Genomic analysis of group A rotavirus G12P[8] including a new Japanese strain revealed evidence for intergenotypic recombination in VP7 and VP4 genes

Group A rotavirus (RVA) strain K8 (RVA/Human-tc/JPN/K8/1977/G1P[9]) was found to have Wa-like VP7 and NSP1 genes and AU-1-like VP4 and NSP5 genes. To determine the exact origin and overall genetic makeup of this unusual RVA strain, the remaining genes (VP1–VP3, VP6 and NSP2–NSP4) of K8 were analysed in this study. Strain K8 exhibited a G1-P[9]-I1-R3-C3-M3-A1-N1-T3-E3-H3 genotype constellation, not reported previously. The VP6 and NSP2 genes of strain K8 were related closely to those of common human Wa-like G1P[8] and/or G3P[8] strains, whilst its VP1–VP3, NSP3 and NSP4 genes were related more closely to those of AU-1-like RVAs and/or AU-1-like genes of multi-reassortant strains than to those of other RVAs. Therefore, strain K8 might have originated from intergenogroup-reassortment events involving acquisition of four Wa-like genes, possibly from G1P[8] RVAs, by an AU-1-like P[9] strain. Whole-genomic analysis of strain K8 has provided important insights into the complex genetic diversity of RVAs.

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Whole genomic analysis of bovine group A rotavirus strains A5-10 and A5-13 provides evidence for close evolutionary relationship with human rotaviruses

Veterinary Microbiology ◽

10.1016/j.vetmic.2016.09.003 ◽

2016 ◽

Vol 195 ◽

pp. 37-57 ◽

Cited By ~ 7

Author(s):

Satoshi Komoto ◽

Yaowapa Pongsuwanna ◽

Ratana Tacharoenmuang ◽

Ratigorn Guntapong ◽

Tomihiko Ide ◽

...

Keyword(s):

Evolutionary Relationship ◽

Genomic Analysis ◽

Group A Rotavirus ◽

Group A

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Genotyping of G/P no typable group A rotavirus strains revealed G2 and G9 genotype circulations in Moroccan children fully vaccinated with Rotarix™

10.26226/morressier.56d6be81d462b80296c95e88 ◽

2016 ◽

Author(s):

Nadia Touil

Keyword(s):

Group A Rotavirus ◽

Moroccan Children ◽

Group A

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Molecular Detection of Group A Rotavirus in under Five Years Old Children with Acute Diarrhoea Admitted to Yangon Children’s Hospital

Myanmar Health Sciences Research Journal ◽

10.34299/mhsrj.00927 ◽

2019 ◽

Vol 31 (1) ◽

pp. 87-92

Keyword(s):

Acute Diarrhoea ◽

Enzyme Linked Immunosorbent Assay ◽

Viral Gastroenteritis ◽

Cross Sectional ◽

Under Five ◽

Group A Rotavirus ◽

Rotavirus Vaccination ◽

Group A ◽

Stool Samples ◽

Considerable Morbidity

Rotaviruses are regarded as the most common cause of viral gastroenteritis and are responsible for considerable morbidity and mortality among children especially under five years of age worldwide. In developing countries like Myanmar, where diarrhoea is in the priority childhood disease, rotavirus surveillance and detection of rotavirus genotypes are utmost important. A hospital-based, cross-sectional descriptive study was conducted at Yangon Children‟s Hospital among under five children admitted for acute diarrhoea from January to October 2016. This study includes detection of Group A rotavirus antigen by commercial enzyme-linked immunosorbent assay (ELISA) and genotyping by multiplex RT-PCR. From a total of 488 collected samples, rotavirus antigen was detected in 219 samples (45%). Rotavirus diarrhoea was most common among the age of 6-11 months (38.8%) followed by 12-23 months (37.9%). The results showed that boys were more commonly affected than girls. Detection of rotavirus positivity was peak in February (57.6 %). Out of 219 stool samples with positive ELISA result, 40 stool samples with high optical density value were proceeded for further determination of G and P genotypes. Regarding distribution of G genotypes, the most common G genotype was G9 which comprised 45%, and that of P genotype was P[8] which comprised 92.5%. Regarding combination of G and P genotypes, the most frequent combination is G9P[8], and it constituted 42.5%. Untypable genotypes were seen in 30% of G and 2.5% of P typing. As rotavirus infection can be prevented by vaccine, WHO recommended that rotavirus vaccination should be included in national immunization program especially in countries where prevalence of rotavirus is high. The distribution of G and P genotypes is important in consideration of appropriate vaccine in pre-vaccination and evaluation of effectiveness of vaccine in post-vaccination period. Therefore, the information on currently circulating genotypes of rotavirus in this study will serve as valuable data for vaccination programme.

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ROTADIAL: the first Nanobody-based immunoassay to detect Group A Rotavirus

Journal of Virological Methods ◽

10.1016/j.jviromet.2021.114279 ◽

2021 ◽

pp. 114279

Author(s):

C.G. Vega ◽

L.L. Garaicoechea ◽

J.I. Degiuseppe ◽

M. Bok ◽

A.A. Rivolta ◽

...

Keyword(s):

Group A Rotavirus ◽

Group A

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Prevalence and Genetic Diversity of Group A Rotavirus Genotypes in Moscow (2019–2020)

Pathogens ◽

10.3390/pathogens10060674 ◽

2021 ◽

Vol 10 (6) ◽

pp. 674

Author(s):

Anton Yuzhakov ◽

Ksenia Yuzhakova ◽

Nadezhda Kulikova ◽

Lidia Kisteneva ◽

Stanislav Cherepushkin ◽

...

Keyword(s):

Infectious Disease ◽

Genetic Diversity ◽

Acute Gastroenteritis ◽

Rt Pcr ◽

Clinical Hospital ◽

Group A Rotavirus ◽

Group A ◽

First Time ◽

Common Combination ◽

Children Under 5

Group A rotavirus (RVA) infection is the leading cause of hospitalization of children under 5 years old, presenting with symptoms of acute gastroenteritis. The aim of our study was to explore the genetic diversity of RVA among patients admitted to Moscow Infectious Disease Clinical Hospital No. 1 with symptoms of acute gastroenteritis. A total of 653 samples were collected from May 2019 through March 2020. Out of them, 135 (20.67%) fecal samples were found to be positive for rotavirus antigen by ELISA. RT-PCR detected rotavirus RNA in 80 samples. Seven G-genotypes (G1, G2, G3, G4, G8, G9, and G12) and three P-genotypes (P[8], P[4], and P[6]) formed 9 different combinations. The most common combination was G9P[8]. However, for the first time in Moscow, the combination G3P[8] took second place. Moreover, all detected viruses of this combination belonged to Equine-like G3P[8] viruses that had never been detected in Russia before. The genotype G8P[8] and G9P[4] rotaviruses were also detected in Moscow for the first time. Among the studied rotaviruses, there were equal proportions of Wa and DS-1-like strains; previous studies showed that Wa-like strains accounted for the largest proportion of rotaviruses in Russia.

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