Sterol profile of Neobenedenia melleni, a marine ectoparasite fish

Analysis of plant sterols in pulp mill effluents is complex and several investigations have combined wood extractives with sterols or have reported total sterols collectively. The present study exclusively focuses on the fate of individual sterols generated in pulp and paper manufacturing as they pass through activated sludge biotreatment system. Before secondary treatment the pulp mill effluents contained 33% ß-sitosterol, 26% campesterol, 22% ß-sitostanol, 17% stigmasterol and 2% ergosterol (1,000–1,800 μg/L mean total sterols). After treatment, the effluents contained 44% ß-sitosterol, 22% campesterol, 18% ß-sitostanol, 14% stigmasterol and 2% ergosterol (176–428 μg/L mean total sterols). Each sterol fraction showed different removal efficiency. ß-Sitosterol, the major fraction, was removed relatively poorly (65%) while campesterol was removed most efficiently (81%) compared with the removal of other fractions (ß-sitostanol 74% and stigmasterol 64%). The differential removal of sterol fractions altered the sterol profile during different stages of the secondary treatment. Owing to its poor removal, ß-sitosterol is the most persistent fraction in treated/untreated pulp mill effluents. Typically, 21% of the incoming sterols were contained in secondary effluents and 23% in waste sludge without biodegradation. Optimizing the design/operation of treatment systems for removal of ß-sitosterol and stigmasterol would improve the performance of effluent treatment facilities.

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Altered sterol profile induced in Leishmania amazonensis by a natural dihydroxymethoxylated chalcone

Journal of Antimicrobial Chemotherapy ◽

10.1093/jac/dkn546 ◽

2009 ◽

Vol 63 (3) ◽

pp. 469-472 ◽

Cited By ~ 25

Author(s):

E. C. Torres-Santos ◽

M. I. Sampaio-Santos ◽

F. S. Buckner ◽

K. Yokoyama ◽

M. Gelb ◽

...

Keyword(s):

Leishmania Amazonensis ◽

Sterol Profile

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Pyridines and Pyrimidines Mediating Activity against an Efflux-Negative Strain of Candida albicans through Putative Inhibition of Lanosterol Demethylase

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.48.1.313-318.2004 ◽

2004 ◽

Vol 48 (1) ◽

pp. 313-318 ◽

Cited By ~ 13

Author(s):

Ed T. Buurman ◽

April E. Blodgett ◽

Kenneth G. Hull ◽

Daniel Carcanague

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Ergosterol Biosynthesis ◽

Clear Correlation ◽

Wild Type ◽

Inhibition Of Growth ◽

Ergosterol Synthesis ◽

A Cell ◽

Sterol Profile ◽

Lanosterol Demethylase

ABSTRACT The first step in ergosterol biosynthesis in Saccharomyces cerevisiae consists of the condensation of two acetyl coenzyme A (acetyl-CoA) moieties by acetoacetyl-CoA thiolase, encoded by ERG10. The inhibition of the sterol pathway results in feedback activation of ERG10 transcription. A cell-based reporter assay, in which increased ERG10 transcription results in elevated specific β-galactosidase activity, was used to find novel inhibitors of ergosterol biosynthesis that could serve as chemical starting points for the development of novel antifungal agents. A class of pyridines and pyrimidines identified in this way had no detectable activity against the major fungal pathogen Candida albicans (MICs > 64 μg · ml−1). However, a strain of C. albicans lacking the Cdr1p and Cdr2p efflux pumps was sensitive to the compounds (with MICs ranging from 2 to 64 μg · ml−1), suggesting that they are efficiently removed from wild-type cells. Quantitative analysis of sterol intermediates that accumulated during growth inhibition revealed the accumulation of lanosterol at the expense of ergosterol. Furthermore, a clear correlation was found between the 50% inhibitory concentration at which the sterol profile was altered and the antifungal activity, measured as the MIC. This finding strongly suggests that the inhibition of growth was caused by a reduction in ergosterol synthesis. The compounds described here are a novel class of antifungal pyridines and pyrimidines and the first pyri(mi)dines to be shown to putatively mediate their antifungal activity against C. albicans via lanosterol demethylase.

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Sterol profile of some medicinal and aromatic plants: Effect of silyl derivatization step

European Journal of Science and Technology ◽

10.31590/ejosat.597071 ◽

2019 ◽

pp. 360-365

Author(s):

Kevser Karaman ◽

Erman Beyzi ◽

Selma Büyükkılıç Beyzi

Keyword(s):

Aromatic Plants ◽

Medicinal And Aromatic Plants ◽

Sterol Profile ◽

Silyl Derivatization

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The role of sterols in plant response to abiotic stress

Phytochemistry Reviews ◽

10.1007/s11101-020-09708-2 ◽

2020 ◽

Vol 19 (6) ◽

pp. 1525-1538 ◽

Cited By ~ 3

Author(s):

Agata Rogowska ◽

Anna Szakiel

Keyword(s):

Stress Response ◽

Abiotic Factors ◽

Plant Stress ◽

Membrane Lipid ◽

Response Diversity ◽

Membrane Lipid Bilayer ◽

Sterol Profile ◽

Plant Cell Membranes ◽

Regulation Of Growth

AbstractSterols are integral components of the membrane lipid bilayer and they are involved in many processes occurring in plants, ranging from regulation of growth and development to stress resistance. Maintenance of membrane homeostasis represents one of the principal functions of sterols in plant cells. Plant cell membranes are important sites of perception of environmental abiotic factors, therefore, it can be surmised that sterols may play an important role in the plant stress response. The aim of this review was to discuss the most representative trends in recent studies regarding the role of sterols in plant defense reactions to environmental factors, such as UV radiation, cold and drought stress. Some correlations were observed between changes in the sterol profile, referring to the ratios of individual compounds (including 24-methyl/ethyl sterols and sitosterol/stigmasterol) as well as the relative proportions of conjugated sterols (ASGs, SGs and SEs) and the nature of the stress response. Diversity of sterols and their conjugated forms may allow sessile plants to adapt to environmental stress conditions.

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Imipramine alters the sterol profile in Leishmania amazonensis and increases its sensitivity to miconazole

Parasites & Vectors ◽

10.1186/s13071-016-1467-8 ◽

2016 ◽

Vol 9 (1) ◽

Cited By ~ 10

Author(s):

Valter Viana Andrade-Neto ◽

Thaís Martins Pereira ◽

Marilene do Canto-Cavalheiro ◽

Eduardo Caio Torres-Santos

Keyword(s):

Leishmania Amazonensis ◽

Sterol Profile

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Microbial Symbionts Shape the Sterol Profile of the Xylem-Feeding Woodwasp, Sirex noctilio

Journal of Chemical Ecology ◽

10.1007/s10886-012-0222-7 ◽

2012 ◽

Vol 39 (1) ◽

pp. 129-139 ◽

Cited By ~ 33

Author(s):

Brian M. Thompson ◽

Robert J. Grebenok ◽

Spencer T. Behmer ◽

Daniel S. Gruner

Keyword(s):

Sirex Noctilio ◽

Sterol Profile ◽

Microbial Symbionts

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The Distribution of Sterols and Stanols in the Tunicate Ciona Intestinalis

Journal of the Marine Biological Association of the United Kingdom ◽

10.1017/s0025315400020142 ◽

1982 ◽

Vol 62 (1) ◽

pp. 117-123 ◽

Cited By ~ 10

Author(s):

R. J. Morris ◽

M. J. Mccartney ◽

Q. Bone

Keyword(s):

Natural Product ◽

Marine Invertebrates ◽

Wide Spectrum ◽

Packed Column ◽

Physiological Role ◽

Mass Spectral ◽

The Common ◽

Sterol Profile ◽

Liquid Chromatographic ◽

Microbiological Reduction

INTRODUCTIONUntil recently saturated sterols – stands – were not thought to play an active biochemical or physiological role in organisms. They were generally considered to be diagenetic products of the microbiological reduction of the common natural product Δ5sterols and as such have been reported in sediments (e.g. Gaskell & Eglinton, 1975).Prior to the regular use of capillary gas liquid chromatographic (GLC) and gas chromatographic-mass spectrometric (GC-MS) techniques the majority of sterol analyses had been performed on packed column GLC. It is now well known that in the analysis of some of the complex mixtures of sterols which commonly occur in the marine environment, the use of packed column GLC techniques alone is an inadequate procedure (e.g. Ballantine; Roberts & Morris, 1976; Ballantine et al. 1977). Certainly complete resolution of the sterol-stanol pairs cannot normally be achieved on packed columns and identification of the components within each peak has to be made by multiple mass spectral scans across each peak (Ballantine et al. 1976). With the advent of techniques with better resolving powers, significant amounts of natural product stands have been found in a wide spectrum of phyla including molluscs, sponges coelenterates, echinoderms, annelids and tunicates (Morris & Culkin, 1977 and references therein; Ballantine et al. 1976; Ballantine, Lavis & Morris, 1979, 1981; Voogt, 1976; Ballantine et al. 1977; Ballantine et al. 1978; Gupta et al. 1979). In some instances the stanols have been found to account for over 50% of the animals component sterols.As the sterol profile of marine invertebrates becomes better understood it is clear that stanols are important biochemical constituents of many of these organisms; but little is known of their significance in any biochemical or physiological role.

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