Characterization of the growth properties of garlic endophytes and their roles in the formation of black garlic

ABSTRACT Genomic searches were used to reconstruct the putative carotenoid biosynthesis pathway in the pink-pigmented facultative methylotroph Methylobacterium extorquens AM1. Four genes for putative phytoene desaturases were identified. A colorless mutant was obtained by transposon mutagenesis, and the insertion was shown to be in one of the putative phytoene desaturase genes. Mutations in the other three did not affect color. The tetracycline marker was removed from the original transposon mutant, resulting in a pigment-free strain with wild-type growth properties useful as a tool for future experiments.

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The characterization of SV40-transformed cell lines derived from mouse teratocarcinoma: Growth properties and differentiated characteristics

Journal of Cellular Physiology ◽

10.1002/jcp.1040930212 ◽

1977 ◽

Vol 93 (2) ◽

pp. 269-276 ◽

Cited By ~ 19

Author(s):

W. Topp ◽

J. D. Hall ◽

D. Rifkin ◽

A. J. Levine ◽

R. Pollack

Keyword(s):

Cell Lines ◽

Transformed Cell ◽

Growth Properties ◽

Transformed Cell Lines ◽

Mouse Teratocarcinoma

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Satellite-Based Characterization of Convective Growth and Glaciation and Its Relationship to Precipitation Formation over Central Europe

Journal of Applied Meteorology and Climatology ◽

10.1175/jamc-d-16-0293.1 ◽

2017 ◽

Vol 56 (7) ◽

pp. 1827-1845 ◽

Cited By ~ 7

Author(s):

Fabian Senf ◽

Hartwig Deneke

Keyword(s):

Central Europe ◽

Surface Precipitation ◽

Convective Storms ◽

Radar Network ◽

Ice Particles ◽

Growth Properties ◽

Rate Maximum ◽

Precipitation Formation ◽

Distinct Maximum

AbstractThe growth phase of convective storms and their transition to maturity is investigated based on more than 100 cases selected from the years 2012–14 over central Europe. Dynamical growth properties as well as cloud-top glaciation and microphysical characteristics are derived from the SEVIRI imaging radiometer aboard the geostationary Meteosat satellites. In addition, onset and intensity of surface precipitation are related to growth and glaciation processes using observations from the radar network of the German Weather Service. The majority of analyzed cases shows a distinct maximum in cloud-top cooling rate, which is used here for temporal synchronization. Cloud growth spans a period of approximately half an hour. Glaciation rate indicators suggest that freezing 15 min prior to the maximum cooling plays an important role in invigorating convective updrafts through the release of latent heat. Smaller ice particles are found for larger cloud-top cooling, which provides observational evidence that ice particles form later and have less time to grow in stronger convective updrafts. Furthermore, maximum cloud-top height, anvil expansion rate, maximum precipitation intensity, and core size are found to be positively correlated. With respect to the onset of precipitation, this analysis shows a high probability that significant precipitation already occurs 30 min prior to maximum cloud-top cooling.

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Selective epitaxial growth properties and strain characterization of Si1−x Ge x in SiO2 trench arrays

Journal of the Korean Physical Society ◽

10.3938/jkps.70.714 ◽

2017 ◽

Vol 70 (7) ◽

pp. 714-719 ◽

Cited By ~ 4

Author(s):

Sangmo Koo ◽

Hyunchul Jang ◽

Dae-Hong Ko

Keyword(s):

Epitaxial Growth ◽

Strain Characterization ◽

Selective Epitaxial Growth ◽

Growth Properties

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Comparative Virological and Pathogenic Characteristics of Avian Influenza H5N8 Viruses Detected in Wild Birds and Domestic Poultry in Egypt during the Winter of 2016/2017

Viruses ◽

10.3390/v11110990 ◽

2019 ◽

Vol 11 (11) ◽

pp. 990 ◽

Cited By ~ 1

Author(s):

Yassmin Moatasim ◽

Ahmed Kandeil ◽

Basma Emad Aboulhoda ◽

Rabeh El-Shesheny ◽

Maha Alkhazindar ◽

...

Keyword(s):

Avian Influenza ◽

A549 Cells ◽

Wild Birds ◽

Influenza Viruses ◽

Multiple Introductions ◽

Domestic Poultry ◽

Growth Properties ◽

Reassortant Viruses

The surveillance and virological characterization of H5N8 avian influenza viruses are important in order to assess their zoonotic potential. The genetic analyses of the Egyptian H5N8 viruses isolated through active surveillance in wild birds and domestic poultry in the winter of 2016/2017 showed multiple introductions of reassortant viruses. In this study, we investigated and compared the growth kinetics, infectivity, and pathogenicity of the three reassortant forms of H5N8 viruses detected in wild birds and domestic poultry in Egypt during the first introduction wave in the winter of 2016/2017. Three representative H5N8 viruses (abbreviated as 813, 871, and 13666) were selected. The 871/H5N8 virus showed enhanced growth properties in vitro in Madin Darby canine kidney (MDCK) and A549 cells. Interestingly, all viruses replicated well in mice without prior adaptation. Infected C57BL/6 mice showed 20% mortality for 813/H5N8 and 60% mortality for 871/H5N8 and 13666/H5N8, which could be attributed to the genetic differences among the viruses. Studies on the pathogenicity in experimentally infected ducks revealed a range of pathogenic effects, with mortality rate ranging from 0% for 813/H5N8 and 13666/H5N8 to 28% for 871/H5N8. No significant differences were observed among the three compared viruses in infected chickens. Overall, different H5N8 viruses had variable biological characteristics, indicating a continuous need for surveillance and virus characterization efforts.

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Characterization of a cycloheximide-resistant Tetrahymena thermophila mutant which also displays altered growth properties

Molecular and Cellular Biology ◽

10.1128/mcb.3.4.503-510.1983 ◽

1983 ◽

Vol 3 (4) ◽

pp. 503-510

Author(s):

R L Hallberg ◽

E M Hallberg

Keyword(s):

Resistant Strain ◽

Tetrahymena Thermophila ◽

Structural Characteristics ◽

Mutant Gene ◽

Growth Characteristics ◽

Wild Type ◽

Temperature Dependent ◽

Growth Properties ◽

Dependent Growth

A cycloheximide-resistant strain of Tetrahymena thermophila, expressing a mutant chx-B gene (Ares and Bruns, Genetics 90:463-474, 1978), displayed very different temperature-dependent growth characteristics than either wild-type cells or another cycloheximide-resistant strain expressing a different mutant gene. Whereas wild-type cells showed an immediate decline in ribosome translocation rates when shifted from 30 to 38 or 40 degrees C, this mutant strain (X-8) showed no such decline. These results directly correlated with the growth rate differences we found for these cells at these temperatures. By genetic analysis, we showed that the phenotype of cycloheximide resistance cosegregated with the ability to grow rapidly at 40 degrees C. Analyses, both direct and indirect, suggested that a number of functional and structural characteristics of the ribosomes from strain X-8 cells are most likely conformationally different from those of wild-type ribosomes.

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