The free radicals scavenging potential of methanol extract of seeds (Horse gram) of Macrotyloma uniflorum

Abstract Growth habit is a plant architectural trait in grain legumes with no exception of horse gram. Determinacy and indeterminacy are the two types of growth habits reported in horse gram. Relative advantages of the two types of growth habit depend on the production systems to which cultivars are targeted. Dependable information on genetics of growth habit provide clues for adopting the most appropriate selection strategy to breed high yielding horse gram varieties with desired growth habit. Taking cues from the past studies, we hypothesize that growth habit in horse gram is controlled by two genes displaying inhibitory epistasis and indeterminacy is dominant over determinacy. To test this hypothesis, we monitored the inheritance of growth habit in F1, F2 and F3 generations derived from two crosses involving parents differing for growth habit. Contrary to our hypothesis, determinate growth habit of F1s of both the crosses suggested dominance of determinacy over indeterminacy. A good fit of observed segregation of F2 plants to that of the hypothesized segregation in the ratio of 13 determinate: 3 indeterminate plants, besides confirming dominance of determinacy, suggested classical digenic inhibitory epistatic control of growth habit. These results were further confirmed in F3 generation based on goodness of fit between observed numbers of plants segregating for determinacy and indeterminacy and those expected in the ratio of 49 determinate: 15 indeterminate plants. To the best of our knowledge, this is the first report on the inheritance of growth habit in horse gram.

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Limpasu Pericarpium : an Alternative Source of Antioxidant From Borneo with Sequential Maceration Method

Jurnal Profesi Medika Jurnal Kedokteran dan Kesehatan ◽

10.33533/jpm.v15i1.2820 ◽

2021 ◽

Vol 15 (1) ◽

Author(s):

Irfan Zamzani ◽

Nita Triadisti

Keyword(s):

Antioxidant Activity ◽

Free Radicals ◽

Ethyl Acetate ◽

Methanol Extract ◽

Oxidation Process ◽

Antioxidant Activities ◽

Alternative Source ◽

Dpph Method ◽

Indigenous Plant ◽

Ic50 Values

Antioxidants are substances that can slow down the oxidation process of free radicals. Limpasu plant (Baccaurea lanceolata (Miq) Muell. Arg), an indigenous plant of Borneo, is a natural antioxidant source. The purpose of this study was to determine the antioxidant activity of the limpasu pericarpium extract. The extraction of the limpasu pericarpium was done by maceration method using solvents with increasing polarity ranging from n-hexane, ethyl acetate, and methanol. Antioxidant activities of the three extracts were measured by the DPPH and FRAP methods. The IC50 values of n-hexane, ethyl acetate, and methanol extracts, as well as quercetin using the DPPH method were 517,45 µg/mL, 530,64 µg/mL, 10,63 µg/mL and 6,83 µg/mL, respectively. Meanwhile, the IC50 values obtained from FRAP method were 198,96 µg/mL, 190,07 µg/mL, 661,36 µg/mL, and 7,09 µg/mL, respectively. The results revealed that the methanol extract is more potent than other extracts tested for antioxidant activity.

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