Frequency of behavioural activities, growth performance and pork quality were determined for pigs with homozygous-normal (NN) or heterozygous-carrier (Nn) genotypes for porcine stress syndrome receiving dietary supplementation of magnesium aspartate hydrochloride (MAH). One hundred and forty-two pigs, 67.2 ± 1.2 kg, were assigned by genotype and gender to one of three dietary treatments. The pigs were fed a conventional cereal-based pelleted diet (Control), supplemented with a long-term, low-level (LL-MAH; 5 mg kg−1 BW for 43 ± 3 d) or short-term, high-level (SH-MAH; 40 mg kg−1 BW for 7 d) of MAH before slaughter. Supplementation of MAH did not significantly affect the behaviour of pigs before or after transport for slaughter with the exception of SH-MAH-fed pigs showing a higher (P < 0.05) frequency of pre-transport aggression. Pigs with the Nn genotype were, in general, more active 1 h before slaughter showing a higher frequency (P = 0.001) of aggression. Average daily gain of LL-MAH-fed pigs was higher (P < 0.05) than for those pigs fed the Control and SH-MAH diets. Pigs receiving both levels of MAH had lower (P < 0.05) longissimus thoracis (LT) muscle lean depth. In terms of pork quality, there were significant diet-by-genotype interactions. For pigs with the NN genotype, SH-MAH caused higher (P < 0.05) ultimate pH, lower muscle temperature and lower shear value of the LT. For pigs with the Nn genotype, MAH at both levels lowered (P < 0.05) glycogen content and subjective structure score of the LT, but ultimate pH, muscle temperature and lactate content were higher (P < 0.05) than those of Controls. Efficacy of MAH supplementation to pigs appears to be highly dependent on diet and genotype. Key words: Pigs, porcine stress syndrome, magnesium, pork