Establishing the optimal fetal bovine serum concentration to support replication of cyprinid herpesvirus 3 in CCB and KF-1 cell lines

2020 ◽  
Vol 276 ◽  
pp. 113733
Author(s):  
Hyoung Jun Kim ◽  
Se Ryun Kwon ◽  
Kei Yuasa
Keyword(s):  
Serum Concentration ◽  
Cell Lines ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Fetal Bovine Serum Concentration ◽  
Cyprinid Herpesvirus 3 ◽  
Fetal Bovine

scholarly journals Comparison between EPC and FHM cell lines on IHN virus susceptivility and effect of fetal bovine serum concentration on IHNV-CPE appearance.

1985 ◽  
Vol 20 (1) ◽  
pp. 69-70
Author(s):  
Teiichi NISHIMURA ◽  
Motohiko SANO ◽  
Fuyuki MOTOYAMA ◽  
Yoshikazu KANAI ◽  
Tokuo SANO
Keyword(s):  
Serum Concentration ◽  
Cell Lines ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Fetal Bovine Serum Concentration ◽  
Fetal Bovine

scholarly journals INVESTIGATING COOLING RATE AND FETAL BOVINE SERUM CONCENTRATION ON SURVIVAL AND STEMNESS OF MESENCHYMAL STEM CELLS AFTER CRYOPRESERVATION

2009 ◽  
Vol 12 (9) ◽  
pp. 12-22
Author(s):  
Phuc Van Pham ◽  
Tam Thanh Nguyen ◽  
Nhung Thi Hong Vuong ◽  
Tuyet Thi Bach Duong ◽  
Ngoc Kim Phan
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Serum Concentration ◽  
Cord Blood ◽  
Umbilical Cord Blood ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Fetal Bovine Serum Concentration ◽  
Fetal Bovine ◽  
Different Sources

Mesenchymal stem cells (MSCs) can be derived from many different sources. Umbilical cord blood is a rich source of MSCs. The cryopreservation of MSCs that MSCs are still alive and differentiate into many different kinds of functional cells is very important. The aims of this research are to identify ratio of alive and dead cells as well as stemness of them after thaw. The results showed that the stemness was not affected by cryopreservative protocols or media. All cells being alive after thaw could form colonies and differentiate into adipocytes and osteoblasts. Ratio of alive and dead cells was affected very much by cryopreservative protocols and media.

Fetal Bovine Serum Concentration Affects Δ9 Desaturase Activity of Trypanosoma cruzi

Lipids ◽  
2010 ◽  
Vol 45 (3) ◽  
pp. 275-283 ◽  
Author(s):  
Ana L. Villasuso ◽  
Patricio Romero ◽  
Mariela Woelke ◽  
Patricia Moyano ◽  
Estela Machado ◽  
...  
Keyword(s):  
Serum Concentration ◽  
Trypanosoma Cruzi ◽  
Bovine Serum ◽  
Desaturase Activity ◽  
Fetal Bovine Serum ◽  
Fetal Bovine Serum Concentration ◽  
Δ9 Desaturase ◽  
Fetal Bovine

scholarly journals Various factors affect lipopolysaccharide sensitization in cell cultures

BioTechniques ◽  
2020 ◽  
Vol 69 (2) ◽  
pp. 126-132
Author(s):  
Nan Yang ◽  
Don D Sin ◽  
Delbert R Dorscheid
Keyword(s):  
Cell Lines ◽  
Cell Cultures ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Signal Loss ◽  
The Media ◽  
Fetal Bovine

Commercially available lipopolysaccharide (LPS) is commonly used in research. Although protocols for its use are well established, we experienced a loss of LPS responsiveness in our cell cultures despite no obvious experimental changes. Our cell lines were stimulated with LPS and the media quantified for LPS responsiveness via an IL-8 ELISA. We discovered that the major cause of signal loss was differences in fetal bovine serum (FBS) formulation and concentration. One FBS formulation was notably better at eliciting an IL-8 signal than the second FBS, and 10% FBS in media was better at inducing LPS responsiveness than lower concentrations. We urge researchers to be aware of inherent variations in seemingly commonplace reagents as they may be unexpected sources of inconsistencies.

scholarly journals Evaluation of the major royal jelly proteins as an alternative to fetal bovine serum in culturing human cell lines

2016 ◽  
Vol 17 (6) ◽  
pp. 476-483 ◽  
Author(s):  
Di Chen ◽  
Xiao-xuan Xin ◽  
Hao-cheng Qian ◽  
Zhang-yin Yu ◽  
Li-rong Shen
Keyword(s):  
Cell Lines ◽  
Human Cell ◽  
Bovine Serum ◽  
Royal Jelly ◽  
Fetal Bovine Serum ◽  
Human Cell Lines ◽  
Fetal Bovine
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