
Markus BÖHM ◽  
Andrzej MACKIEWICZ ◽  
...  
Keyword(s):  
Cell Growth ◽  
Protein Kinases ◽  
Melanoma Cells ◽  
Human Melanoma ◽  
Mitogen Activated Protein Kinases ◽  
Mapk Activity ◽  
Human Melanoma Cells ◽  
Mitogen Activated Protein ◽  
P27 Kip1 ◽  
Constitutively Active

The mitogen-activated protein kinases (MAPKs) extracellular signal-regulated protein kinase (ERK)1 and ERK2, involved in regulating cell growth and differentiation, are constitutively active in A375 and WM239 human melanoma cells. Using PD098059, an inhibitor of MAPK kinase (MEK), we investigated the role of persistently activated ERK1/2 in cell growth. The inhibition of MAPK activity induced a dose-dependent growth arrest in G0/G1 phase. Correspondingly, we observed the up-regulation of the cyclin-dependent kinase (Cdk) inhibitor p27/Kip1 and hypophosphorylation of the retinoblastoma protein. Further studies showed that PD098059 treatment significantly decreased Cdk2 kinase activity, most probably owing to an augmented level of p27/Kip1 associated with cyclin E–Cdk2 complexes. The accumulation of p27/Kip1 protein in A375 cells was attributed to its increased stability. Our findings suggest that constitutively active ERK1/2 kinases contribute to the growth of melanoma cells by negative regulation of the p27/Kip1 inhibitor.

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