Immunological characterization of rLdTCP1γ for its prophylactic potential against visceral leishmaniasis in hamster model

SummaryNine human antibodies to factor VIII were isolated from haemophilic plasmas by affinity chromatography and gel filtration and six were subsequently subjected to immunological characterization. Three partially purified preparations were similarly characterized. Eight of the antibodies were characterized as being exclusively IgG and one preparation was found to contain IgM. Seven of the antibodies contained only a single light chain type, four being of type lambda and three of type kappa. Two antibody preparations contained both kappa and lambda light chains. In four of the preparations, only a single heavy chain sub-class could be demonstrated, three of IgG3 and one of IgG4. Of the remainder, three were a mixture of IgG3 and IgG4 sub-classes and one contained both IgG2 and IgG4. IgG sub-classification could not be achieved with the IgM-containing preparation. These results demonstrate a restricted heterogeneity of light and heavy chains in human antibodies to factor VIII.

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Immunological characterization of immunoglobulin positive cells - Study in secondary syphilis and chronic inflammatory disorders.

Nishi Nihon Hifuka ◽

10.2336/nishinihonhifu.49.285 ◽

1987 ◽

Vol 49 (2) ◽

pp. 285-289

Author(s):

Yoko NAGATA

Keyword(s):

Secondary Syphilis ◽

Immunological Characterization ◽

Inflammatory Disorders

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Conventional Versus Natural Alternative Treatments for Leishmaniasis: A Review

Current Topics in Medicinal Chemistry ◽

10.2174/1568026618666181002114448 ◽

2018 ◽

Vol 18 (15) ◽

pp. 1275-1286 ◽

Cited By ~ 12

Author(s):

Luiz Felipe Domingues Passero ◽

Lucas Antal Cruz ◽

Gabriela Santos-Gomes ◽

Eliana Rodrigues ◽

Márcia Dalastra Laurenti ◽

...

Keyword(s):

Side Effects ◽

Visceral Leishmaniasis ◽

Traditional Knowledge ◽

Conventional Therapy ◽

Pathogenic Species ◽

Action Mechanism ◽

In Vivo Assays ◽

Clinical Forms

Leishmaniasis is a neglected disease caused by protozoan belonging to the Leishmania genus. There are at least 16 pathogenic species for humans that are able to cause different clinical forms, such as cutaneous or visceral leishmaniasis. In spite of the different species and clinical forms, the treatment is performed with few drug options that, in most cases, are considered outdated. In addition, patients under classical treatment show serious side effects during drug administration, moreover parasites are able to become resistant to medicines. Thus, it is believed and well accepted that is urgent and necessary to develop new therapeutic options to overpass these concerns about conventional therapy of leishmaniasis. The present review will focus on the efficacy, side effects and action mechanism of classic drugs used in the treatment of leishmaniasis, as well as the importance of traditional knowledge for directing a rational search toward the discovery and characterization of new and effective molecules (in vivo assays) from plants to be used against leishmaniasis.

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