2D modeling and preliminary in vitro investigation of a prototype high gradient magnetic separator for biomedical applications

Collagenous biomaterials have been used for growing cells in vitro as well as for augmentation and replacement of hard and soft tissues. The substratum used for culturing cells is implicated in the modulation of phenotypic cellular expression, cellular orientation and adhesion. Collagen may have a strong influence on these cellular parameters when used as a substrate in vitro. Clinically, collagen has many applications to wound healing including, skin and bone substitution, tendon, ligament, and nerve replacement. In this report we demonstrate two uses of collagen. First as a fiber to support fibroblast growth in vitro, and second as a demineralized bone/collagen sponge for radial bone defect repair in vivo.For the in vitro study, collagen fibers were prepared as described previously. Primary rat tendon fibroblasts (1° RTF) were isolated and cultured for 5 days on 1 X 15 mm sterile cover slips. Six to seven collagen fibers, were glued parallel to each other onto a circular cover slip (D=18mm) and the 1 X 15mm cover slip populated with 1° RTF was placed at the center perpendicular to the collagen fibers. Fibroblast migration from the 1 x 15mm cover slip onto and along the collagen fibers was measured daily using a phase contrast microscope (Olympus CK-2) with a calibrated eyepiece. Migratory rates for fibroblasts were determined from 36 fibers over 4 days.

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In vitro investigation of Varizella zoster virus as an oncolytic virus in glioblastoma therapy

Aktuelle Neurologie ◽

10.1055/s-0028-1086549 ◽

2008 ◽

Vol 35 (S 01) ◽

Author(s):

H Leske ◽

A Baiker ◽

C Schichor ◽

J.C Tonn ◽

R Goldbrunner ◽

...

Keyword(s):

Oncolytic Virus ◽

In Vitro Investigation ◽

Varizella Zoster Virus

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Cefpodoxime with Antacid, Metal Complexation and Investigation of Antimicrobial Activity, In-Vitro Investigation

American Journal of Biomedical Science & Research ◽

10.34297/ajbsr.2020.08.001318 ◽

2020 ◽

Vol 8 (5) ◽

pp. 452-456

Author(s):

Md Shahidul Islam

Keyword(s):

Antimicrobial Activity ◽

Metal Complexation ◽

In Vitro Investigation

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Evaluation of Antibacterial and Antidiabetic Potential of Silver Nanoparticles using Eugenia Uniflora L. Seed Extract

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2020.13.6.6 ◽

2020 ◽

Vol 13 (6) ◽

pp. 5217-5225

Author(s):

Guru Kumar Dugganaboyana ◽

Chethankumar Mukunda ◽

Suresh Darshini Inakanally

Keyword(s):

Biomedical Applications ◽

Pathogenic Bacteria ◽

Seed Extract ◽

Drug Formulation ◽

Visible Spectroscopy ◽

Plant Materials ◽

X Ray ◽

Eugenia Uniflora ◽

Uv Visible

In recent years, green nanotechnology-based approaches using plant materials have been accepted as an environmentally friendly and cost-effective approach with various biomedical applications. In the current study, AgNPs were synthesized using the seed extract of the Eugenia uniflora L. (E.uniflora). Characterization was done using UV-Visible spectroscopy, X-ray diffraction (XRD), scanning electronic microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX) analyses. The formation of AgNPs has confirmed through UV-Visible spectroscopy (at 466 nm) by the change of color owing to surface Plasmon resonance. Based on the XRD pattern, the crystalline property of AgNPs was established. The functional group existing in seed of E.uniflora extract accountable for the reduction of Ag+ ion and the stabilization of AgNPs was investigated. The morphological structures and elemental composition was determined by SEM and EDX analysis. With the growing application of AgNPs in biomedical perspectives, the biosynthesized AgNPs were evaluated for their antibacterial and along with their antidiabetic potential. The results showed that AgNPs are extremely effective with potent antidiabetic potential at a very low concentration. It also exhibited potential antibacterial activity against the three tested human pathogenic bacteria. Overall, the results highlight the effectiveness and potential applications of AgNPs in biomedical fields such as in the treatment of acute illnesses as well as in drug formulation for treating various diseases such as cancer and diabetes. It could be concluded that E. uniflora seed extract AgNPs can be used efficiently for in vitro evaluation of their antibacterial and antidiabetic effects with potent biomedical applications.

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Conjugates of Classical DNA/RNA Binder with Nucleobase: Chemical, Biochemical and Biomedical Applications

Current Medicinal Chemistry ◽

10.2174/0929867325666180508090640 ◽

2019 ◽

Vol 26 (30) ◽

pp. 5609-5624

Author(s):

Dijana Saftić ◽

Željka Ban ◽

Josipa Matić ◽

Lidija-Marija Tumirv ◽

Ivo Piantanida

Keyword(s):

Molecular Weight ◽

Small Molecules ◽

Biomedical Applications ◽

Protein Targets ◽

New Class ◽

Rna Targets ◽

Covalently Linked ◽

Structural Aspects

: Among the most intensively studied classes of small molecules (molecular weight < 650) in biomedical research are small molecules that non-covalently bind to DNA/RNA, and another intensively studied class is nucleobase derivatives. Both classes have been intensively elaborated in many books and reviews. However, conjugates consisting of DNA/RNA binder covalently linked to nucleobase are much less studied and have not been reviewed in the last two decades. Therefore, this review summarized reports on the design of classical DNA/RNA binder – nucleobase conjugates, as well as data about their interactions with various DNA or RNA targets, and even in some cases protein targets are involved. According to these data, the most important structural aspects of selective or even specific recognition between small molecule and target are proposed, and where possible related biochemical and biomedical aspects were discussed. The general conclusion is that this, rather new class of molecules showed an amazing set of recognition tools for numerous DNA or RNA targets in the last two decades, as well as few intriguing in vitro and in vivo selectivities. Several lead research lines show promising advancements toward either novel, highly selective markers or bioactive, potentially druggable molecules.

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Synthesis of mussel-inspired polydopamine-gallium nanoparticles for biomedical applications

Nanomedicine ◽

10.2217/nnm-2020-0312 ◽

2021 ◽

Author(s):

Jean Valdir Uchôa Teixeira ◽

Fátima Raquel Azevedo Maia ◽

Mariana Carvalho ◽

Rui Reis ◽

Joaquim Miguel Oliveira ◽

...

Keyword(s):

Cell Proliferation ◽

Biomedical Applications ◽

Adipose Derived Stem Cells ◽

Cell Analysis ◽

Shell Formation ◽

X Ray ◽

Alkali Medium ◽

Reproducible Method ◽

Gallium Nanoparticles

Aim: To established a simple, controlled and reproducible method to synthesize gallium (Ga)-coated polydopamine (PDA) nanoparticles (NPs). Materials & methods: PDA NPs were synthesized in alkali medium with posterior Ga shell formation due to ion chelation on the NP surface. Results: The obtained results with energy-dispersive x-ray spectroscopy confirmed the incorporation of Ga on the PDA NP surface. The cytotoxicity of Ga-coated PDA NPs was evaluated in vitro at different concentrations in contact with human adipose-derived stem cells. Further cell analysis also demonstrated the benefit of Ga-coated PDA NPs, which increased the cell proliferation rate compared with noncoated PDA NPs. Conclusion: This study indicated that Ga could work as an appropriate shell for PDA NPs, inducing cell proliferation at the analyzed concentrations.

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An In Vitro Investigation of Synergy or Antagonism between Antimicrobial Combinations against Isolates from Bacterial Keratitis

Investigative Opthalmology & Visual Science ◽

10.1167/iovs.09-4839 ◽

2010 ◽

Vol 51 (8) ◽

pp. 4151 ◽

Cited By ~ 26

Author(s):

Henri Sueke ◽

Stephen B. Kaye ◽

Timothy Neal ◽

Amanda Hall ◽

Stephen Tuft ◽

...

Keyword(s):

Bacterial Keratitis ◽

In Vitro Investigation ◽

Antimicrobial Combinations

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Hexapod Assassins’ Potion: Venom Composition and Bioactivity from the Eurasian Assassin Bug Rhynocoris iracundus

Biomedicines ◽

10.3390/biomedicines9070819 ◽

2021 ◽

Vol 9 (7) ◽

pp. 819

Author(s):

Nicolai Rügen ◽

Timothy P. Jenkins ◽

Natalie Wielsch ◽

Heiko Vogel ◽

Benjamin-Florian Hempel ◽

...

Keyword(s):

Biomedical Applications ◽

Galleria Mellonella ◽

Systemic Reactions ◽

Venom Composition ◽

Assassin Bug ◽

Molecular Components ◽

First Time ◽

Tissue Digestion

Assassin bug venoms are potent and exert diverse biological functions, making them potential biomedical goldmines. Besides feeding functions on arthropods, assassin bugs also use their venom for defense purposes causing localized and systemic reactions in vertebrates. However, assassin bug venoms remain poorly characterized. We collected the venom from the assassin bug Rhynocoris iracundus and investigated its composition and bioactivity in vitro and in vivo. It caused lysis of murine neuroblastoma, hepatoma cells, and healthy murine myoblasts. We demonstrated, for the first time, that assassin bug venom induces neurolysis and suggest that it counteracts paralysis locally via the destruction of neural networks, contributing to tissue digestion. Furthermore, the venom caused paralysis and melanization of Galleria mellonella larvae and pupae, whilst also possessing specific antibacterial activity against Escherichia coli, but not Listeria grayi and Pseudomonas aeruginosa. A combinatorial proteo-transcriptomic approach was performed to identify potential toxins responsible for the observed effects. We identified neurotoxic Ptu1, an inhibitory cystin knot (ICK) toxin homologous to ω-conotoxins from cone snails, cytolytic redulysins homologous to trialysins from hematophagous kissing bugs, and pore-forming hemolysins. Additionally, chitinases and kininogens were found and may be responsible for insecticidal and cytolytic activities. We demonstrate the multifunctionality and complexity of assassin bug venom, which renders its molecular components interesting for potential biomedical applications.

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