scholarly journals Detection of a characteristic melting profile of aSARS-CoV-2 Kappa variant in Italy using the CoV-2 Variants ELITe MGB® Kit

2022 ◽  
pp. 114458
Author(s):  
Margherita Scapaticci ◽  
Andrea Bartolini ◽  
Francesca Vitone ◽  
Vincenzo Cerreta ◽  
Monica Vignoli ◽  
...  
Keyword(s):  
Melting Profile

scholarly journals Direct Profiling of Environmental Microbial Populations by Thermal Dissociation Analysis of Native rRNAs Hybridized to Oligonucleotide Microarrays

2003 ◽  
Vol 69 (4) ◽  
pp. 2377-2382 ◽  
Author(s):  
Said El Fantroussi ◽  
Hidetoshi Urakawa ◽  
Anne E. Bernhard ◽  
John J. Kelly ◽  
Peter A. Noble ◽  
...  
Keyword(s):  
Thermal Dissociation ◽  
Pcr Amplification ◽  
Estuarine Sediments ◽  
Microbial Populations ◽  
Oligonucleotide Microarrays ◽  
Dissociation Curve ◽  
Melting Profile ◽  
Probe Target ◽  
Study Sites

ABSTRACT Oligonucleotide microarrays were used to profile directly extracted rRNA from environmental microbial populations without PCR amplification. In our initial inspection of two distinct estuarine study sites, the hybridization patterns were reproducible and varied between estuarine sediments of differing salinities. The determination of a thermal dissociation curve (i.e., melting profile) for each probe-target duplex provided information on hybridization specificity, which is essential for confirming adequate discrimination between target and nontarget sequences.

Genes Leave Their Characteristic Marks on the Melting Profile of Plasmid DNAs

1995 ◽  
Vol 50 (9-10) ◽  
pp. 652-655 ◽  
Author(s):  
Igor Z. Zubrzycki ◽  
Horst H. Klump
Keyword(s):  
High Resolution ◽  
Resistance Gene ◽  
High Resolution Melting ◽  
Uv Absorbance ◽  
Melting Profile ◽  
Plasmid Dnas ◽  
Parental Plasmid

Abstract High resolution melting profiles of four linearised plasmids (pUC9, pGV403, pHP2, and pBR322) were recorded by means of UV absorbance vs. teperature scaning. The set of transitions obtained for each plasmid are compared to each another and to the transitions obtained for their excised particular antibiotica resistance gene. It can be shown that each gene leaves a charactersitic mark on the melting profile of its parental plasmid

scholarly journals A mantle melting profile across the Basin and Range, SW USA

2002 ◽  
Vol 107 (B1) ◽  
pp. ECV 5-1-ECV 5-21 ◽  
Author(s):  
K. Wang ◽  
T. Plank ◽  
J. D. Walker ◽  
E. I. Smith
Keyword(s):  
Basin And Range ◽  
Mantle Melting ◽  
Melting Profile

The use of PCR melting profile for typing of Pseudomonas syringae isolates from stone fruit trees

2009 ◽  
Vol 126 (4) ◽  
pp. 437-443 ◽  
Author(s):  
Monika Kałużna ◽  
Joanna Puławska ◽  
Piotr Sobiczewski
Keyword(s):  
Pseudomonas Syringae ◽  
Fruit Trees ◽  
Stone Fruit ◽  
Melting Profile

scholarly journals Evaluation of a PCR Melting Profile Technique for Bacterial Strain Differentiation

2006 ◽  
Vol 44 (7) ◽  
pp. 2327-2332 ◽  
Author(s):  
B. Krawczyk ◽  
A. Samet ◽  
J. Leibner ◽  
A. Sledzinska ◽  
J. Kur
Keyword(s):  
Bacterial Strain ◽  
Melting Profile ◽  
Strain Differentiation ◽  
Profile Technique

Changes in The Cytochemical Properties Of Erythrocyte Nuclei Reactivated By Cell Fusion

1969 ◽  
Vol 4 (1) ◽  
pp. 71-87 ◽  
Author(s):  
L. BOLUND ◽  
N. R. RINGERTZ ◽  
H. HARRIS
Keyword(s):  
Hela Cell ◽  
Cell Fusion ◽  
Dry Mass ◽  
Nuclear Chromatin ◽  
Red Cell ◽  
Melting Profile ◽  
Nuclear Composition ◽  
Erythrocyte Nucleus ◽  
Rna And Dna ◽  
Deoxyribonucleoprotein Complex

When the nucleus of a chick erythrocyte is introduced into the cytoplasm of a HeLa cell it resumes the synthesis of RNA and DNA. This reactivation of the red cell nucleus is associated with an increase in volume and with changes in nuclear composition. These changes have now been studied by quantitative cytochemical techniques. During the process of reactivation the dry mass of the erythrocyte nucleus shows a marked increase which takes place largely before the replication of the DNA begins. Within 24 h of cell fusion, some erythrocyte nuclei already contain an increased amount of DNA, and 48 h after fusion many of them contain twice the normal diploid amount, thus indicating that they have replicated their DNA completely. The physical properties of the nuclear deoxyribonucleoprotein complex also change. The ability of the nuclear chromatin to bind acridine orange increases 4- to 5-fold well before the synthesis of DNA begins; and changes in the melting profile of the deoxyribonucleoprotein suggest that its structure is loosened. This view is also supported by the observation that the reactivity of the erythrocyte nuclei to the Feulgen stain is altered during the early stages of reactivation.

scholarly journals Age-related thermal stability and susceptibility to proteolysis of rat bone collagen

1990 ◽  
Vol 272 (3) ◽  
pp. 697-701 ◽  
Author(s):  
C C Danielsen
Keyword(s):  
Thermal Stability ◽  
Bone Matrix ◽  
Sequential Treatment ◽  
Bone Collagen ◽  
Melting Profile ◽  
Shrinkage Temperature ◽  
Age Related ◽  
Molecular Stability ◽  
Femur Diaphysis ◽  
Old Rats

The shrinkage temperature (Ts) and the pepsin-solubilizability of collagen fibrils in bone matrix obtained from decalcified femur diaphysis from 2-, 5-, 15- and 25-month-old rats were found to decrease with age. Digestion with human fibroblast collagenase dissolved less than half of the collagen, whereas sequential treatment by pepsin followed by collagenase resulted in its complete dissolution. This result shows that collagenase and a telopeptide-cleaving enzyme, when acting in an appropriate sequence, have a great potential for the degradation of bone collagen. The ‘melting’ profile of the pepsin-solubilized collagen showed a biphasic transition with transition peak at 35.9 degrees C and 40.8 degrees C. With increasing age an increasing proportion of the collagen ‘melted’ in the transition peak at 35.9 degrees C (pre-transition), and the ‘melting’ temperature (Tm) of the collagen decreased in parallel with Ts in relation to age. Both Ts and Tm decreased by 3 degrees C in the age span investigated. The age-related change in Ts could therefore be accounted for by the decrease in molecular stability. The collagenase-cleavage products of the bone collagen obtained by the sequential treatment with pepsin and collagenase showed only one peak transition (at 35.1 degrees C), and the Tm for the products was independent of age. The results indicate that the pre-transition for the pepsin-solubilized collagen is due to an age-related decrease in thermal stability may have implications for the mechanical strength and turnover of the bone collagen. In contrast with bone collagen, soft-tissue collagen showed neither the age-dependency of thermal stability nor the characteristic biphasic ‘melting’ profile.

scholarly journals PCR melting profile as a tool for outbreak studies of Salmonella enterica in chickens

2015 ◽  
Vol 11 (1) ◽  
Author(s):  
Anna Zaczek ◽  
Arkadiusz Wojtasik ◽  
Radosław Izdebski ◽  
Elzbieta Gorecka ◽  
Ewelina A. Wojcik ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Melting Profile

The Role of DNA Concentration

1969 ◽  
Vol 24 (5) ◽  
pp. 511-514 ◽  
Author(s):  
Samarendra Basu
Keyword(s):  
Thermal Denaturation ◽  
Melting Profile ◽  
Dna Concentration ◽  
Melting Temperatures

Melting temperatures (Tm) of several DNA’s do not vary with the change of DNA concentration. However, at any temperature above Tm, the fractional hyperchromism was always greater at the lower concentration. This result was also evident in the presence of formaldehyde.Reversibility on renaturation decreased with the progress of thermal denaturation along the melting profile. The effect was much enhanced at low DNA concentration and for a DNA of low G—C content.The occurrence of some permanent changes on the dissociated molecules has been suspected to be the only cause for the observed effects.

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