scholarly journals ZmMs30 Encoding a Novel GDSL Lipase Is Essential for Male Fertility and Valuable for Hybrid Breeding in Maize

2019 ◽  
Vol 12 (3) ◽  
pp. 343-359 ◽  
Author(s):  
Xueli An ◽  
Zhenying Dong ◽  
Youhui Tian ◽  
Ke Xie ◽  
Suowei Wu ◽  
...  
Keyword(s):  
Male Fertility ◽  
Hybrid Breeding ◽  
Gdsl Lipase

scholarly journals TaEXPB5 is responsible for male fertility in thermo-sensitive male-sterility wheat with Aegilops kotschyi cytoplasm

2021 ◽  
Author(s):  
Xingxia Geng ◽  
Xiaoxia Wang ◽  
Jingchen Wang ◽  
Xuetong Yang ◽  
lingli zhang ◽  
...  
Keyword(s):  
Male Sterility ◽  
Pollen Development ◽  
Male Fertility ◽  
Crop Production ◽  
Hybrid Vigor ◽  
Abnormal Development ◽  
Hybrid Breeding ◽  
Pollen Abortion ◽  
Gene Encoding ◽  
Early Degradation

Thermo-sensitive male sterility is of vital importance to heterosis, or hybrid vigor in crop production and hybrid breeding. Therefore, it is meaningful to study the function of the genes related to pollen development and male sterility, which is still not fully understand currently. Here, we conducted comparative analyses to screen fertility related genes using RNA-seq, iTRAQ, and PRM-based assay. A gene encoding expansin protein in wheat, TaEXPB5, was isolated in KTM3315A, which was in the cell wall and preferentially upregulated expression in the fertility anthers. The silencing of TaEXPB5 displayed pollen abortion, the declination or sterility of fertility. Further, cytological investigation indicated that the silencing of TaEXPB5 induced the early degradation of tapetum and abnormal development of pollen wall. These results revealed that the silencing of TaEXPB5 could eliminate the effects of temperature on male fertility, and resulting in functional loss of fertility conversion, which implied that TaEXPB5 may be essential for anther or pollen development and male fertility of KTM3315A. These findings provide a novel insight into molecular mechanism of fertility conversion for thermo-sensitive cytoplasmic male-sterility wheat, and contribute to the molecular breeding of hybrid wheat in the future.

scholarly journals RMS2 Encoding a GDSL Lipase Mediates Lipid Homeostasis in Anthers to Determine Rice Male Fertility

2020 ◽  
Vol 182 (4) ◽  
pp. 2047-2064 ◽  
Author(s):  
Juan Zhao ◽  
Tuan Long ◽  
Yifeng Wang ◽  
Xiaohong Tong ◽  
Jie Tang ◽  
...  
Keyword(s):  
Male Fertility ◽  
Lipid Homeostasis ◽  
Gdsl Lipase

scholarly journals Genomic Scan of Male Fertility Restoration Genes in a ‘Gülzow’ Type Hybrid Breeding System of Rye (Secale cereale L.)

2021 ◽  
Vol 22 (17) ◽  
pp. 9277
Author(s):  
Nikolaj Meisner Vendelbo ◽  
Khalid Mahmood ◽  
Pernille Sarup ◽  
Peter Skov Kristensen ◽  
Jihad Orabi ◽  
...  
Keyword(s):  
Breeding System ◽  
Secale Cereale ◽  
Male Fertility ◽  
Association Studies ◽  
Type Systems ◽  
Hybrid Breeding ◽  
Genome Wide Association Studies ◽  
Hybrid Seed Production ◽  
Rf Gene ◽  
Secale Cereale L

Efficient and stable restoration of male fertility (Rf) is a prerequisite for large-scale hybrid seed production but remains an inherent issue in the predominant fertility control system of rye (Secale cereale L.). The ‘Gülzow’ (G)-type cytoplasmic male sterility (CMS) system in hybrid rye breeding exhibits a superior Rf. While having received little scientific attention, one major G-type Rf gene has been identified on 4RL (Rfg1) and two minor genes on 3R (Rfg2) and 6R (Rfg3) chromosomes. Here, we report a comprehensive investigation of the genetics underlying restoration of male fertility in a large G-type CMS breeding system using recent advents in rye genomic resources. This includes: (I) genome-wide association studies (GWAS) on G-type germplasm; (II) GWAS on a biparental mapping population; and (III) an RNA sequence study to investigate the expression of genes residing in Rf-associated regions in G-type rye hybrids. Our findings provide compelling evidence of a novel major G-type non-PPR Rf gene on the 3RL chromosome belonging to the mitochondrial transcription termination factor gene family. We provisionally denote the identified novel Rf gene on 3RL RfNOS1. The discovery made in this study is distinct from known P- and C-type systems in rye as well as recognized CMS systems in barley (Hordeum vulgare L.) and wheat (Triticum aestivum L.). We believe this study constitutes a stepping stone towards understanding the restoration of male fertility in the G-type CMS system and potential resources for addressing the inherent issues of the P-type system.

scholarly journals Map-Based Cloning, Phylogenetic, and Microsynteny Analyses of ZmMs20 Gene Regulating Male Fertility in Maize

2019 ◽  
Vol 20 (6) ◽  
pp. 1411 ◽  
Author(s):  
Yanbo Wang ◽  
Dongcheng Liu ◽  
Youhui Tian ◽  
Suowei Wu ◽  
Xueli An ◽  
...  
Keyword(s):  
Male Sterility ◽  
Pollen Development ◽  
Male Fertility ◽  
Molecular Mechanisms ◽  
Expression Patterns ◽  
Hybrid Breeding ◽  
Functional Markers ◽  
Hybrid Seed Production ◽  
Functional Relationships ◽  
Zea May

Genic male sterility (GMS) mutant is a useful germplasm resource for both theory research and production practice. The identification and characterization of GMS genes, and assessment of male-sterility stability of GMS mutant under different genetic backgrounds in Zea may (maize) have (1) deepened our understanding of the molecular mechanisms controlling anther and pollen development, and (2) enabled the development and efficient use of many biotechnology-based male-sterility (BMS) systems for hybrid breeding. Here, we reported a complete GMS mutant (ms20), which displays abnormal anther cuticle and pollen development. Its fertility restorer gene ZmMs20 was found to be a new allele of IPE1 encoding a glucose methanol choline (GMC) oxidoreductase involved in lipid metabolism in anther. Phylogenetic and microsynteny analyses showed that ZmMs20 was conserved among gramineous species, which provide clues for creating GMS materials in other crops. Additionally, among the 17 maize cloned GMS genes, ZmMs20 was found to be similar to the expression patterns of Ms7, Ms26, Ms6021, APV1, and IG1 genes, which will give some clues for deciphering their functional relationships in regulating male fertility. Finally, two functional markers of ZmMs20/ms20 were developed and tested for creating maize ms20 male-sterility lines in 353 genetic backgrounds, and then an artificial maintainer line of ms20 GMS mutation was created by using ZmMs20 gene, ms20 mutant, and BMS system. This work will promote our understanding of functional mechanisms of male fertility and facilitate molecular breeding of ms20 male-sterility lines for hybrid seed production in maize.

scholarly journals Molecular identification of the wheat male fertility gene Ms1 and its prospects for hybrid breeding

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Elise J. Tucker ◽  
Ute Baumann ◽  
Allan Kouidri ◽  
Radoslaw Suchecki ◽  
Mathieu Baes ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Male Fertility ◽  
Hybrid Breeding ◽  
Fertility Gene

scholarly journals Fine Mapping and Candidate Gene Analysis For A Novel Male-Sterile Mutant Ms40 in Maize

2021 ◽  
Author(s):  
Liu Xiaowei ◽  
Yue Yujing ◽  
Gu Zicheng ◽  
Huang Qing ◽  
Pan Zijin ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Male Fertility ◽  
Marker Assisted Selection ◽  
Nuclear Gene ◽  
Hybrid Breeding ◽  
Bhlh Transcription Factor ◽  
Chromosome 4 ◽  
Male Sterile ◽  
Sterile Mutant ◽  
Selection Of

Abstract Maize male sterile mutant 40 (ms40) was obtained from the progeny of ethyl methanesulfonate (EMS) treated inbred line RP125. Genetic analysis showed that it was controlled by a single recessive nuclear gene. Cytological observation of anthers revealed that abnormal cuticles and disappearing of Ubisch bodies presented in ms40. Moreover, its tapetum exhibited delayed degradation and blocked the formation of abnormal microspore. Using map-based cloning, ms40 locus was located in a 282-kb interval on chromosome 4, five annotated genes were predicted within this region. PCR-based sequencing detected a single nonsynonymous SNP (G>A) which changed glycine (G) to arginine (A) in the seventh exon of Zm00001d053895, while no difference was found for the other four genes between ms40 and RP125. Zm00001d053895 encodes the bHLH transcription factor bHLH51 which protein was located at nuclear. Phylogenetic analysis presented that bHLH51 had the highest homology with Sb04g001650, a tapetum degeneration retardation (TDR) bHLH transcription factor in Sorghum bicolor. Co-expression analysis exposed a total of 1192 genes coexpressed with Zm00001d053895 in maize, 647 out of 1192 were anther-specific genes. In summary, these findings are conducive to the marker-assisted selection of ms40 in hybrid breeding and laid a foundation for further studies on the mechanisms of male fertility.

scholarly journals Identification of fertility-related genes for maize CMS-S via Bulked Segregant RNA-Seq

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e10015
Author(s):  
Xiner Qin ◽  
Wenliang Zhang ◽  
Xue Dong ◽  
Shike Tian ◽  
Panpan Zhang ◽  
...  
Keyword(s):  
Male Fertility ◽  
Function Analysis ◽  
Fertility Restoration ◽  
Signal Pathway ◽  
Hybrid Breeding ◽  
Rna Seq ◽  
Male Sterile ◽  
Maize Hybrid ◽  
Hybrid Production ◽  
Two Populations

Cytoplasmic male sterility (CMS) is extensively used in maize hybrid production, and identification of genes related to fertility restoration for CMS is important for hybrid breeding. The fertility restoration of S type CMS is governed by several loci with major and minor effects, while the mechanism of fertility restoration for CMS-S is still unknown. In this study, BSR-Seq was conducted with two backcrossing populations with the fertility restoration genes, Rf3 and Rf10, respectively. Genetic mapping via BSR-Seq verified the positions of the two loci. A total of 353 and 176 differentially expressed genes (DEGs) between the male fertility and male sterile pools were identified in the populations with Rf3 and Rf10, respectively. In total, 265 DEGs were co-expressed in the two populations, which were up-regulated in the fertile plants, and they might be related to male fertility involving in anther or pollen development. Moreover, 35 and seven DEGs were specifically up-regulated in the fertile plants of the population with Rf3 and Rf10, respectively. Function analysis of these DEGs revealed that jasmonic acid (JA) signal pathway might be involved in the Rf3 mediated fertility restoration for CMS-S, while the small ubiquitin-related modifier system could play a role in the fertility restoration of Rf10.

scholarly journals IRREGULAR POLLENEXINE2 Encodes a GDSL Lipase Essential for Male Fertility in Maize

2020 ◽  
Vol 184 (3) ◽  
pp. 1438-1454
Author(s):  
Yanqing Huo ◽  
Yuanrong Pei ◽  
Youhui Tian ◽  
Zhaogui Zhang ◽  
Kai Li ◽  
...  
Keyword(s):  
Male Fertility ◽  
Gdsl Lipase

scholarly journals Genetic architecture of male fertility restoration in a hybrid breeding system of rye (Secale cereale L.)

2021 ◽  
Author(s):  
Nikolaj Vendelbo ◽  
Khalid Mahmood ◽  
Pernille Sarup ◽  
Peter Kristensen ◽  
Jihad Orabi ◽  
...  
Keyword(s):  
Breeding System ◽  
Secale Cereale ◽  
In Silico ◽  
Male Fertility ◽  
Fertility Restoration ◽  
Association Studies ◽  
Type Systems ◽  
Hybrid Breeding ◽  
Genome Wide Association Studies ◽  
Male Fertility Restoration

Abstract The ‘Gülzow’ (G) type cytoplasmic male sterility (CMS) system in hybrid rye (Secale cereale L.) breeding exhibits a strong and environmentally stable restoration of male fertility (Rf). While having received little scientific attention, three G-type Rf genes had been identified on 4RL (Rfg1) and two minor genes on 3R (Rfg2) and 6R (Rfg3) chromosome. Here, we report a comprehensive investigation of the genetics underlying restoration of male fertility in a large G-type CMS breeding system using a palette of complementing forward and reverse genetic analysis. This includes (i) genome wide association studies (GWAS) on a G-type germplasm, (ii) GWAS on a biparental mapping population, (iii) in silico identification of Rf-like pentatricopeptide repeat (RFL-PPR) genes and their expressed in G-type rye hybrids, and (iv) mining patterns in linkage disequilibrium. Our findings provide compelling evidence of a novel major G-type non-PPR Rf gene on the 3RL chromosome. In the in silico analysis, we identified 22 RFL-PPR of which 15 were expressed in the transcriptome of G-type hybrids. Our findings provides a novel insight into the underlying genetics of male fertility restoration in a G-type CMS system in rye. The discovery made in this study is distinct to known P- and C-type systems in rye in addition to known CMS systems in barley and wheat. This study constitutes a steppingstone towards understanding the restoration of male fertility in G-type CMS system and a potential resources for addressing the inherent issues of the P-type system.

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