Species-specific identification of variola, monkeypox, cowpox, and vaccinia viruses by multiplex real-time PCR assay

2011 ◽  
Vol 175 (2) ◽  
pp. 163-169 ◽  
Author(s):  
Sergei N. Shchelkunov ◽  
Dmitrii N. Shcherbakov ◽  
Rinat A. Maksyutov ◽  
Elena V. Gavrilova
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Pcr Assay ◽  
Specific Identification ◽  
Vaccinia Viruses ◽  
Species Specific

scholarly journals Characterization of Plasmodium ovale curtisi and P. ovale wallikeri in Western Kenya Utilizing a Novel Species-specific Real-time PCR Assay

2015 ◽  
Vol 9 (1) ◽  
pp. e0003469 ◽  
Author(s):  
Robin H. Miller ◽  
Clifford O. Obuya ◽  
Elizabeth W. Wanja ◽  
Bernhards Ogutu ◽  
John Waitumbi ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Novel Species ◽  
Plasmodium Ovale ◽  
Pcr Assay ◽  
Western Kenya ◽  
Plasmodium Ovale Curtisi ◽  
Species Specific

scholarly journals Rapid Species Identification of Cooked Poisonous Mushrooms by Using Real-Time PCR

2008 ◽  
Vol 74 (10) ◽  
pp. 3306-3309 ◽  
Author(s):  
Kazuhiko Maeta ◽  
Tomoya Ochi ◽  
Keisuke Tokimoto ◽  
Norihiro Shimomura ◽  
Nitaro Maekawa ◽  
...  
Keyword(s):  
Real Time ◽  
Species Identification ◽  
Real Time Pcr ◽  
Specific Test ◽  
Specific Identification ◽  
Specific Fluorescence ◽  
Species Specific ◽  
Poisonous Mushrooms

ABSTRACT Species-specific identification of the major cooked and fresh poisonous mushrooms in Japan was performed using a real-time PCR system. Specific fluorescence signals were detected, and no nonspecific signals were detected. Therefore, we succeeded in developing a species-specific test for the identification of poisonous mushrooms within 1.5 h.

scholarly journals Detection of Dientamoeba fragilis in animal faeces using species specific real time PCR assay

2016 ◽  
Vol 227 ◽  
pp. 42-47 ◽  
Author(s):  
Douglas Chan ◽  
Joel Barratt ◽  
Tamalee Roberts ◽  
Owen Phillips ◽  
Jan Šlapeta ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Pcr Assay ◽  
Dientamoeba Fragilis ◽  
Species Specific

scholarly journals Development of a species-specific TaqMan-MGB real-time PCR assay to quantify Olsenella scatoligenes in pigs offered a chicory root-based diet

AMB Express ◽  
2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Xiaoqiong Li ◽  
Bent Borg Jensen ◽  
Ole Højberg ◽  
Samantha Joan Noel ◽  
Nuria Canibe
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Pcr Assay ◽  
Chicory Root ◽  
Species Specific

Development of a real-time PCR assay for detection of Mytilus species specific alleles: Application to a sampling survey in Scotland

2008 ◽  
Vol 367 (2) ◽  
pp. 253-258 ◽  
Author(s):  
P.J. Dias ◽  
L. Sollelis ◽  
E.J. Cook ◽  
S.B. Piertney ◽  
I.M. Davies ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Pcr Assay ◽  
Species Specific ◽  
Sampling Survey

Species-specific real-time PCR assay for the detection of Streptococcus suis from clinical specimens

2016 ◽  
Vol 85 (2) ◽  
pp. 131-132 ◽  
Author(s):  
Velusamy Srinivasan ◽  
Lesley McGee ◽  
Berthe-Marie Njanpop-Lafourcade ◽  
Jennifer Moïsi ◽  
Bernard Beall
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Streptococcus Suis ◽  
Pcr Assay ◽  
Clinical Specimens ◽  
Species Specific

scholarly journals A novel, species-specific, real-time PCR assay for the detection of the emerging zoonotic parasite Ancylostoma ceylanicum in human stool

2017 ◽  
Vol 11 (7) ◽  
pp. e0005734 ◽  
Author(s):  
Marina Papaiakovou ◽  
Nils Pilotte ◽  
Jessica R. Grant ◽  
Rebecca J. Traub ◽  
Stacey Llewellyn ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Novel Species ◽  
Pcr Assay ◽  
Ancylostoma Ceylanicum ◽  
Zoonotic Parasite ◽  
Species Specific ◽  
Human Stool

Identification of New PCR Targets and its Validation for Development of Nucleic Acid-based Detection Assay for Melioidosis

2016 ◽  
Vol 1 (1) ◽  
pp. 18
Author(s):  
Sonia Arora ◽  
Duraipandian Thavaselvam ◽  
Archna Prakash ◽  
Ashu Kumar ◽  
Anita Barua ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Bacterial Species ◽  
Cost Effective ◽  
Sybr Green ◽  
Specific Gene ◽  
Pcr Assay ◽  
Gene Target ◽  
Geographical Regions ◽  
Species Specific

Burkholderia pseudomallei the gram negative, soil saprophyte is the causative agent of melioidosis in human and animals. Development of rapid, sensitive, species specific and cost effective molecular assays are needed for detection of B. pseudomallei from clinical and environmental samples and to differentiate it from other closely related bacterial species. In this study, insilico approach was used to identify new species specific gene targets for molecular diagnosis of B. pseudomallei. The identified targets were then analyzed by SYBR Green real time PCR assay for their specificity, sensitivity and presence across different Indian clinical and soil isolates of B. pseudomallei. Out of the three targets studied SYBR Green real time PCR assay targeting bpss0091 gene of B. pseudomallei was found 100% specific, having detection limit of 12.3fg/µl DNA. The bpss0091 gene target was present in all clinical and soil isolates of B. pseudomallei tested thus suggesting bpss0091 gene based SYBR Green real time PCR assay will be useful for detection of B. pseudomallei in different geographical regions.

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