Chain formation model is very useful to characterize the magneto-rheological phenomenon and prepare good magneto-rheological fluids. The single-chain model is common to explain the process of chain formation for ferromagnetic particles under magnetic field. With the increment of magnetic field and ferromagnetic particle content, the chain will transit from the single chain to multi-chains. However, there are few literatures involved in this phenomenon. This study investigates the effect of magnetic field and ferromagnetic particles content on the transition. The static yield stresses at different magnetic fields were measured under quasi-static mode for different magneto-rheological fluid samples. The results show that the transition of chain model can be identified on two parameters including the amplitude of static yield angle, [Formula: see text], and the inclined angles distribution, [Formula: see text]. The single-chain model is only effective under low magnetic field and ferromagnetic particles below content of 30% volume. With the increment of magnetic field and ferromagnetic particles content, the transition from single chain to multiple chains will be observed, which validates that there is a transition from the single chain to multi-chains.