excoecaria agallocha
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2022 ◽  
Vol 8 (1) ◽  
Author(s):  
Tamanna Sultana ◽  
Arup Kumar Mitra ◽  
Satadal Das

Abstract Background The incidence of cervical cancer is increasing at an alarming rate in many countries and presently, it is the most common form of malignant cancer being reported among women in India. Development of novel approach for cervical cancer therapy, sparing healthy normal cells overcoming the limitations of prevailing therapies is of prime importance. Mangroves constitute a significant repository of medicinally important plants. Thus, in this study, we aimed to determine the anticancer activity of the mangrove Excoecaria agallocha L. leaf extracts on human cervical cancer (SiHa HPV 16+) cell line with subsequent characterization of the bioactive compounds conferring the anticancer activity and studying the probable underlying mechanism of action of the purified plant extract. Results The plant extract was subjected to silica gel column chromatography and the fractions obtained were analyzed for cytotoxic activity against SiHa cells by MTT assay. One out of the three eluted fractions exhibited selective toxicity against SiHa cells with an IC50 value of 15.538 ± 0.577 µg/mL, while it had no cytotoxic effect on normal healthy human peripheral blood mononuclear cells. High-resolution liquid chromatography mass spectroscopy, coupled to electron spray ionization and diode array detection analysis, led to the structure elucidation and identification of a few pharmacologically important compounds, with Bergenin being present in the highest abundance. Fluorescence microscopy results revealed that the plant extract fraction induced LC3 puncta formation, in EGFP- SiHa cells indicating the onset of autophagy, with simultaneous stimulation of mitophagy. The plant extract also inhibited proliferation of the SiHa-smac-mCherry cells by second mitochondria-derived activator of caspase (SMAC)—induced cytochrome c dependent apoptosis, that was further confirmed with Caspase-3 activation by colorimetric assay. The GFP-dgn in SiHa cells was remarkably protected from proteasomal degradation that might upregulate the survivability of the cells significantly. Flow cytometry followed by Western blot analysis further asserted the ability of the plant extract fraction to cause cell cycle arrest of SiHa cells in the G2/M phase by significantly reducing protein expression levels of cyclin B1 and D1, decreasing Cdc2 level and simultaneously increasing p21 and p53 levels. Conclusion It could be inferred that the aqueous extract of E. agallocha successfully decreased the proliferation of SiHa cervical cancer cells through induction of autophagy and apoptosis in a concerted manner, with simultaneous stimulation of mitophagy and G2/M phase cell cycle arrest, hinting at Bergenin being the major compound conferring the anti-cancer activity of the plant extract. Thus, isolation of the identified bioactive compounds from E. agallocha and their subsequent purification for drug development might serve as a novel medicinal approach for the treatment of cervical cancer in conjugation with existing therapeutic methods.


2022 ◽  
Vol Volume 14 ◽  
pp. 17-26
Author(s):  
Kuzhunellil Raghavanpillai Sabu ◽  
Sujith Sugathan ◽  
Akbar Idhayadhulla ◽  
Melat Woldemariam ◽  
Addis Aklilu ◽  
...  

2021 ◽  
Vol 4 (1) ◽  
pp. 13-18
Author(s):  
Dewy Widiawati ◽  
Engga Marlinsa ◽  
Mardiyati M ◽  
Erik Perdana Putra

This study aims to determine the types of mangrove plants in the Bhadrika tourist park, Bengkulu Province. This study uses the method of observation. The data obtained are then immediately taken to the laboratory for identification. The identification process by observing the roots, stems, leaves, flowers and fruit.  The mangroves found were, Avicennia alba, Lumnitzera racemosa, Excoecaria agallocha, Aegiceras corniculatum, Bruguiera cylindrical. There were 5 types of mangroves in the Bhadrika Tourism Park which belonged to 5 families.


2021 ◽  
Vol 20 (1) ◽  
pp. 176-186
Author(s):  
Faradis Ulyah ◽  
Endah Dwi Hastuti ◽  
Ema Prihastanti

Mangrove merupakan tumbuhan yang berada di wilayah intertidal pesisir laut. Tujuan penelitian ini yaitu menganalisis struktur vegetasi mangrove (frekuensi, kerapatan, dominan) dan karakteristik habitatnya (kualitas lingkungan) di kawasan pesisir pantai kepulauan Karimunjawa. Penelitian dilakukan pada Desember 2019 di 3 stasiun dengan metode plot bertingkat, masing-masing stasiun dibuat 3 transek yang berukuran 10m x 10m (pohon), 5m x 5m (pancang), dan 2m x 2m (semai). Hasil penelitian ditemukan 7 jenis mangrove yaitu Rhizophora stylosa, Avicennia marina, Rhizophora apiculata, Ceriops tagal, Excoecaria agallocha, Lumnitzera racemosa, dan Ceriops decandra. Indeks nilai penting tumbuhan mangrove pada strata pohon, pancang, dan semai paling tinggi adalah Rhizopora stylosa (244,77%), (163,03%), dan (157,96%).  Nilai kerapatan Rhizopora stylosa tingkat pohon, tingkat pancang dan semai yaitu (2.500-10.100 ind/ha), (10.400-48.400 ind/ha), dan (97.500-280.000 ind/ha). Kondisi lingkungan di sekitar kawasan mangrove yaitu rata-rata suhu (28,75%), pasir (10,75%), lanau (51,46%/), lempung (37,79%), salinitas (26,60%), pH (7,26), DO (3,28 mg/L), N total tanah (0,24%), P total tanah (120,49 ppm), C Organik tanah (2,10%), N total air (0,28%), P total air (0,27 mg/L), C Organik air (1,56 mg/L).ABSTRACTMangroves are a plant that are found in the intertidal area of marine coastal environments. The study aim to analyze structure of mangrove vegetation (frequency, density, and dominance) and the mangrove habitat (environmental condition) in Coastal Coast Karimunjawa Island. The research was conducted in December 2019 at the three stations using the stratified plot method, and one stations divided three observation transects sized 10m x 10m (trees), 5m x 5m (saplings), and 2m x 2m (seedlings). The result of the study found seven mangroves species were Rhizophora stylosa, Avicennia marina, Rhizophora apiculata, Ceriops tagal, Excoecaria agallocha, Lumnitzera racemosa, and Ceriops decandra. The highest value index of mangroves for trees, saplings and seedlings is the highest Rhizopora stylosa (244,77%), (163,03%), and (157,96%). Density value Rhizopora stylosa in tree level, saplings, and seedlings were (2.500-10.100 ind/ha), (10.400-48.400 ind/ha), dan (97.500-280.000 ind/ha). The environmental conditions around the mangrove area are average temperature (28,75%), sand (10,75%), silt (51,46%/), clay (37,79%), salinity (26,60%), pH (7,26), Dissolved Oxygen (3,28 mg/L), N total land (0,24%), P total land (120,49 ppm), C Organic land (2,10%), N total water (0,28%), P total water (0,27 mg/L), C Organic water (1,56 mg/L).


2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Md. Abdur Rahman ◽  
Rinku Rani Paul ◽  
Chaina Biswas ◽  
Hakima Akter ◽  
Razina Rouf ◽  
...  

Plants act as a rich source of novel natural pesticides. In the backdrop of the recent revival of interest in developing plant-based insecticides, this study was carried out to investigate the pesticidal activity of Sundarban mangrove plants. A total of nine different plant parts from five plants, namely, Aegiceras corniculatum, Excoecaria agallocha, Heritiera fomes, Xylocarpus moluccensis, and Xylocarpus granatum, were extracted with methanol and tested for insecticidal activity against two common stored product pests Sitophilus oryzae and Sitophilus zeamais using direct contact feeding deterrent wafer disc method. Three bark extracts from A. corniculatum, E. agallocha, and H. fomes showed potent and statistically significant insecticidal activity against both S. oryzae and S. zeamais pests (80–100% mortality). All the active bark extracts were further fractionated using C-18 solid-phase extraction (SPE) columns and tested for their insecticidal activity against S. oryzae pest to identify the active fraction. Only the SPE4 fraction (100% MeOH) from all the three active plants showed the activity against S. oryzae pest with a lethal concentration 50% (LC50) value of 0.5, 1.0, and 1.5 mg/disc for A. corniculatum, E. agallocha, and H. fomes, respectively. The active fraction of A. corniculatum was further profiled for identification of active compounds using LC-ESI-MS and identified (along with some unknown peaks) two previously reported compounds at m/z 625.17630 (isorhamnetin 3-O-rutinoside) and 422.25346 (paspaline) as major constituents. Insecticidal activities of these plants are reported in this study for the first time and would be useful in promoting research aiming for the development of new biopesticides from mangrove plants.


2021 ◽  
pp. 197-203
Author(s):  
N. Jamuna ◽  
R. Sozharajan ◽  
M. Rajaravindran ◽  
S. Natarajan

The effect of NaCl stress on antioxidant enzymes activities was investigated in the leaves of Excoecaria agallocha. Plants were subjected to different levels of NaCl. 100 to 1000 mM. Above 500 mM these mangrove seedlings did not survive. The leaves of 60 day old plants were used for the analysis of enzyme activities. Parameters of enzymatic and non enzymatic antioxidants such as catalase (CAT), peroxidase (POD), ascorbate peroxidase (APX), polyphenol oxidase (PPO), superoxide dismutase (SOD), ascorbic acid (ASA) and alpha tocopherol were determined. The highest CAT, POD, APX, PPO and SOD activities in the leaf and root enhanced gradually up to 300 mM of NaCl, the highest ASA and tocopherol activities in the leaf and root were observed at 500 mM of NaCl. These data suggest that the capacity to limit oxidative damage is important for the salt tolerance of E. agallocha.


2021 ◽  
Vol 45 (1) ◽  
Author(s):  
Tamanna Sultana ◽  
Arup Kumar Mitra ◽  
Satadal Das

Abstract Background The incidence of antibacterial resistance and colon cancer is increasing in India. Antibacterial resistance and chemoresistance demand the need of developing herbal or natural chemotherapeutic agents. Our study thus, aims to determine the antibacterial and anticancer activities of the leaf extracts of the mangrove Excoecaria agallocha. Results Liquid chromatography–mass spectroscopy analysis of the ethanolic E. agallocha extracts revealed the presence of Bergenin. The plant extract fraction containing Bergenin had potent antibacterial action against a resistant strain of Salmonella typhi with an MIC value of 15.7 ± 0.04 µg/mL. Treatment of the bacteria with the plant extract made it moderately susceptible to the antibacterial drugs ampicillin, aztreonam, cefotaxime, chloramphenicol and imipenem. The plant extract caused membrane damage and disrupted the expression of a 33 kDa outer membrane protein (OmpA) in S. typhi. It was plausibly due to this mechanism of the plant extract that made the bacteria susceptible to the antibacterial drugs to a certain extent. Further, fluorescence microscopy analysis revealed the anticancer property of the extract against a human colon cancer (DLD-1) cell line by activation of Caspase-3followed by subsequent apoptosis and exhibited cytotoxicity against the cancerous cell line with an IC50 value of 17.99 ± 1.12 µg/mL. Caspase-3 activity was observed to increase in a dose-dependent manner as determined by spectrophotometric assays. Moreover, the expression of the metalloproteinase-7 (MMP-7) was significantly reduced in plant extract treated DLD-1 colon cancer cells. Conclusion The results indicate that E. agallocha is a novel source of Bergenin, and the plant extract fraction under study may be used in combination therapy along with antibacterial drugs to combat antibacterial resistance of S. typhi and also to alleviate the risks of colon cancers in human. However, further investigations may be undertaken for its therapeutic application and to explore its potential bioactivity against other bacterial strains and human cancer cell lines.


2021 ◽  
Vol 891 (1) ◽  
pp. 012005
Author(s):  
A Nuryawan ◽  
R S Syahputra ◽  
I Azhar ◽  
I Risnasari

Abstract In order to sustain mangrove forests, only branches part of the mangrove trees have been utilized. In this context, these parts have been used as the raw material of wood pellets and briquettes. These solid biofuels are produced by compressing pulverized woody biomass with or without additives in cubic-form or cylindrical units. In this study, five predominant mangrove trees, namely Avicennia marina, Bruguiera sexangula, Excoecaria agallocha, Rhizopora apiculata, and R. mucronata, have been harvested their branches. Wood with and without bark derived from branches has been investigated for their fundamental properties, namely percentage of bark, ash-content, and physical properties (moisture content, density, and specific gravity). These properties will determine the quality class of the resulted wood pellets and briquettes considering pre-treatment or the nature of the branches’ wood.


2021 ◽  
Vol 13 (12) ◽  
pp. 19791-19798
Author(s):  
Sudam Charan Sahu ◽  
Manas Ranjan Mohanta ◽  
N.H. Ravindranath

Heritiera fomes Buch.-Ham. is assessed as an endangered mangrove species by IUCN, and information on population status is lacking. The present study assesses the status of H. fomes in Mahanadi Mangrove Wetland on the east coast of India. Three forest blocks were selected and sampled for this study. Among these, the mean girth at breast height (GBH) of H. fomes was the highest in Hetamundia (HD) forest block. GBH of H. fomes was inversely proportional to the cumulative disturbance index (R2= 0.7244, p value <0.005). The relative density was maximum for H. fomes (56%) at Bhitarkharnasi (BK), and for Excoecaria agallocha at Hetamundia (HD; 35%) & Kansaridia (KD; 54%), respectively. Excoecaria agallocha is a dominant species possibly impacting natural populations of H. fomes. Climate change and rising sea levels may also negatively affect the existence of this species. Therefore, appropriate strategies should be taken for conservation of this globally threatened mangrove species prior to its extinction.


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