Developments of bacterial and phytoplankton biomass were assessed during an annual sampling cycle in a eutrophic lake (Lake Aydat, France). Bacterial abundance, estimated by epifluorescence microscopy counting, varied from 0.61 to 12.72 × 106 cells/mL, confirming the eutrophic status attributed to this lake. Cellular densities were nearly homogeneously distributed in the water column. Mean values for bacterial abundance, obtained from three sampling depths (2, 7, and 14 m) were 3.70, 4.23, and 3.66 × 106 cells/mL. In the epilimnion, phytoplankton productivity and community composition, as well as temperature, appeared to control bacterial development. Bacterial biomass varied from 0.5 to 43.5 × 10−2 mg C/L. Mean values for the three sampling depths were 8.1, 10.1, and 9.1 × 10−2 mg C/L. Although bacterial biomass was significantly correlated with bacterial abundance, only 45 to 65% of its variation could be explained by abundance. Individual cell biovolumes varied up to sixfold, from 0.014 μm3 in spring and autumn to 0.090 μm3 in summer. The largest bacterial cells and the most elevated bacterial biomass were reached when bacteria were attached to phytoplankton or detrital organic matter, primarily during summer. Mucilaginous phytoplankton, such as Gomphosphaeria and Sphaerocystis, was found to be particularly good substrate available for the development of large and attached bacteria.Key words: eutrophic lake, bacterioplankton, phytoplankton, seasonal coupling, bacterial attachment.