scholarly journals A novel multiplex poliovirus binding inhibition assay applicable for large serosurveillance and vaccine studies, without the use of live poliovirus

2017 ◽  
Vol 241 ◽  
pp. 15-23 ◽  
Author(s):  
Rutger M. Schepp ◽  
Guy A.M. Berbers ◽  
José A. Ferreira ◽  
Johan H. Reimerink ◽  
Fiona R. van der Klis
Keyword(s):  
Inhibition Assay ◽  
Binding Inhibition ◽  
Binding Inhibition Assay

scholarly journals Improved Specificity of a Multiplex Immunoassay for Quantitation of Anti-Diphtheria Toxin Antibodies with the Use of Diphtheria Toxoid

2011 ◽  
Vol 18 (7) ◽  
pp. 1183-1186 ◽  
Author(s):  
Pieter G. M. van Gageldonk ◽  
Christina von Hunolstein ◽  
Fiona R. M. van der Klis ◽  
Guy A. M. Berbers
Keyword(s):  
Diphtheria Toxin ◽  
Neutralization Test ◽  
Diphtheria Toxoid ◽  
Inhibition Assay ◽  
Nonspecific Binding ◽  
Serum Samples ◽  
Multiplex Immunoassay ◽  
Toxin Binding ◽  
Binding Inhibition ◽  
Binding Inhibition Assay

ABSTRACTA nonspecific binding of antibodies to diphtheria toxin, especially in adult serum samples, was observed in our diphtheria-tetanus-pertussis multiplex immunoassay (DTaP4 MIA). This can be significantly reduced by the use of diphtheria toxoid, achieving a good correlation with the Vero cell neutralization test and the toxin binding inhibition assay.

TSH Binding Inhibition Assay: Prediction and Treatment of Neonatal Graves' Disease

1991 ◽  
Vol 4 (4) ◽  
Author(s):  
P.M. Hale ◽  
M. Liebert ◽  
J.C. Sisson ◽  
T. L. Whiteside ◽  
NJ. Hopwood ◽  
...  
Keyword(s):  
Graves Disease ◽  
Inhibition Assay ◽  
Binding Inhibition ◽  
Binding Inhibition Assay

scholarly journals The Fc receptor on thymus-derived lymphocytes. IV. Inhibition of binding of antigen-antibody complexes to Fc receptor-positive T cells by anti-Ia sera.

1977 ◽  
Vol 145 (1) ◽  
pp. 187-203 ◽  
Author(s):  
R D Stout ◽  
D B Murphy ◽  
H O McDevitt ◽  
L A Herzenberg
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Lymphocytes ◽  
Fc Receptor ◽  
Inhibition Assay ◽  
Functional Heterogeneity ◽  
Peripheral T Cells ◽  
Binding Inhibition ◽  
Antigen Antibody ◽  
Binding Inhibition Assay

Treatment of splenic T lymphocytes with anti-Ia antiserum inhibits the binding of antigen-antibody (AgAb) complexes to the majority (less than 50%) of Fc receptor-positive (FcR+) T cells. A similar inhibition was observed with anti-H-2D and anti-H-2K sera but not with anti-Thy 1.2. Despite the presence of Ia determinants on peripheral T cells, as established by the inhibition of AgAb binding, Ia could not be detected on peripheral T cells by immunofluorescence assays. Data obtained with the AgAb-binding inhibition assay indicate that determinants controlled by loci mapping in the I-A and I-C, S, or G regions are present on the FcR+ T cells. Evidence is presented that subpopulations of T cells within the FcR+ T-cell population may be distinguishable on the basis of which I-region-controlled determinant is expressed. The data are discussed in terms of phenotypic and functional heterogeneity of T lymphocytes.

Binding Inhibition Assay Using Fiber-Optic Based Biosensor for the Detection of Foodborne Pathogens

2006 ◽  
Vol 321-323 ◽  
pp. 1145-1150 ◽  
Author(s):  
Mark T. Morgan ◽  
Gi Young Kim ◽  
Daniel Ess ◽  
Aparna Kothapalli ◽  
Byoung Kwon Hahm ◽  
...  
Keyword(s):  
Foodborne Pathogens ◽  
Salmonella Enteritidis ◽  
Pathogen Detection ◽  
Fiber Optic ◽  
Fiber Surface ◽  
Food Samples ◽  
Fluorescent Light ◽  
Inhibition Assay ◽  
Binding Inhibition ◽  
Binding Inhibition Assay

Frequent outbreaks of foodborne illness have been increasing the need for simple, rapid and sensitive methods to detect foodborne pathogens. Conventional methods for pathogen detection and identification are labor-intensive and take days to complete. Some immunological rapid assays are developed, but these assays still require prolonged enrichment steps. Biosensors have shown great potential for the rapid detection of foodborne pathogens. Among the biosensors, fiber-optic methods have much potential because they can be very sensitive and simple to operate. Fiber-optic biosensors typically use a light transmittable, tapered fiber to send excitation laser light to the detection surface and receive emitted fluorescent light. The fluorescent light excited by an evanescent wave generated by the laser is quantitatively related to fluorophor-labeled biomolecules immobilized on the fiber surface. A portable and automated fiber-optic biosensor, RAPTOR (Research International, Monroe, WA), was used to detect Salmonella enteritidis in food samples. A binding inhibition assay based on the biosensor was developed to detect the bacteria in hot dog samples. The biosensor and the binding inhibition assay could detect 104 cfu/ml of bacteria in less than 10 min of assay time.

Thyrotrophin-binding-inhibition assay: comparison of crude and purified membrane preparations

1982 ◽  
Vol 101 (2) ◽  
pp. 199-204
Author(s):  
Angeles A. Garcia ◽  
Anthony N. Hunter ◽  
John A. Wilkins ◽  
Christian von Westarp
Keyword(s):  
Clinical Status ◽  
Thyroid Tissue ◽  
The Other ◽  
Human Thyroid ◽  
Graves Disease ◽  
Thyroid Antibodies ◽  
Inhibition Assay ◽  
Binding Inhibition ◽  
Normal Human ◽  
Binding Inhibition Assay

Abstract. Two different thyroid membrane preparations (TMP), crude and pure, made from a homogenate of normal human thyroid tissue, were used to test purified serum IgG from 36 patients with Graves' disease, and 10 normal control subjects, in the thyrotrophin-binding-inhibition (TBI) assay. All reagents for the assay were identical, and aliquots of each IgG were tested in both TMP simultaneously, under exactly comparable conditions. Blood was drawn while the patients were hyperthyroid (19), euthyroid (13), or hypothyroid (4); 15 of the patients were untreated and 21 were being treated. The frequency of positive TBI was similar in both TMPs and highest among the 131I-treated patients. Comparison of TBI results in both TMP for each IgG sample revealed wide differences, and 47.1% of the Graves' IgGs were TBI positive in one membrane preparation, while negative in the other. There was no correlation of TBI values between the two TMP, or with clinical status, or the presence of standard thyroid antibodies. The findings indicate that the IgGs of Graves' disease, as now tested in the TBI assay, bind heterogeneous to different fractions of the thyroid membranes.

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