Fat body catalase activity as a biochemical index for the recognition of thermotolerant breeds of mulberry silkworm, Bombyx mori L.

2011 ◽  
Vol 36 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Pezhman Nabizadeh ◽  
T.S. Jagadeesh Kumar
2002 ◽  
Vol 80 (8) ◽  
pp. 1451-1459 ◽  
Author(s):  
Natraj Krishnan ◽  
Anathbandhu Chaudhuri

Changes in tissue-specific NADP-dependent malate dehydrogenase (MDH) activity and protein and cholesterol contents and retardation of growth and development in the mulberry silkworm, Bombyx mori L., induced by infection with a baculovirus, the B. mori nucleopolyhedrovirus (BmNPV), were investigated. The study revealed that the relative growth rate and development of the fifth-instar larva was significantly inhibited during the progression of nuclear polyhedrosis disease at various times post inoculation (p.i.). Percent pupation and reproductive output were also adversely affected. NADP-dependent MDH activity in the hemolymph peaked sharply at 6 h p.i. and then gradually decreased, reaching a minimum at 264 h p.i., with exceptions at 30 and 72 h p.i. These levels of MDH activity were different from those in non-infected controls. In fat-body tissues, NADP-dependent MDH activity was significantly higher in infected insects than in non-infected controls of the same age. These results indicate that baculovirus infection causes significant changes in intermediary metabolic pathways, causing a significant fall and rise in protein and cholesterol contents in tissues during development of fifth-instar larvae.


Foods ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 871 ◽  
Author(s):  
Artorn Anuduang ◽  
Yuet Ying Loo ◽  
Somchai Jomduang ◽  
Seng Joe Lim ◽  
Wan Aida Wan Mustapha

The mulberry silkworm (Bombyx mori L.) is a common edible insect in many countries. However, the impact of thermal processing, especially regarding Thai silkworm powder, is poorly known. We, therefore, determined the optimum time for treatment in hot water and subsequent drying temperatures in the production of silkworm powder. The silkworms exposed to 90 °C water for 0, 5, 10, 15, and 20 min showed values of Total Phenolic Compounds (TPCs), 2,2-Diphenyl-1-picrylhydrazyl free radical scavenging (DPPH) assay, 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay, and Ferric Reducing Antioxidant Power (FRAP) assay that were significantly (p < 0.05) higher at the 5 min exposure time compared with the other times. The reduction of microorganisms based on log CFU/g counts was ≥3 log CFU/g (99%) at the 5 min treatment. To determine the optimum drying temperature, the silkworms exposed to 90 °C water for 5 min were subjected to a hot-air dryer at 80, 100, 120, and 140 °C. The TPC value was the highest (p < 0.05) at 80 °C. The silkworm powder possessed significantly (p < 0.05) higher DPPH, ABTS radical scavenging ability, and ferric ion reducing capability (FRAP assay) at 80 °C compared with other drying temperatures. This study indicates that shorter exposure times to hot water and a low drying temperature preserve the antioxidant activities. High antioxidant activities (in addition to its known protein and fat content) suggest that silkworms and silkworm powder can make a valuable contribution to human health.


1993 ◽  
Vol 87 (3) ◽  
pp. 385-391 ◽  
Author(s):  
S. N. Chatterjee ◽  
C. G. P. Rao ◽  
G. K. Chatterjee ◽  
S. K. Ashwath ◽  
A. K. Patnaik

2006 ◽  
Vol 7 (S2) ◽  
pp. 350-356 ◽  
Author(s):  
Hui-peng Yao ◽  
Xiao-feng Wu ◽  
K. Gokulamma

2017 ◽  
Vol 16 ◽  
pp. 27
Author(s):  
K. Sashindran Nair ◽  
Jula S. Nair ◽  
V.A. Vijayan

A juvenoid. R394 (Ethyl 9-cyclohexyl-3,7-dimethyl-2,4-nonadienoate) was applied topically to 5th instar silkworm, Bombyx mori L. larvae (Hybrid: KA x NB4D2) at a dose of 0.039 nl/larva at 24, 48, 72 and 96 h, for silk yield improvement. Three major selected tissues viz., posterior silk gland (PSG), haemolymph and fat body were collected from fully-grown larvae and the total protein, total carbohydrate and total lipid contents were estimated following standard procedures. The result indicated that the content of these primary metabolites varied significantly in the selected tissues depending on the time of juvenoid application. The highest protein content was observed in the haemolymph and silk gland in the larvae treated at 72 h whereas the fat body protein content was lowest for the same treatment. The total carbohydrate was recorded lowest in the 72 h treated larvae as against the highest in the control both in haemolymph and fat body with no significant change in PSG. The total lipid content did not show any notable variation in the concentration on juvenoid administration except in the silk gland treated up to 72 h which showed a decline. The results indicate that the juvenoid induces tissue-specific responses in terms of turnover in primary metabolites which commensurates with the corresponding changes observed in the cocoon weight and cocoon shell weight.


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