An age-dependent association between a leptin C3469T single nucleotide polymorphism and intramuscular fat content in pigs

Abstract. Intramuscular fat content or marbling represents a valuable beef quality trait and is an important determinant of palatability due to its contribution to juiciness and flavour. Meat which has little marbling may be dry and flavourless. Higher levels of intramuscular fat have been associated with increased tenderness, juiciness and flavour of beef (Thompson 2004). Whereas this feature is highly appreciated in some markets (by example Australia, China, Korea, Japan) breeders usually try to increase intramuscular fat deposition in cattle by nutrition. However, genetic factors have been shown to affect marbling in cattle. Thyroglobulin (TG) is a glycoproteic hormone synthesised in the thyroid gland and is the precursor of triiodothyronine (T3) and thyroxine (T4) hormones which influence lipid metabolism. T3 and T4 hormones have been associated with marbling in Wagyu cattle (Mears et al. 2001). The effect of a C/T single nucleotide polymorphism in the 5'-untranslated region of TG gene has been concluded to affect intramuscular fat content in cattle (Barendse 1999). High probability of positive association between the T allele of bovine TG gene and marbling was observed by Wood et al. (2006). Thyroglobulin mainly affects the fat content of longissimus dorsi muscle (LD) (Thaller et al. 2003).

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Association of Twelve Candidate Gene Polymorphisms with the Intramuscular Fat Content and Average Backfat Thickness of Chinese Suhuai Pigs

Animals ◽

10.3390/ani9110858 ◽

2019 ◽

Vol 9 (11) ◽

pp. 858 ◽

Cited By ~ 5

Author(s):

Wang ◽

Li ◽

Zhou ◽

Gao ◽

Liu ◽

...

Keyword(s):

Molecular Markers ◽

Gene Polymorphisms ◽

Genetic Correlations ◽

Intramuscular Fat ◽

Fat Content ◽

Backfat Thickness ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Coefficients Of Variation ◽

Intramuscular Fat Content

The present study aimed to identify the molecular markers for genes that influence intramuscular fat content (IFC), but not average backfat thickness (ABT). A total of 330 Suhuai pigs were slaughtered, and measurements of IFC and ABT were obtained. Phenotypic and genetic correlations between IFC and ABT were calculated. Thirteen single nucleotide polymorphisms (SNPs) among 12 candidate genes for IFC were analyzed, including FABP3, LIPE, IGF1, IGF2, LEP, LEPR, MC4R, PHKG1, RETN, RYR1, SCD, and UBE3C. Associations of the evaluated SNPs with IFCIFC and ABT were performed. Our results showed that the means of IFC and ABT were 1.99 ± 0.03 % and 26.68 ± 0.28 mm, respectively. The coefficients of variation (CVs) of IFC and ABT were 31.21% and 19.36%, respectively. The phenotypic and genetic correlations between IFC and ABT were moderate. Only the FABP3 (rs1110770079) was associated with IFC (p < 0.05) but not with ABT. Besides, there was a tendency for associations of RYR1 (rs344435545) and SCD (rs80912566) with IFC (p < 0.1). Our results indicated that the FABP3 (rs1110770079) SNP could be used as a marker to improve IFC without changing ABT in the Suhuai pig breeding system.

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Effect of single nucleotide polymorphisms on intramuscular fat content in Hungarian Simmental cattle

Asian-Australasian Journal of Animal Sciences ◽

10.5713/ajas.17.0773 ◽

2018 ◽

Vol 31 (9) ◽

pp. 1415-1419 ◽

Cited By ~ 1

Author(s):

István Anton ◽

Balázs Húth ◽

Imre Füller ◽

László Rózsa ◽

Gabriella Holló ◽

...

Keyword(s):

Single Nucleotide Polymorphisms ◽

Intramuscular Fat ◽

Fat Content ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Simmental Cattle ◽

Intramuscular Fat Content

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A splice site single nucleotide polymorphism of thefatty acid binding protein 4gene appears to be associated with intramuscular fat deposition in longissimus muscle in Australian cattle

Animal Genetics ◽

10.1111/j.1365-2052.2009.01913.x ◽

2009 ◽

Vol 40 (5) ◽

pp. 770-773 ◽

Cited By ~ 25

Author(s):

W. Barendse ◽

R. J. Bunch ◽

M. B. Thomas ◽

B. E. Harrison

Keyword(s):

Single Nucleotide Polymorphism ◽

Splice Site ◽

Binding Protein ◽

Intramuscular Fat ◽

Fat Deposition ◽

Longissimus Muscle ◽

Nucleotide Polymorphism ◽

Single Nucleotide ◽

Acid Binding Protein

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Single-nucleotide polymorphisms for matrix metalloprotease-1 can affect perimysial strength and intramuscular fat content but not growth rate of cattle

Animal Production Science ◽

10.1071/an18789 ◽

2020 ◽

Vol 60 (16) ◽

pp. 1869

Author(s):

S. Christensen ◽

C. Monteavaro ◽

P. P. Purslow

Keyword(s):

Growth Rate ◽

Connective Tissue ◽

Growth Curves ◽

Intramuscular Fat ◽

Fat Content ◽

Longissimus Dorsi ◽

Matrix Metalloprotease ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Intramuscular Fat Content

Context Single-nucleotide polymorphisms (SNPs) in the gene coding for matrix metalloprotease-1 (MMP-1) are known to affect the level of intramuscular fat found in cattle. As well as a signalling molecule affecting adipogenesis, MMP-1 is a major collagenase involved in the turnover of connective tissue. Aims The aim of the work was to assess whether SNPs in the gene for MMP-1 may affect the mechanical properties of intramuscular connective tissue, and therefore meat texture. Methods Allelic frequencies of three SNPs for MMP-1 were determined in a group of black Aberdeen Angus cattle whose growth characteristics had been traced for 450 days before slaughter. Associations between the alleles of each of the three SNPs and growth rate, killing out percentage, half-carcass weight, intramuscular fat content, cooking loss, strength of perimysium in cooked M. semitendinosus and Warner–Bratzler peak force of cooked M. longissimus dorsi were studied. Key results None of the SNPs studied had any effect on growth curves, and only one SNP (ss77831914) showed differences in half-carcass weight between alleles. Carcass yield and killing out percentage showed a small difference between alleles of ss7783924. No effects were found on the Warner–Bratzler peak force of M. longissimus dorsi cooked to 70°C. Two SNPs (ss77831914 and ss77831924) showed significant differences between alleles in the raw strength of perimysium in M. semitendinosus and the amount of intramuscular fat. Conclusions Commonly occurring SNPs of the major collagenase MMP-1 can affect the strength of intramuscular connective tissue as well as intramuscular fat content. Although these differences in connective tissue strength do not influence Warner–Bratzler measures of toughness at a cooking temperature of 70°C, they may contribute to differences in toughness in low-temperature, long-time cooking. Implications Because none of the SNPs had effects on the growth curves of the cattle studied, selection of animals with the relevant alleles of SNPs ss77831914 ss77831924 could be used to produce more tender meat without affecting carcass yield.

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