Antifouling activity of commercial biocides vs. natural and natural-derived products assessed by marine bacteria adhesion bioassay

The defense roles and the antifouling activity of the organic extracts and the major metabolites of the sponges Ircinia oros, I. variabilis and I. spinosula were investigated. The antifeedant activity was tested in experimental aquaria on the generalist predator fish Thalassoma pavo as well as in coastal ecosystems rich in fishes. Some of the major metabolites exhibited high levels of antifeedant activity. The antifouling activity was tested in laboratory assays, against representatives of the major groups of fouling organisms (marine bacteria, marine fungi, diatoms, macroalgae and mussels). All extracts showed promising levels of activity. As was expected, no single extract was active in all tests and some fractions that were effective against one organism showed little or no activity against the others. The high but variable level of antifouling activity in combination with the absence of toxicity (tested on the development of oyster and sea urchin larvae) shows the potential of these metabolites to become ingredients in environmentally friendly antifouling preparations.

Download Full-text

Exploring Antifouling Activity of Biosurfactants Producing Marine Bacteria Isolated from Gulf of California

International Journal of Molecular Sciences ◽

10.3390/ijms21176068 ◽

2020 ◽

Vol 21 (17) ◽

pp. 6068 ◽

Cited By ~ 1

Author(s):

Monserrat Alemán-Vega ◽

Ilse Sánchez-Lozano ◽

Claudia J. Hernández-Guerrero ◽

Claire Hellio ◽

Erika T. Quintana

Keyword(s):

Marine Bacteria ◽

Gulf Of California ◽

Economic Losses ◽

Gram Negative Bacteria ◽

Antifouling Activity ◽

Gram Positive Bacteria ◽

Low Concentrations ◽

Field Assay ◽

Surface Active ◽

Cell Free Culture Supernatant

Biofouling causes major problems and economic losses to marine and shipping industries. In the search for new antifouling agents, marine bacteria with biosurfactants production capability can be an excellent option, due to the amphipathic surface-active characteristic that confers antimicrobial and antibiofilm activities. The aim of this study was to evaluate the antifouling activity of biosurfactants producing marine bacteria from the Gulf of California. The cell free culture supernatant (CFCS) of Bacillus niabensis (S-69), Ralstonia sp. (S-74) (isolated from marine sediment) and of B. niabensis (My-30) (bacteria associated to the sponge Mycale ramulosa) were screened for production of biosurfactants (using hemolysis and drop collapse test, oil displacement and emulsifying activity). The toxicity and antifouling activity were evaluated against biofoulers (bacteria forming biofilm and macrofoulers) both in laboratory and field assays. The results indicate that all bacteria were biosurfactant producers, but the higher capability was shown by B. niabensis (My-30) with high emulsifying properties (E24) of 71%. The CFCS showed moderate toxicity but were considered non-toxic against Artemia franciscana at low concentrations. In the antifouling assay, the CFCS of both strains of B. niabensis showed the best results for the reduction of the biofilm formation (up 50%) against all Gram-positive bacteria and most Gram-negative bacteria with low concentrations. In the field assay, the CFCS of B. niabensis (My-30) led to the reduction of 30% of biofouling compared to the control. The results indicate that the biosurfactant produced by B. niabensis (My-30) has promising antifouling activity.

Download Full-text

Seasonal variation in antifouling activity of crude extracts of the brown alga Bifurcaria bifurcata (Cystoseiraceae) against cyprids of Balanus amphitrite and the marine bacteria Cobetia marina and Pseudoalteromonas haloplanktis

Journal of Experimental Marine Biology and Ecology ◽

10.1016/j.jembe.2004.07.016 ◽

2004 ◽

Vol 313 (1) ◽

pp. 47-62 ◽

Cited By ~ 86

Author(s):

Jean-Philippe Maréchal ◽

Gérald Culioli ◽

Claire Hellio ◽

Hélène Thomas-Guyon ◽

Maureen E. Callow ◽

...

Keyword(s):

Seasonal Variation ◽

Marine Bacteria ◽

Brown Alga ◽

Balanus Amphitrite ◽

Antifouling Activity ◽

Pseudoalteromonas Haloplanktis ◽

Bifurcaria Bifurcata ◽

Crude Extracts ◽

Cobetia Marina

Download Full-text

Study of marine bacteria adhesion on sea-immersed 304 and 316 stainless steels: experimental and theoretical investigations

Journal of Adhesion Science and Technology ◽

10.1080/01694243.2017.1343520 ◽

2017 ◽

Vol 32 (2) ◽

pp. 185-196

Author(s):

Douâae Ou-yahia ◽

Kawtar Fikri-Benbrahim ◽

Hassan Barkai ◽

Moulay Sadiki ◽

Saad Ibnsouda Koraichi

Keyword(s):

Marine Bacteria ◽

Stainless Steels ◽

Theoretical Investigations ◽

Bacteria Adhesion

Download Full-text

Marine Bacteria from Danish Coastal Waters Show Antifouling Activity against the Marine Fouling Bacterium Pseudoalteromonas sp. Strain S91 and Zoospores of the Green Alga Ulva australis Independent of Bacteriocidal Activity

Applied and Environmental Microbiology ◽

10.1128/aem.06038-11 ◽

2011 ◽

Vol 77 (24) ◽

pp. 8557-8567 ◽

Cited By ~ 33

Author(s):

Nete Bernbom ◽

Yoke Yin Ng ◽

Staffan Kjelleberg ◽

Tilmann Harder ◽

Lone Gram

Keyword(s):

Green Alga ◽

Marine Bacteria ◽

Coastal Waters ◽

Sequence Similarity ◽

Rrna Gene ◽

Antifouling Activity ◽

Content Type ◽

Ulva Australis ◽

Pseudoalteromonas Tunicata ◽

Taxonomic Groups

ABSTRACTThe aims of this study were to determine if marine bacteria from Danish coastal waters produce antifouling compounds and if antifouling bacteria could be ascribed to specific niches or seasons. We further assess if antibacterial effect is a good proxy for antifouling activity. We isolated 110 bacteria with anti-Vibrioactivity from different sample types and locations during a 1-year sampling from Danish coastal waters. The strains were identified asPseudoalteromonas,Phaeobacter, andVibrionaceaebased on phenotypic tests and partial 16S rRNA gene sequence similarity. The numbers of bioactive bacteria were significantly higher in warmer than in colder months. While some species were isolated at all sampling locations, others were niche specific. We repeatedly isolatedPhaeobacter gallaeciensisat surfaces from one site andPseudoalteromonas tunicataat two others. Twenty-two strains, representing the major taxonomic groups, different seasons, and isolation strategies, were tested for antiadhesive effect against the marine biofilm-forming bacteriumPseudoalteromonassp. strain S91 and zoospores of the green algaUlva australis. The antiadhesive effects were assessed by quantifying the number of strain S91 orUlvaspores attaching to a preformed biofilm of each of the 22 strains. The strongest antifouling activity was found inPseudoalteromonasstrains. Biofilms ofPseudoalteromonas piscicida,Pseudoalteromonas tunicata, andPseudoalteromonas ulvaepreventedPseudoalteromonasS91 from attaching to steel surfaces.P. piscicidakilled S91 bacteria in the suspension cultures, whereasP. tunicataandP. ulvaedid not; however, they did prevent adhesion by nonbactericidal mechanism(s). SevenPseudoalteromonasspecies, includingP. piscicidaandP. tunicata, reduced the number of settlingUlvazoospores to less than 10% of the number settling on control surfaces. The antifoulingalpPgene was detected only inP. tunicatastrains (with purple and yellow pigmentation), so other compounds/mechanisms must be present in the otherPseudoalteromonasstrains with antifouling activity.

Download Full-text