Computer-assisted scanning electron microscopy of wood pulp fibres: Dimensions and spatial distributions in a polypropylene composite

Micron ◽  
2009 ◽  
Vol 40 (7) ◽  
pp. 761-768 ◽  
Author(s):  
G. Chinga-Carrasco ◽  
M. Lenes ◽  
P.O. Johnsen ◽  
E.-L. Hult
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Wood Pulp ◽  
Computer Assisted ◽  
Spatial Distributions ◽  
Polypropylene Composite ◽  
Pulp Fibres ◽  
Scanning Electron

Localization of Silver in the Spleen of Argyric Rats by Energy Dispersive X-Ray Analysis Coupled with Scanning and Transmission Electron Microscopy

1977 ◽  
Vol 35 ◽  
pp. 504-506
Author(s):  
G. Pereira
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Computer Assisted ◽  
White Pulp ◽  
Reticular Cell ◽  
X Ray ◽  
Transmission Electron ◽  
Freeze Dried ◽  
Scanning Electron

Previous electron microscopic observations of the spleen have revealed the white pulp to be completely separated from the extravasated blood in the surrounding marginal zone by a strategically-located, double layer of reticular cells ensheathing a coarse reticular fiber. Similarly, a single reticular cell layer has been observed to form a continuous investment for all white pulp capillaries. To test the significance of this apparent isolation of the splenic white pulp from the blood, the distribution and composition of silver deposits in the spleen of argyric rats were determined by transmission and scanning electron microscopy coupled with computer-assisted x-ray analysis.Young male albino rats were made argyric by supplying them for many months with drinking water to which 1.5gm per liter of silver nitrate had been added. Specimens from the spleens of control and argyric animals were prepared for conventional transmission electron microscopy by glutaraldehyde-osmium fixation. For scanning electron microscopy, other specimens were fixed in buffered glutaraldehyde, freeze-dried in vacuo, coated with a thin film of gold- palladium and examined in a Cambridge Stereoscan Mark II.

Morphometry characterisation of European eel spermatozoa with computer-assisted spermatozoa analysis and scanning electron microscopy

2006 ◽  
Vol 65 (7) ◽  
pp. 1302-1310 ◽  
Author(s):  
F. Marco-Jiménez ◽  
L. Pérez ◽  
M.P. Viudes de Castro ◽  
D.L. Garzón ◽  
D.S. Peñaranda ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Computer Assisted ◽  
European Eel ◽  
Scanning Electron

Chitosan-Coated Fibers for Use in Papermaking

1990 ◽  
Vol 197 ◽  
Author(s):  
G. G. Allan ◽  
J. P. Carroll ◽  
Y. Hirabayashi ◽  
M. Muvundamina ◽  
J. G. Winterowd
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Glass Fibers ◽  
Wood Pulp ◽  
Polypropylene Fibers ◽  
Pulp Fibers ◽  
Scanning Electron

ABSTRACTChitosan was precipitated onto wood pulp, glass and polypropylene fibers suitable for papermaking. The coating which formed on the fibers was characterized by photomicrography after the formation of colored derivatives, and by scanning electron microscopy. The most uniform and adherent coatings were formed on the wood pulp fibers and the least were formed on the polypropylene fibers. Paper handsheets were readily formed from the chitosan-coated wood pulp and glass fibers, but not from the chitosan-coated polypropylene fibers.

Investigation of the tridimensional ultrastructure of rat glomerular capillary endothelium by high-resolution scanning electron microscopy

1990 ◽  
Vol 48 (3) ◽  
pp. 30-31
Author(s):  
P.J. Lea ◽  
R.J. Temkin ◽  
T. Banoub ◽  
M. Silverman ◽  
M.J. Hollenberg
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
High Resolution ◽  
Three Dimensions ◽  
Capillary Endothelium ◽  
Computer Assisted ◽  
Thin Sections ◽  
Glomerular Capillary ◽  
Kidney Glomerulus ◽  
Scanning Electron

The principal applications of scanning electron microscopy (SEM) to renal ultrastructure have been in the study of the surface topography of various kidney cell types and their orientation and distribution in both health and disease. SEM study, however, has been limited in a major way by a lack of resolution sufficient to readily examine in detail and in three dimensions such features as glomerular basement membrane substructure and the structural organization at high magnification of the glomerular, capillary endothelium. Consequently, most of our current information about the 3D ultrastructure of rat kidney glomerulus has been obtained from transmission electron (TEM) micrographs obtained from thin sections cut at various planes followed by computer assisted, serial reconstructions. Recent advances in specimen preparation techniques and scanning electron microscope design have permitted ultrastructural examination of the glomerular capillary wall in three dimensions using high resolution scanning electron microscopy (HRSEM). Specimens in which the cytosol and cytoskeleton have been extracted, but cell membranes nuclear structures and organelles left in place, were studied using a Hitachi SEM with a resolution of approximately 3 nm.

Spatial and temporal thyrocyte response to TSH: a computer-assisted image analysis

1992 ◽  
Vol 263 (2) ◽  
pp. E231-E238
Author(s):  
C. Penel ◽  
J. Mauchamp
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Image Analysis ◽  
Computer Assisted ◽  
Two Dimensional ◽  
Experimental Distribution ◽  
Computer Assisted Image ◽  
Two Populations ◽  
Tsh Stimulation ◽  
Scanning Electron

Pseudopods at the apical pole of porcine thyroid monolayers in culture were considered as reflecting individual thyrocyte responses to thyrotropin (TSH) stimulation. Scanning electron microscopy and computer-assisted image analysis showed that whatever TSH stimulation was used, the pseudopods were characterized by two populations: P1 with small diameters (2 microns) and P2 with greater diameters (5 microns). The density of P1 rapidly increased to reach a plateau, while P2 continuously increased during stimulation. Two-dimensional pseudopod patterns were compared with random point distributions by means of two topographical parameters: the interpseudopod distances and angles. A factorial analysis of experimental distribution of pseudopods obtained after increasing stimulation times displayed a shift from a nonrandom (10-20 min) to a random (60-90 min) distribution. Clusters of three pseudopods characterized by short distances (6-9 microns) and equilateral organization (angles 40-60 degrees) were observed after a 10-min stimulation. These results suggested that early thyrocyte response to TSH stimulation is characterized by interrelations between three adjoining cells, with the thyrocyte response later appearing as random.

Pathogenesis of Human Hair Defects

1974 ◽  
Vol 32 ◽  
pp. 12-13
Author(s):  
P.S. Porter ◽  
T. Aoyagi ◽  
R. Matta
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Human Hair ◽  
Standard Techniques ◽  
Scanning Electron

Using standard techniques of scanning electron microscopy (SEM), over 1000 human hair defects have been studied. In several of the defects, the pathogenesis of the abnormality has been clarified using these techniques. It is the purpose of this paper to present several distinct morphologic abnormalities of hair and to discuss their pathogenesis as elucidated through techniques of scanning electron microscopy.

Ultrastructural Analysis of the Salivary Glands of Gromphadorhina Portentosa (Schaum)

1977 ◽  
Vol 35 ◽  
pp. 638-639
Author(s):  
P.J. Dailey
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Salivary Glands ◽  
Secretory Vesicles ◽  
The Past ◽  
Transmission Electron ◽  
Means Of Transmission ◽  
Gromphadorhina Portentosa ◽  
Scanning Electron ◽  
Topographical Relief

The structure of insect salivary glands has been extensively investigated during the past decade; however, none have attempted scanning electron microscopy (SEM) in ultrastructural examinations of these secretory organs. This study correlates fine structure by means of SEM cryofractography with that of thin-sectioned epoxy embedded material observed by means of transmission electron microscopy (TEM).Salivary glands of Gromphadorhina portentosa were excised and immediately submerged in cold (4°C) paraformaldehyde-glutaraldehyde fixative1 for 2 hr, washed and post-fixed in 1 per cent 0s04 in phosphosphate buffer (4°C for 2 hr). After ethanolic dehydration half of the samples were embedded in Epon 812 for TEM and half cryofractured and subsequently critical point dried for SEM. Dried specimens were mounted on aluminum stubs and coated with approximately 150 Å of gold in a cold sputtering apparatus.Figure 1 shows a cryofractured plane through a salivary acinus revealing topographical relief of secretory vesicles.

Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

1978 ◽  
Vol 36 (2) ◽  
pp. 82-83 ◽  
Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Thin Sections ◽  
Transmission Electron ◽  
Identical Cell ◽  
Electron Microscopes ◽  
Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

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