Bacterial respiratory tract inflammation in neonatal rat model is attenuated by benzofuran through inhibition of GATA3

Necrotizing enterocolitis (NEC) is the most common gastrointestinal disease of prematurely born infants. Maternal milk plays an important protective role against NEC development and is the major source of epidermal growth factor (EGF) for neonates. The aim of this study was to examine the effect of orally administered EGF on the incidence of NEC in a neonatal rat model. Newborn rats were artificially fed either with growth factor-free rat milk substitute (RMS) or RMS supplemented with 500 ng/ml of EGF (RMS+EGF). Experimental NEC was induced by exposure to asphyxia and cold stress. Development of NEC was evaluated by gross and histological scoring of damage in the ileum. Ileal EGF receptor (EGF-R), EGF, and transforming growth factor-α mRNA expression was assessed by RT competitive-PCR, and the EGF-R was localized by immunohistochemistry. EGF supplementation of formula reduced the incidence and severity of NEC in rats (13/16 RMS vs. 4/13 RMS+EGF). Ileal EGF-R mRNA expression was markedly increased in the RMS group compared with RMS+EGF. Enhanced EGF-R expression in the RMS group was localized predominantly in the epithelial cells of injured ileum. These data suggest a new potential therapeutic approach for the prevention and treatment of NEC.

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ISDN2014_0189: Sulforaphane is not additive in combination with hypothermia in a neonatal rat model of hypoxia–ischemia

International Journal of Developmental Neuroscience ◽

10.1016/j.ijdevneu.2015.04.155 ◽

2015 ◽

Vol 47 (Part_A) ◽

pp. 55-55 ◽

Cited By ~ 1

Author(s):

Ann‐Marie Przyslupski ◽

Edward Armstrong ◽

Keqin Shen ◽

Jerome Y. Yager

Keyword(s):

Rat Model ◽

Neonatal Rat ◽

Hypoxia Ischemia ◽

Neonatal Rat Model

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184 Intraperitoneal Cytidine 5'-Diphosphocholine (CDP-Choline) Administration Reduces the Severity of Intestinal Injury in a Neonatal Rat Model of Necrotizing Enterocolitis

Archives of Disease in Childhood ◽

10.1136/archdischild-2012-302724.0184 ◽

2012 ◽

Vol 97 (Suppl 2) ◽

pp. A53-A53

Author(s):

M. Cetinkaya ◽

M. Cansev ◽

F. Cekmez ◽

C. Tayman ◽

F. Canpolat ◽

...

Keyword(s):

Necrotizing Enterocolitis ◽

Rat Model ◽

Intestinal Injury ◽

Neonatal Rat ◽

Neonatal Rat Model

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Differential expressions of TGF-β1, HIF-1, VEGF, α-SMA and E-cadherin in renal tissues of a neonatal rat model of hydronephrosis

Tropical Journal of Pharmaceutical Research ◽

10.4314/tjpr.v20i9.11 ◽

2021 ◽

Vol 20 (9) ◽

pp. 1855-1860

Author(s):

Leibo Wang ◽

Jianhua He ◽

Ketao Xu ◽

Weichao Zhu ◽

Ketao Xu

Keyword(s):

Growth Factor ◽

Rat Model ◽

Muscle Protein ◽

Neonatal Rat ◽

Expression Level ◽

Neonatal Rats ◽

Model Group ◽

Tgf Β1 ◽

Neonatal Rat Model ◽

E Cadherin

Purpose: To investigate the differential expressions of transforming growth factor-β1 (TGF-β1), hypoxia inductive factor-1 (HIF-1), vascular endothelial growth factor (VEGF), α-smooth muscle protein (α-SMA) and E-cadherin in renal tissues of neonatal rat model of hydronephrosis.Methods: The neonatal rats (90) were randomly divided into sham group and model group. The rats in the model group were further divided into two subgroups: week 1 and week 12 after relief of obstruction, with 30 rats in each group. Six rats were taken from each group for the determination of renal histopathological changes. Levels of TGF-β1, HIF-1, VEGF, α-SMA and E-cadherin in renal tissues were compared for different pathological grades and at different time points of obstruction relief.Results: With increase in Elder grade, the concentrations of TGF-β1, HIF-1, VEGF and α-SMA in renal tissues of hydronephrosis neonatal rats were gradually increased, while the expression level of Ecadherin gradually decreased (p < 0.05). However, the concentrations of TGF-β1, HIF-1, VEGF and α-SMA in renal tissues were significantly reduced, while the expression level of E-adherin was upregulated with time after relief of obstruction (p < 0.05).Conclusion: These findings are of great significance in determining the degree of kidney injury and recovery, and for the development of drugs for the treatment of renal injury.

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Development and Optimization of a Neonatal Rat Model of Sepsis

Journal of Pediatrics & Neonatal Biology ◽

10.33140/jpnb.03.01.05 ◽

2018 ◽

Vol 3 (1) ◽

Keyword(s):

Blood Culture ◽

Rat Model ◽

Neonatal Sepsis ◽

Neonatal Rat ◽

Neonatal Rats ◽

Minimum Effective Dose ◽

Biochemical Tests ◽

E Coli ◽

Common Causes ◽

Neonatal Rat Model

Neonatal sepsis is the most common cause of mortality in newborns. Currently antibiotics and supportive care are the mainstay of treatment. Blood culture is considered as the gold standard for confirmation of diagnosis of neonatal sepsis. Here we have tried to develop a neonatal rat model of sepsis in order to better understand its progression. Lipopolysaccharide (LPS) is one of the common agents used to induce sepsis in rats. Here we found that LPS was ineffective in inducing sepsis in neonatal rats. We found that induction of live dose of Escherichia coli, one of the most common causes of neonatal sepsis was more effective than LPS injection. The rats were continuously monitored for the visual indications of sepsis development. Body weight, body temperature and the activity of rats were monitored continuously. Blood culture was done to check for the confirmation of diagnosis of sepsis. Further biochemical tests such as citrate, urease, indole and kliger-ion tests were done to confirm for E coli in the colonies of blood culture. The minimum effective dose of E coli needed to induce sepsis in neonatal rats was found to be 5*106 CFU of E coli.

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