Relation of porcine myogenin gene PCR/RFLP MspI and reproduction traits of the Czech Large White sows

2007 ◽  
Vol 110 (3) ◽  
pp. 288-291 ◽  
Author(s):  
P. Humpolíček ◽  
T. Urban ◽  
Z. Tvrdoň
Keyword(s):  
Large White ◽  
Pcr Rflp ◽  
Reproduction Traits

scholarly journals Influence of <i>ESR1</i>L and <i>FSHB</i> genes on litter size in Czech Large White sows (short communication)

2006 ◽  
Vol 49 (2) ◽  
pp. 152-157
Author(s):  
P. Humpolíček ◽  
T. Urban ◽  
P.` Horák
Keyword(s):  
Short Communication ◽  
Linear Models ◽  
Receptor Gene ◽  
Mixed Linear Models ◽  
Estrogen Receptor Gene ◽  
Large White ◽  
Esr1 Gene ◽  
Pcr Rflp ◽  
Reproduction Traits ◽  
Beta Subunit Gene

Abstract. The effects of the candidate genes for reproduction, the estrogen receptor gene (ESR1) and the follicle-stimulating hormone beta subunit gene (FSHB), on the performance of Large White sows in the three different herds have been examined. A total number of 271 sows (in case of the ESR1 gene) and 171 sows (in case of the FSHB gene) were genotyped with PCR-RFLP method and analysed to determine whether polymorphisms of the genes influence the efficiency of Large White sows. The influence of these genes in the first, the first to the fourth and the second to the fourth litters was investigated. To discover the genes' associations with reproduction traits, mixed linear models were used. The influence of ESR1 and FSHB genes was not very conclusive in the studied populations. The influence of these genes was different depending on the herd, in which the sows were bred or on the set of analyzed litters.

scholarly journals Association and polymorphism study of seven candidate genes with reproductive traits in three pig breeds in Hungary.

2016 ◽  
Vol 63 (2) ◽  
Author(s):  
Ágnes Hunyadi-Bagi ◽  
Péter Balogh ◽  
Krisztina Nagy ◽  
Szilvia Kusza
Keyword(s):  
Growth Rate ◽  
Association Study ◽  
Significant Influence ◽  
Reproductive Traits ◽  
Large White ◽  
Pig Breeds ◽  
Pcr Rflp ◽  
Reproduction Traits ◽  
Hardy Weinberg Equilibrium ◽  
Selection For

Seven genes (BF, EGF, ESR, FSHB, H2AFZ, LEP and PRLP) were studied as candidate gene influencing eleven reproduction traits (interval between litters (IBL), percent of litter (PL), number of litters (NL), number of piglets born dead (NBD), number of piglet born alive (NBA), total number born (TNB), mean of born alive (MBA), mean of born dead (MBD), mean of born total (MBT), mean of piglets at 21 days of age (M21D) and growth rate (GR) in three pig breeds (Hungarian Large White (HLW), Duroc and Pietrain) by PCR-RFLP. Based on the observed vs. expected genotypes frequencies populations across loci were in Hardy-Weinberg equilibrium (P>0.05). In case HLW breed ESR and FSHβ genes were in disequilibrium. Association study suggested that only EGF gene showed significant influence on the trait NBA and TNB. The AA genotype are preferable for sows, associated with higher NBA and TNB. The longest IBL, and the highest NL is associated with AB and AA genotype of EGF gene. IBL is significantly shorter in case of pigs with AB and AA alleles than BB alleles of PRLP genes. Selection for these SNPs could improve the reproductivity in the studied breeds.

scholarly journals Associations between polymorphism of the steroid 21-hydroxylase gene (<i>CYP21</i>) and litter size of Polish Large White × Polish Landrace sows* (short communication)

2006 ◽  
Vol 49 (2) ◽  
pp. 158-164
Author(s):  
J. Ziemak ◽  
W. Grzesiak
Keyword(s):  
Litter Size ◽  
Short Communication ◽  
Reproductive Traits ◽  
Swine Industry ◽  
Large White ◽  
Leukocyte Antigen ◽  
Cyp21 Gene ◽  
Hydroxylase Gene ◽  
Pcr Rflp ◽  
Reproduction Traits

Abstract. research because of the large economic impact these discoveries could have on the swine industry. The steroid 21-hydroxylase (CYP21) gene is located on chromosome 7 in the middle of the swine leukocyte antigen class (SLA) is regarded as a "candidate – gene" reproduction traits. Associations between polymorphism of the steroid 21-hydroxylase gene (CYP21) and litter size of Polish Large White × Polish Landrace sows were analysed. The 21-hydroxylase genotypes of all 286 animals were determined using a PCR-RFLP procedure. The frequencies of genotypes and alleles of CYP21/NciI and CYP21/HaeIII were follows: 0.077 – AA, 0.308 – AB, 0.615 – BB and 0.231 for CYP21/NciIA, 0.769 for CYP21/NciIB; 0.010 – AA, 0.420 – AB, 0.570 – BB and 0.221 for CYP21/HaeIIIA and 0.779 for CYP21/HaeIIIB. The CYP21/NciI genotype was significantly associated with the total number of piglets born, born alive and alive at weaning in the 5th – 10th parities. The sows with BB genotype had significantly (P≤0.01) higher level of traits than the animals with AB genotype. Analysis of reproductive traits in dependence on CYP21/NciI genotypes showed the statistically significant differences (P≤0.05) in number of piglets died before the day of weaned in 2-4 parities. The lowest value of this trait was found for the sows with the AB genotype (2.05%), while the highest – for the pigs with BB genotype (3.54%). Associations between CYP21/HaeIII and reproduction traits were not observed.

scholarly journals The association of leptin gene polymorphism with reproductive usefulness and selected blood biochemical indicators of hybrid sows (Polish Large White × Polish Landrace)

2012 ◽  
Vol 81 (4) ◽  
pp. 333-337
Author(s):  
Agata Wierzchowska ◽  
Anita Kołodziej-Skalska ◽  
Dorota Napierała ◽  
Maria Kawęcka ◽  
Eugenia Jacyno
Keyword(s):  
Reproductive Performance ◽  
Large White ◽  
Blood Biochemical ◽  
Pcr Rflp ◽  
Reproduction Traits ◽  
The Difference ◽  
Biochemical Analyses ◽  
Blood Biochemical Indicators ◽  
Biochemical Indices ◽  
Blood Biochemical Indices

Rapid developments in the field of molecular genetics have led to new possibilities to identify polymorphic genes and to detect quantitative trait loci with major effects on economically important reproduction traits. Our study has defined the influence of polymorphism at position 3469 of the leptin gene on reproductive performance in sows as well as on selected blood biochemical indices in pregnant sows The research involved 173 multiparous sows after the second and third farrowing, crossbreds of Polish Large White and Polish Landrace breeds. The collected data on reproductive performance of sows concerned the number of live births, the number of weaned piglets and the length of weaning-to-estrus interval in the sows. Parities from the first to the fourth were analysed. A sample of blood was collected from each sow in the third month of pregnancy in order to isolate and then analyse DNA with the use of the PCR-RFLP methods. Blood biochemical analyses were performed as well. Two alleles of the leptin-coding gene (T and C) were found in examined herd. In the second parity, where the live birth number was concerned, the difference between the CC (10.4) and TT (9.0) leptin genotypes proved to be significant (P ≤ 0.05). Analyses of blood biochemical indices revealed virtually no significant differences between the genotypes compared. However, it was noted that the glucose concentration was the highest in the sows with TT genotype and the lowest in the sows with CC genotype.

scholarly journals Genetic parameters of production and reproduetive traits in on a farm tested Danish Large White and Landrace swine in Greece

2000 ◽  
Vol 43 (3) ◽  
pp. 287-298
Author(s):  
J. Bizelis ◽  
A. Kominakis ◽  
E. Rogdakis ◽  
F. Georgadopoulou
Keyword(s):  
Maximum Likelihood ◽  
Genetic Correlation ◽  
Genetic Parameters ◽  
Genetic Correlations ◽  
Production Traits ◽  
Likelihood Methods ◽  
Large White ◽  
Reproduction Traits ◽  
Maximum Likelihood Methods ◽  
Genetic And Phenotypic Correlations

Abstract. Production and reproduetive traits in Danish Landrace (LD) and Large White (LW) swine were analysed by restricted maximum likelihood methods to obtain heritabilities as well as genetic and phenotypic correlations. Production traits were: age, backfat thickness (BT), muscle depth (MD) and the ratio BT/MD, adjusted to Standard bodyweight of 85 kg. Reproduction traits were: number of pigs born (NB) and number of pigs weaned (NW) per sow and parity. Heritabilities for age, BT, MD and BT/MD were 0.60, 0.44, 0.51 and 0.42 for LD and 0.36, 0.44, 0.37 and 0.45 for LW, respectively. Genetic correlations between age and BT were −0.22 in LD and – 0.44 in LW. The genetic correlation between age and MD was close to zero in both breeds. Genetic correlation between BT and MD were −0.36 and −0.25 in LD and LW, respectively. Heritabilities for NB were 0.25 in LD and 0.13 in LW while heritabilities for NW were close to zero in both breeds. Genetic correlation between NB and NW was 0.46 and 0.70 in LD and LW, respectively.

scholarly journals Association of non-synonymous SNPs of <i>OPN</i> gene with litter size traits in pigs

2015 ◽  
Vol 58 (2) ◽  
pp. 317-323 ◽  
Author(s):  
T. Kumchoo ◽  
S. Mekchay
Keyword(s):  
Litter Size ◽  
Reproductive Traits ◽  
Snp Markers ◽  
Strong Linkage Disequilibrium ◽  
Nucleotide Polymorphisms ◽  
Large White ◽  
Single Nucleotide ◽  
Pcr Rflp ◽  
Polymerase Chain ◽  
Acid Exchange

Abstract. Osteopontin (OPN) gene is a secreted phosphoprotein which appears to play a key function in the conceptus implantation, placentation and maintenance of pregnancy in pigs. The objectives of this study were to verify the non-synonymous single nucleotide polymorphisms (SNPs) and their association with litter size traits in commercial Thai Large White pigs. A total of 320 Thai Large White sows were genotyped using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Three SNPs at c.425G> A, c.573T> C and c.881C> T revealed amino acid exchange rates of p.110Ala> Thr, p.159Val> Ala and p.262Pro> Ser, respectively, and were then segregated. These three SNPs were significantly associated with total number born (TNB) and number born alive (NBA) traits. No polymorphisms of the two SNP markers (c.278A> G and c.452T> G) were observed in this study. Moreover, the SNPs at c.425G> A and c.573T> C were found to be in strong linkage disequilibrium. The association of OPN with litter size emphasizes the importance of porcine OPN as a candidate gene for reproductive traits in pig breeding.

scholarly journals Analysis of FTO and PLIN2 Polymorphisms in Relation to Carcass and Meat Quality Traits in Pigs

2019 ◽  
Vol 19 (1) ◽  
pp. 71-83 ◽  
Author(s):  
Daniel Polasik ◽  
Eva-Maria Kamionka ◽  
Mirosław Tyra ◽  
Grzegorz Żak ◽  
Arkadiusz Terman
Keyword(s):  
Meat Quality ◽  
Fat Content ◽  
Longissimus Dorsi ◽  
Quality Traits ◽  
Large White ◽  
Meat Quality Traits ◽  
Pcr Rflp ◽  
Perilipin 2 ◽  
Water Holding ◽  
Intramuscular Fat Content

AbstractThe aim of this study was to analyze the association of polymorphisms in alpha-ketoglutarate-dependent dioxygenase (FTO) and perilipin 2 (PLIN2) genes with carcass and meat quality traits in pigs reared in Poland. The research covered 578 sows that belong to the following breeds: Duroc, Hampshire, Polish Landrace, Pietrain, Puławska and Polish Large White. FTO (FM244720:g.400C>G) and PLIN2 (GU461317:g.98G>A) genes variants were determined by means of PCR-RFLP and ACRS-PCR methods respectively. Association between individual genotypes and analyzed traits was calculated by means of GLM procedure for Polish Landrace, Polish Large White and Puławska breeds separately and for all six breeds together in case of FTO gene. The results showed that FTO variants were associated with weight of loin without backfat and skin (WL), loin eye area (AL) and meat percentage (MP) in Polish Large White (P≤0.05), mean backfat thickness from 5 measurements (BFT) and pH measured 45 min after slaughter in m. longissimus dorsi (pH24 ld) as well as with water holding-capacity (WHC) in Puławska breed (P≤0.01). PLIN2 genotypes, however were correlated with WL and height of the loin eye (HL) in Polish Large White and Puławska (P≤0.05), AL in Polish Large White (P≤0.01) as well as luminosity (L*) in Puławska (P≤0.05) pigs. We observed most consistent relationships of PLIN2 SNP with intramuscular fat content (IMF) and WHC. In 3 analyzed breeds GG genotype was connected with highest values of these traits (P≤0.05).

Mapping genes for growth rate and fatness in a Large White x Meishan F2 pig population

1998 ◽  
Vol 1998 ◽  
pp. 7-7 ◽  
Author(s):  
G.A. Walling ◽  
A.L Archibald ◽  
P.M. Visscher ◽  
C.S. Haley
Keyword(s):  
Growth Rate ◽  
Genetic Variation ◽  
Experimental Design ◽  
Statistical Methods ◽  
Population Based ◽  
Genetic Maps ◽  
Large White ◽  
Backcross Population ◽  
Livestock Species ◽  
Reproduction Traits

DNA-based markers and genetic maps of major livestock species have been developed in the last few years. These, together with experimental populations and appropriate statistical methods, provide the tools to dissect causes of economically important genetic variation in livestock. A cross between genetically diverse lines is a powerful experimental design for such a study. In an F2 or backcross population from such a cross, markers are likely to be highly informative and the genes that control the differences between the lines will be segregating. We have been developing such a resource population based on a cross between the British Large White and Chinese Meishan pigs. These breeds differ for many traits, with the Meishan being inferior for growth rate and fatness, but superior for reproduction traits. Here we report the mapping of major genetic effects on growth rate and fatness in our cross.

Genetic and phenotypic relationships between performance test and reproduction traits in pigs selected for components of efficient lean growth rate

1995 ◽  
Vol 1995 ◽  
pp. 9-9
Author(s):  
J. C. Kerr ◽  
N. D. Cameron
Keyword(s):  
Growth Rate ◽  
Performance Test ◽  
Divergent Selection ◽  
Large White ◽  
Selection Strategies ◽  
Lean Growth ◽  
Reproduction Traits ◽  
Breeding Programmes ◽  
Selection For

Genetic and phenotypic relationships between performance test and reproduction traits were estimated in a population of Large White pigs, after five generations of divergent selection for components of efficient lean growth rate. The parameters are required to evaluate alternative selection strategies in pig breeding programmes.

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