Genetic variation in the heat-stress survival of embryos is largely decoupled from adult thermotolerance in an intercontinental set of recombinant lines of Drosophila melanogaster

2021 ◽  
pp. 103119
Author(s):  
Miguel A. Borda ◽  
Federico H. Gomez ◽  
Pablo Sambucetti ◽  
Fabian M. Norry
Keyword(s):  
Drosophila Melanogaster ◽  
Genetic Variation ◽  
Heat Stress ◽  
Stress Survival

Genetic variation for egg-to-adult survival in Drosophila melanogaster in a set of recombinant inbred lines reared under heat stress in a natural thermal environment

2018 ◽  
Vol 166 (10) ◽  
pp. 863-872 ◽  
Author(s):  
Miguel Angel Borda ◽  
Pablo Daniel Sambucetti ◽  
Federico Hernan Gomez ◽  
Fabian Marcelo Norry
Keyword(s):  
Drosophila Melanogaster ◽  
Genetic Variation ◽  
Heat Stress ◽  
Recombinant Inbred ◽  
Thermal Environment ◽  
Recombinant Inbred Lines ◽  
Inbred Lines ◽  
Adult Survival

Heat-stress survival in the pre-adult stage of the life cycle in an intercontinental set of recombinant inbred lines of Drosophila melanogaster

2013 ◽  
Vol 216 (15) ◽  
pp. 2953-2959 ◽  
Author(s):  
P. Sambucetti ◽  
A. C. Scannapieco ◽  
V. Loeschcke ◽  
F. M. Norry
Keyword(s):  
Drosophila Melanogaster ◽  
Heat Stress ◽  
Life Cycle ◽  
Recombinant Inbred ◽  
Adult Stage ◽  
Recombinant Inbred Lines ◽  
Inbred Lines ◽  
Stress Survival

scholarly journals Female Genotypes Affect Sperm Displacement in Drosophila

Genetics ◽  
1998 ◽  
Vol 149 (3) ◽  
pp. 1487-1493 ◽  
Author(s):  
Andrew G Clark ◽  
David J Begun
Keyword(s):  
Drosophila Melanogaster ◽  
Genetic Variation ◽  
Natural Populations ◽  
Isogenic Line ◽  
Female Fitness ◽  
Sperm Displacement ◽  
Extensive Variation ◽  
Polymorphic Genes ◽  
Displacement Parameter ◽  
Competitive Success

Abstract Differential success of sperm is likely to be an important component of fitness. Extensive variation among male genotypes in competitive success of sperm in multiply mated females has been documented for Drosophila melanogaster. However, virtually all previous studies considered the female to be a passive vessel. Nevertheless, under certain conditions female fitness could be determined by her role in mediating use of sperm from multiple males. Here we ask whether females differ among genotypes in their tendency to exhibit last-male precedence. Competition of sperm from two tester male genotypes (bwD and B3-09, a third-chromosome isogenic line from Beltsville, MD) was quantified by doubly mating female lines that had been rendered homozygous for X, second, or third chromosomes isolated from natural populations. The composite sperm displacement parameter, P2′, was highly heterogeneous among lines, whether or not viability effects were compensated, implying the presence of polymorphic genes affecting access of sperm to eggs. Genetic variation of this type is completely neutral in the absence of pleiotropy or interaction between variation in the two sexes.

scholarly journals Genotype and Trait Specific Responses to Rapamycin Intake in Drosophila melanogaster

Insects ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 474
Author(s):  
Palle Duun Rohde ◽  
Asbjørn Bøcker ◽  
Caroline Amalie Bastholm Jensen ◽  
Anne Louise Bergstrøm ◽  
Morten Ib Juul Madsen ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Heat Stress ◽  
Side Effects ◽  
Protein Kinase ◽  
Stress Tolerance ◽  
Treated Group ◽  
Model System ◽  
Treatment Efficiency ◽  
Evolutionarily Conserved ◽  
Heat Stress Tolerance

Rapamycin is a powerful inhibitor of the TOR (Target of Rapamycin) pathway, which is an evolutionarily conserved protein kinase, that plays a central role in plants and animals. Rapamycin is used globally as an immunosuppressant and as an anti-aging medicine. Despite widespread use, treatment efficiency varies considerably across patients, and little is known about potential side effects. Here we seek to investigate the effects of rapamycin by using Drosophila melanogaster as model system. Six isogenic D. melanogaster lines were assessed for their fecundity, male longevity and male heat stress tolerance with or without rapamycin treatment. The results showed increased longevity and heat stress tolerance for male flies treated with rapamycin. Conversely, the fecundity of rapamycin-exposed individuals was lower than for flies from the non-treated group, suggesting unwanted side effects of the drug in D. melanogaster. We found strong evidence for genotype-by-treatment interactions suggesting that a ‘one size fits all’ approach when it comes to treatment with rapamycin is not recommendable. The beneficial responses to rapamycin exposure for stress tolerance and longevity are in agreement with previous findings, however, the unexpected effects on reproduction are worrying and need further investigation and question common believes that rapamycin constitutes a harmless drug.

scholarly journals Genetic variation in the nutrition of Drosophila melanogaster—some general inferences

1962 ◽  
Vol 21 (2) ◽  
pp. 169-178 ◽  
Author(s):  
Forbes W. Robertson
Keyword(s):  
Drosophila Melanogaster ◽  
Genetic Variation

Genetic variation in cytochrome P-450-dependent demethylation in Drosophila melanogaster

1987 ◽  
Vol 36 (14) ◽  
pp. 2279-2282 ◽  
Author(s):  
Inger Hällström
Keyword(s):  
Drosophila Melanogaster ◽  
Genetic Variation ◽  
Cytochrome P 450

scholarly journals Genetic variation in the dietary sucrose modulation of enzyme activities in Drosophila melanogaster

1984 ◽  
Vol 43 (3) ◽  
pp. 307-321 ◽  
Author(s):  
Billy W. Geer ◽  
Cathy C. Laurie-Ahlberg
Keyword(s):  
Drosophila Melanogaster ◽  
Genetic Variation ◽  
Enzyme Activities ◽  
Glycogen Synthesis ◽  
Krebs Cycle ◽  
Natural Populations ◽  
Substitution Lines ◽  
Chromosome Substitution Lines ◽  
High Sucrose Diet ◽  
Selection Of

SUMMARYGenetic variation in the modulating effect of dietary sucrose was assessed in Drosophila melanogaster by examining 27 chromosome substitution lines coisogenic for the X and second chromosomes and possessing different third isogenic chromosomes derived from natural populations. An increase in the concentration of sucrose from 0·1% to 5% in modified Sang's medium C significantly altered the activities of 11 of 15 enzyme activities in third instar larvae, indicating that dietary sucrose modulates many, but not all, of the enzymes of D. melanogaster. A high sucrose diet promoted high activities of enzymes associated with lipid and glycogen synthesis and low activities of enzymes of the glycolytic and Krebs cycle pathways, reflecting the physiological requirements of the animal. Analyses of variance revealed significant genetic variation in the degrees to which sucrose modulated several enzyme activities. Analysis of correlations revealed some relationships between enzymes in the genetic effects on the modulation process. These observations suggest that adaptive evolutionary change may depend in part on the selection of enzyme activity modifiers that are distributed throughout the genome.

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