Imbalance in pro-inflammatory and anti-inflammatory cytokines milieu in B cells of children with autism

2022 ◽  
Vol 141 ◽  
pp. 297-304
Author(s):  
Ahmed Nadeem ◽  
Sheikh F. Ahmad ◽  
Naif O. Al-Harbi ◽  
Laila Y. AL-Ayadhi ◽  
Wedad Sarawi ◽  
...  
Keyword(s):  
B Cells ◽  
Inflammatory Cytokines ◽  
Children With Autism ◽  
Anti Inflammatory

scholarly journals CD19+ CD24hi CD38hi Regulatory B Cells and Memory B Cells in Periodontitis: Association with Pro-Inflammatory and Anti-Inflammatory Cytokines

Vaccines ◽  
2020 ◽  
Vol 8 (2) ◽  
pp. 340
Author(s):  
Helal F. Hetta ◽  
Ibrahim M. Mwafey ◽  
Gaber El-Saber Batiha ◽  
Suliman Y. Alomar ◽  
Nahed A. Mohamed ◽  
...  
Keyword(s):  
B Cells ◽  
Inflammatory Cytokines ◽  
Periodontal Diseases ◽  
Serum Levels ◽  
Peripheral Tolerance ◽  
Regulatory B Cells ◽  
Tnf Α ◽  
Cytokine Levels ◽  
Anti Inflammatory ◽  
Pro Inflammatory Cytokines

Regulatory B cells (Bregs) are unique subpopulations of B cells with immune-regulating or immune-suppressing properties and play a role in peripheral tolerance. Due to the current limitations of human Breg studies among periodontal diseases, in the present study, we tried to analyze the change in circulating Bregs, pro-inflammatory, and anti-inflammatory cytokines in patients with periodontitis. Peripheral blood from 55 patients with stage 2 periodontitis and 20 healthy controls was analyzed using flow cytometry to evaluate the frequency of CD19+CD24+CD38+ Breg cells. ELISA was used to assess the serum levels of the pro-inflammatory cytokines, including interleukins (IL)-1β, IL-6, TNF-α, and anti-inflammatory cytokines including IL-10, IL-35, and TGF-β. Increased proportions of Breg cells were observed in patients with stage 2 periodontitis compared to controls. Serum levels of cytokines were significantly higher in patients with periodontitis compared to controls. A significant positive correlation was observed between the frequencies of Breg cells and IL35 levels, IL10 levels, and TGF-β. In conclusion, our results suggest that the increase in peripheral Breg cells and serum cytokine levels among periodontitis patients seems to be closely associated with disease progression, a possible link between periodontitis, and systemic inflammatory process.

Increased viability of cardiomyocytes after exposure of anti-inflammatory cytokines

2008 ◽  
Vol 56 (S 1) ◽  
Author(s):  
W Rees ◽  
T Kubin ◽  
J Pöling ◽  
S Hein ◽  
H Warnecke ◽  
...  
Keyword(s):  
Inflammatory Cytokines ◽  
Anti Inflammatory

AAnti-Inflammatory Effects of Plasma Circulating Exosomes Obtained from Normal-Weight and Obese Subjects on Hepatocytes

2020 ◽  
Vol 20 ◽  
Author(s):  
Reza Afrisham ◽  
Sahar Sadegh-Nejadi ◽  
Reza Meshkani ◽  
Solaleh Emamgholipour ◽  
Molood Bagherieh ◽  
...  
Keyword(s):  
Inflammatory Cytokines ◽  
Hepg2 Cells ◽  
Human Liver ◽  
Liver Cells ◽  
Normal Weight ◽  
Protein Levels ◽  
Human Liver Cells ◽  
Tnf Α ◽  
Anti Inflammatory

Introduction: Obesity is a disorder with low-grade chronic inflammation that plays a key role in the hepatic inflammation and steatosis. Moreover, there are studies to support the role of exosomes in the cellular communications, the regulation of metabolic homeostasis and immunomodulatory activity. Accordingly, we aimed to evaluate the influence of plasma circulating exosomes derived from females with normal-weight and obesity on the secretion of inflammatory cytokines in human liver cells. Methods: Plasma circulating exosomes were isolated from four normal (N-Exo) and four obese (O-Exo) women. The exosomes were characterized and approved for CD63 expression (common exosomal protein marker) and morphology/size using the western blot and TEM methods, respectively. The exosomes were used for stimulation of HepG2 cells in vitro. After 24 h incubation, the protein levels of TNF-α,IL-6, and IL-1β were measured in the culture supernatant of HepG2 cells using the ELISA kit. Results: The protein levels of IL-6 and TNF-α in the cells treated with O-Exo and N-Exo reduced significantly in comparison with control group (P=0.039 and P<0.001 respectively), while significance differences were not found between normal and obese groups (P=0.808, and P=0.978 respectively). However, no significant differences were found between three groups in term of IL-1β levels (P=0.069). Based on the correlation analysis, the protein levels of IL-6 were positively correlated with TNF-α (r 0.978, P<0.001). Conclusion: These findings suggest that plasma circulating exosomes have probably anti-inflammatory properties independently from body mass index and may decrease the secretion of inflammatory cytokines in liver. However, further investigations in vitro and in vivo are needed to address the anti-inflammatory function of N-Exo and O-Exo in human liver cells and/or other cells.

Predictive value of pro- and anti-inflammatory cytokines in assessing severity and outcome of acute pancreatitis

2005 ◽  
Vol 24 (4) ◽  
pp. 253-257 ◽  
Author(s):  
Darko Mirkovic ◽  
Maja Surbatovic ◽  
Nikola Filipovic ◽  
Sonja Radakovic
Keyword(s):  
Acute Pancreatitis ◽  
Inflammatory Cytokines ◽  
Predictive Value ◽  
Anti Inflammatory

Induction of inflammatory and anti-inflammatory cytokines in microglia

2011 ◽  
Vol 71 ◽  
pp. e327
Author(s):  
Tomohiro Saito ◽  
Kenji Sudo ◽  
Hiroaki Miyamori ◽  
Kazuyuki Nakajima
Keyword(s):  
Inflammatory Cytokines ◽  
Anti Inflammatory
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