Evaluation of the DNA damage protective activity of the germinated peanut (Arachis hypogaea) in relation to antioxidant and anti-inflammatory activity

LWT ◽  
2019 ◽  
Vol 101 ◽  
pp. 259-268 ◽  
Author(s):  
Apinun Limmongkon ◽  
Jintana Pankam ◽  
Thapakorn Somboon ◽  
Pakwuan Wongshaya ◽  
Phatcharaporn Nopprang
Keyword(s):  
Dna Damage ◽  
Arachis Hypogaea ◽  
Inflammatory Activity ◽  
Protective Activity ◽  
Anti Inflammatory ◽  
Dna Damage Protective

scholarly journals ANTI-OXIDATIVE, ANTI-INFLAMMATORY AND ANTI-ATHEROSCLEROTIC EFFECT OF TAURINE ON HYPERCHOLESTEROLEMIA INDUCED ATHEROSCLEROTIC RATS

2018 ◽  
Vol 10 (3) ◽  
pp. 145
Author(s):  
Harsha Sharma ◽  
Ankita Joshi ◽  
Harsha Lad ◽  
Deepak Bhatnagar
Keyword(s):  
Dna Damage ◽  
Experimental Atherosclerosis ◽  
Protein Carbonyl ◽  
Atherogenic Diet ◽  
Inflammatory Activity ◽  
Glutathione S Transferase ◽  
Protein Carbonyl Content ◽  
Oral Supplementation ◽  
Normal Rat ◽  
Anti Inflammatory

Objective: The present study evaluates the antioxidant, anti-inflammatory and anti-atherosclerotic potency of taurine (2-amino ethane sulfonic acid) when administered orally to hypercholesterolemia induced atherosclerotic rats.Methods: The experimental atherosclerosis was induced by feeding rats with an atherogenic diet comprising of the normal rat chow supplemented with 4 % cholesterol, 1 % cholic acid and 0.5 % thiouracil (CCT diet) for 20 d. Treatment with atorvastatin (10 mg/kg body weight) and taurine (2 % in drinking water) was given to atherosclerotic rats to study antioxidant enzymes (superoxide dismutase, catalase, glutathione-S-transferase), lipid peroxidation in liver, glutathione reductase and protein carbonyl content, extent of DNA damage using the alkaline comet assay, assaying pro-inflammatory cytokines and quantifying atherosclerotic lesions.Results: Oral supplementation of 2 % taurine to hypercholesterolemic rats modulated antioxidant status and significantly reduced malondialdehyde (MDA) content (P<0.05). The extent of DNA damage was also significantly reduced as observed by a reduction in the comet tail index (P<0.05). Taurine exhibited anti-inflammatory activity by significantly inhibiting TNF-α (tumor necrosis factor) and IL-1α (inter leukine) and also inhibited atherosclerotic lesions by clearing lipid deposits on the intimal surface of the rat aorta.Conclusion: Oral administration of taurine to rats showed antioxidant and anti-inflammatory activity by modulating oxidants in favor of reducing oxidative stress and also showed anti-atherosclerotic activity in hypercholesterolemia-induced atherosclerosis.

scholarly journals OXIDATIVE DNA DAMAGE PROTECTIVE AND HEPATOPROTECTIVE ACTIVITY OF TERMI-NALIA BELLERICA (BAHEDA)

2018 ◽  
Vol 10 (1) ◽  
pp. 132 ◽  
Author(s):  
Rasna Gupta ◽  
Ram Lakhan Singh ◽  
Andab Pant
Keyword(s):  
Dna Damage ◽  
Hepg2 Cells ◽  
Oxidative Dna Damage ◽  
Therapeutic Potential ◽  
Hepatoprotective Activity ◽  
Dependent Manner ◽  
Fruit Pulp ◽  
Protective Activity ◽  
Trypan Blue Exclusion ◽  
Dna Damage Protective

Objective: To investigate the ethanolic extracts of Terminalia bellerica for its in vitro DNA damage protective activity against hydroxyl radical (OH•) and hepatoprotective activity against CCl4 induced toxicity in HepG2 liver cells.Methods: The DNA damage assay was performed using supercoiled pBR322 plasmid DNA with Fenton’s reagent. Protection of human liver-derived HepG2 cells against CCl4 induced damage was determined by trypan blue exclusion assay and Tetrazolium bromide salt MTT assay. Liver cells were pre-exposed to 0.5 µmol/ml of CCl4 for 60 min at room temperature and screening assay was carried out in HepG2 cells to evaluate the cytotoxicity of tested extracts with concentration 0.001 to 100 µg/ml.Results: The low dose (0.001-0.1 µg/ml) of leaf and bark has an effect on the percentage viability (101±4.04) and 97±4.04) for leaf and bark extracts respectively) of HepG2 cells. Whereas, the percentage cell viability 99±2.89 and 103±4.04 were found for fruit pulp and seed extracts respectively. The uniform DNA damage protective activity was observed in a dose-dependent manner for leaf, fruit pulp, seed and bark extracts of T. bellerica. Conclusion: Leaf and bark of T. bellerica exhibit 10 fold less toxicity compared to fruit pulp and seed during CCl4 exposure on HepG2 cells suggesting that leaf and bark has more therapeutic potential against hepatotoxicity.

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