Assessment of physico-chemical, antioxidant and antimicrobial activity of porcine blood protein hydrolysate in pork emulsion stored under aerobic packaging condition at 4 ± 1 °C

LWT ◽  
2018 ◽  
Vol 88 ◽  
pp. 71-79 ◽  
Author(s):  
Akhilesh K. Verma ◽  
Manish Kumar Chatli ◽  
Nitin Mehta ◽  
Pavan Kumar
2018 ◽  
Vol 58 (11) ◽  
pp. 2084 ◽  
Author(s):  
Akhilesh K. Verma ◽  
Manish Kumar Chatli ◽  
Nitin Mehta ◽  
Pavan Kumar

Protein hydrolysates were recovered from porcine blood hydrolysate (PBH) using enzymatic hydrolysis viz. alcalase, trypsin and papain. The PBH were ultra-filtered by using molecular weight cut off, these PBH and different fractions were evaluated for antioxidant and antimicrobial activity. The PBH and fractions were assessed for antioxidant efficacy viz. 2, 2-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid), 2, 2-diphenyl-1-picrylhydrazyl and Ferric reducing antioxidant power assay and antimicrobial activity by zone of inhibition assay. All antioxidant parameters assessed for inhibition activity were observed significantly (P < 0.05) higher for whole PBH whereas among fractions (5–10 kDa), (1–5 kDa) for all hydrolysates had comparatively higher antioxidant efficacy. The results showed that PBH obtained from trypsin and its fractions have highest antioxidant activities. The antimicrobial efficacy was also higher for whole PBH than their respective fractions; however, the zone of inhibition varied significantly (P < 0.05) among different fractions. Results revealed that among enzymes trypsin and alcalase could produce peptides with comparatively higher antimicrobial activity for all tested microbes than papain. The results concluded that porcine blood hydrolysates can be fractioned to get lower molecular weight peptides of interest; however, for addition of whole PBH in meat products or other use could be more useful with regards to its antioxidant, antimicrobial activity and economic production.


Molecules ◽  
2021 ◽  
Vol 26 (21) ◽  
pp. 6678
Author(s):  
Artur Macari ◽  
Rodica Sturza ◽  
Ildiko Lung ◽  
Maria-Loredana Soran ◽  
Ocsana Opriş ◽  
...  

The problem of functional foods with bioactive components of natural origin is current for the food industry. Plant extracts rich in polyphenols with antioxidant and antimicrobial activity are a promising source for use in improving the quality and characteristics of fresh meat and meat products. In this context, the purpose of the present study was to evaluate the physico-chemical, microbiological, sensory properties of sausages prepared with the addition of lyophilized extract of basil, thyme or tarragon. For the beginning, the total amount of polyphenols, the antioxidant and antimicrobial activity of the extracts obtained from three spices were evaluated. In the sausages previously infected with Staphylococcus aureus and Escherichia coli it was observed that there is a much larger number of colonies of microorganisms in the control sample compared to the other samples within 24 and 48 h. Moreover, following the addition of sausage extracts, no changes were found regarding their sensory acceptability.


Author(s):  
Mukhtar Ismail ◽  
Emad M. Abdallah ◽  
Eman R. Elsharkawy

Wild honey is believed to have plentiful nutritional and health benefits. Numerous studies dealing with the characteristics and biological activities of honey have proved its biological activities. However, little is known about Saudi wild honey. In the current study, the physico-chemical properties, antioxidant and antimicrobial activity of five varieties of wild honey collected from different locations in Saudi Arabia were examined. Results showed that, the physico-chemical characteristics of all samples were in compliance with international standards. Qualitative and quantitative analysis of sugar (glucose, fructose and sucrose) was determined by high-performance liquid chromatography (HPLC). The analysis exhibited that fructose is the major sugar type in all examined wild honeys, which was found in highest concentration in flowers honey (46 g/100 g), whereas, the lowest fructose concentration was found in Ziziphus honey (36 g/100 g). All monofloral honey showed significant antioxidant activity. Alfalfa honey recorded the highest antioxidant activity, followed by Ziziphus honey, respectively. However, Tamarisk honey recorded the lowest antioxidant activity among honey samples. Honey's antibacterial activity against five microorganisms displayed differing degrees of inhibition. The overall findings indicated that no antifungal activity was seen against Candida albicans. The antibacterial examination of honey samples revealed non-significant activity against all tested bacteria, except with Staphylococcus aureus ATCC BAA 1026, where the Acacia honey exhibited significant activity against this bacterium (p < 0.05), with a mean of inhibition zone of 12.0+0.0 mm and a minimum inhibitory concentration of 12.5%. The results of the other types of honey were statistically non-significant.


Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
I Kosalec ◽  
M Zovko ◽  
K Sankovic ◽  
D Kremer ◽  
S Pepeljnjak

2019 ◽  
Vol 70 (7) ◽  
pp. 2519-2523
Author(s):  
Denisa Batir Marin ◽  
Oana Cioanca ◽  
Mihai Apostu ◽  
Cristina Gabriela Tuchilus ◽  
Cornelia Mircea ◽  
...  

The objective of the current study is represented by the determination of silica and a phytochemical screening of phenolic derivates of some Equisetum species. The antioxidant and antimicrobial activity for Equisetum pratense Ehrh.,, Equisetum sylvaticum L. and Equisetum telmateia Ehrh. (sin. Equisetum maximum Lam.) were also investigated. The concentration of silicon (Si) in plants was determined by the spectrophotometric method using previous treatment with NaOH 50% both for the stem and the nodal branches [1]. Results obtained varied from 95.12 to 162.10 SiO2 mg/g dry plant which represents 4.44% to 7.58% Si/100g dry sample. Two types of total extracts were obtained using different solvents and were subjected to qualitative and quantitative chemical analysis considering total phenolic content [2]. The highest concentration of investigated compounds was found in the methanolic extract, E. sylvaticum, 196.5mg/g dry sample. Antioxidant activity was monitored spectrophotometrically and expressed in terms of IC50 (�g/mL) [3]. Values gathered ranged from 261.7 to 429.5 �g/mL. The highest capacity to neutralized DPPH radicals was found in E. sylvaticum. In vitro antimicrobial activity was determined using difusimetric method [4]. Testing was performed on four microorganisms: three strains of bacteria and one species of fungi. Different effects were noticed against the bacteria, furthermore the methanol extract appeared to be most efficient. All extracts showed significand antimicrobial activity against Staphylococcus aureus (ATCC 25923) and Candida albicans (ATCC 90028) and weak to no activity against Pseudomonas aeruginosa (ATCC 27853) and Escherichia coli (ATCC 25922).


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