Protective role of apple cider vinegar (APCV) in CCl4-induced renal damage in wistar rats

Benign prostatic hyperplasia (BPH) is an important key health concern for aging men. Polyphenolic compounds have been found to possess important roles in the inhibition of numerous ailments that involve reactive oxygen species and inflammation. Diosmin is a citrus flavone that possesses antioxidant, anti-inflammatory, antiproliferative, and anticancer activities, so based on these properties of diosmin, we decided to evaluate its effect on testosterone propionate (TP)-induced BPH. A total of 30 Wistar rats were randomly assigned to five groups having six animals in each. This study was of 28 days in which TP (5 mg kg−1) was administered to induce BPH in the last 10 days of the study. It was found that diosmin at the doses of 20 and 40 mg kg−1 significantly reduced malondialdehyde and xanthine oxidase formation in a dose-dependent manner; however, it replenished catalase, glutathione (GSH), and GSH-dependent enzymes, that is, glutathione peroxidase, glutathione reductase, and glutathione- S-transferase significantly against TP-induced BPH. Further, immunohistochemical study showed that diosmin alleviated inflammatory markers (nuclear factor kappa-light-chain-enhancer of activated B cells, cyclooxygenase-2, and interleukin-6). It was also found that diosmin downregulated the expression of androgen receptor and decreased the prostate-specific antigen concentration dose-dependently, significantly against TP-induced BPH. Diosmin also restored histoarchitecture of the prostate in a dose-dependent manner. Findings from the present study revealed the protective role of diosmin against TP-induced BPH in Wistar rats.

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Sprr2f protects against renal injury by decreasing the level of reactive oxygen species in female mice

AJP Renal Physiology ◽

10.1152/ajprenal.00318.2020 ◽

2020 ◽

Vol 319 (5) ◽

pp. F876-F884

Author(s):

Kieu My Huynh ◽

Anny Chuu-Yun Wong ◽

Bo Wu ◽

Marc Horschman ◽

Hongjuan Zhao ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Renal Injury ◽

Renal Damage ◽

Reactive Oxygen ◽

Protective Role ◽

Oxygen Species ◽

Rich Region ◽

Female Mice ◽

Proline Rich Region

Renal injury leads to chronic kidney disease, with which women are not only more likely to be diagnosed than men but have poorer outcomes as well. We have previously shown that expression of small proline-rich region 2f ( Sprr2f), a member of the small proline-rich region ( Sprr) gene family, is increased several hundredfold after renal injury using a unilateral ureteral obstruction (UUO) mouse model. To better understand the role of Sprr2f in renal injury, we generated a Sprr2f knockout ( Sprr2f-KO) mouse model using CRISPR-Cas9 technology. Sprr2f-KO female mice showed greater renal damage after UUO compared with wild-type ( Sprr2f-WT) animals, as evidenced by higher hydroxyproline levels and denser collagen staining, indicating a protective role of Sprr2f during renal injury. Gene expression profiling by RNA sequencing identified 162 genes whose expression levels were significantly different between day 0 and day 5 after UUO in Sprr2f-KO mice. Of the 162 genes, 121 genes were upregulated after UUO and enriched with those involved in oxidation-reduction, a phenomenon not observed in Sprr2f-WT animals, suggesting a protective role of Sprr2f in UUO through defense against oxidative damage. Consistently, bilateral ischemia-reperfusion injury resulted in higher serum blood urea nitrogen levels and higher tissue reactive oxygen species in Sprr2f-KO compared with Sprr2f-WT female mice. Moreover, cultured renal epithelial cells from Sprr2f-KO female mice showed lower viability after oxidative damage induced by menadione compared with Sprr2f-WT cells that could be rescued by supplementation with reduced glutathione, suggesting that Sprr2f induction after renal damage acts as a defense against reactive oxygen species.

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Protective Role of Green Synthesized Gold Nanoparticles Using Terminalia arjuna against Acetaminophen Induced Hematological Alterations in Male Wistar Rats

10.35248/2157-7439.19.10.530 ◽

2019 ◽

Vol 10 (2) ◽

Cited By ~ 6

Author(s):

Mousumi Mitra ◽

Amit Bandyopadhyay ◽

Gouriprasad Datta ◽

Dilip K Nandi

Keyword(s):

Gold Nanoparticles ◽

Wistar Rats ◽

Protective Role ◽

Terminalia Arjuna ◽

Male Wistar Rats ◽

Hematological Alterations

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FGF21 prevents low protein diet-induced renal inflammation in aged mice

AJP Renal Physiology ◽

10.1152/ajprenal.00107.2021 ◽

2021 ◽

Author(s):

Han Fang ◽

Sujoy Ghosh ◽

Landon Sims ◽

Kirsten P. Stone ◽

Cristal M Hill ◽

...

Keyword(s):

Transcriptional Activation ◽

Renal Damage ◽

Protective Role ◽

Wild Type ◽

Kidney Weight ◽

Albumin Creatinine Ratio ◽

Low Protein ◽

The Impact ◽

Protein Diets

Low protein diets extend lifespan through a comprehensive improvement in metabolic health across multiple tissues and organs. Many of these metabolic responses to protein restriction are secondary to transcriptional activation and release of FGF21 from the liver. However, the effects of a low protein (LP) diet on the kidney in the context of aging has not been examined. Therefore, the goal of the current study was to investigate the impact of chronic consumption of a LP diet on the kidney in aging mice lacking FGF21. Wild type (WT, C57BL/6J) and FGF21 KO mice were fed a normal protein (NP, 20% casein) or a LP (5% casein) diet ad libitum from 3 to19 months of age. The LP diet led to a decrease in kidney weight and urinary albumin/creatinine ratio in both WT and FGF21 KO mice. Although the LP diet produced only mild fibrosis and infiltration of leukocytes in WT kidneys, the effects were significantly exacerbated by the absence of FGF21. Accordingly, transcriptomic analysis showed that inflammation-related pathways were significantly enriched and upregulated in response to LP diet in FGF21 KO but not WT mice. Collectively, these data demonstrate that the LP diet negatively affected the kidney during aging, but in the absence of FGF21, the LP diet-induced renal damage and inflammation were significantly worse, indicating a protective role of FGF21 in the kidney.

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Evaluation of the radio-protective role of PEG-Fe3O4 NPs on γ-irradiated male Wistar rats

Environmental Nanotechnology Monitoring & Management ◽

10.1016/j.enmm.2021.100620 ◽

2021 ◽

pp. 100620

Author(s):

Eman I. Sobeh ◽

Rehab Amin ◽

H.M. Saleh ◽

S.E. Ali ◽

Souad A. ElFeky

Keyword(s):

Wistar Rats ◽

Protective Role ◽

Fe3o4 Nps ◽

Male Wistar Rats

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